Imaging α4β2 Nicotinic Acetylcholine Receptors (nAChRs) in Baboons with [18F]XTRA, a Radioligand with Improved Specific Binding in Extra-Thalamic Regions

A polyclonal, monospecific antiserum raised against a nicotinic acetylcholine receptor protein affinity-purified from insect nervous tissue, was employed to demonstrate the localization of antigenic sites in the neuropile of the terminal (sixth) abdominal ganglion of the cockroach Periplaneta americana . In agreement with previously published autoradiographic mapping of specific [ 125 I] α -bungarotoxin binding sites, specific areas of the central neuropile of this ganglion were densely stained, but not the cereal afferent axons. No staining was detected corresponding to the dense, peripheral, partly non-specific binding of α -bungarotoxin seen in autoradiographs of the same tissue. Certain peripherally located neuronal cell bodies, including the cell body of giant interneuron 2, contained intracellularly located antigenic sites.

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Nicotinic acetylcholine receptors in patients with Parkinson's disease and Alzheimer's disease: Specific binding of 2-[18F]F-A-85380 in the cerebral white matter as demonstrated by PET and comparison with diffusion tensor MRI (DTI)

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/sj.jcbfm.9591524.0584 ◽

2005 ◽

Vol 25 (1_suppl) ◽

pp. S584-S584

Author(s):

Philipp Meyer ◽

Donald Lobsien ◽

Kai Kendziorra ◽

Henryk Barthel ◽

Swen Hesse ◽

...

Keyword(s):

Alzheimer’S Disease ◽

White Matter ◽

Nicotinic Acetylcholine Receptors ◽

Acetylcholine Receptors ◽

Diffusion Tensor ◽

Specific Binding ◽

Cerebral White Matter ◽

Diffusion Tensor Mri ◽

Nicotinic Acetylcholine ◽

Disease Specific

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In Silico Analysis of Ethanol Binding Activity in Neuronal Nicotinic Acetylcholine Receptors

Malaysian Journal of Applied Sciences ◽

10.37231/myjas.2020.5.1.243 ◽

2020 ◽

Vol 5 (1) ◽

pp. 54-61

Author(s):

Angganararas Lungidningtyas ◽

Arli Aditya Parikesit

Keyword(s):

Nicotinic Acetylcholine Receptors ◽

Acetylcholine Receptors ◽

Specific Binding ◽

In Silico Analysis ◽

Data Bank ◽

Binding Activity ◽

Neuronal Nicotinic Acetylcholine Receptors ◽

Nicotinic Acetylcholine ◽

The Central Nervous System ◽

Silico Analysis

Ethanol and nicotine are two common substances that are often linked to complications in alcoholic smokers. The high number of the co-consumptions in alcoholic smokers suggested a possible interaction between ethanol and nicotine in the central nervous system and a potential similar mechanism of action. Both ethanol and nicotine are shown to bind with neuronal nicotinic acetylcholine receptors (nAChRs), a ligand gated cation channel specifically targeted by the endogenous acetylcholine. Ethanol has a much less specific binding capability to modulate the receptors, however, emerging reports indicates that ethanol can interact with nAChRs both directly and indirectly. This study focuses on the analysis of ethanol binding sites with nAChRs using molecular docking techniques obtained from the Protein Data Bank. The obtained data showed a possible binding site for ethanol in nAChRs, however, upon validation, result is not substantial. Nevertheless, the obtained data should be useful for future reference for the basis of ethanol interactions with the human nAChRs proteins.

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