Effective characterization of Salmonella Enteritidis by most probable number (MPN) followed by multiplex polymerase chain reaction (PCR) methods

2016 ◽  
Vol 24 (5) ◽  
pp. 4828-4834 ◽  
Author(s):  
Lincohn Zappelini ◽  
Solange Martone-Rocha ◽  
Milena Dropa ◽  
Maria Helena Matté ◽  
Monique Ribeiro Tiba ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Salmonella Enteritidis ◽  
Most Probable Number ◽  
Chain Reaction ◽  
Probable Number ◽  
Polymerase Chain

Application of the Polymerase Chain Reaction Technique to the Detection of Pathogens in Water

1993 ◽  
Vol 27 (3-4) ◽  
pp. 207-210 ◽  
Author(s):  
Gary A. Toranzos ◽  
Abdiel J. Alvarez ◽  
Elizabeth A. Dvorsky
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Cell ◽  
Membrane Filtration ◽  
Target Genes ◽  
Culture Media ◽  
Gene Copy ◽  
Most Probable Number ◽  
Chain Reaction ◽  
Probable Number ◽  
Polymerase Chain

Enteric pathogens may be present in fecally contaminated waters at extremely low concentrations. In addition, these pathogens may be injured when exposed to the environment and may not be able to grow in laboratory culture media or such media may simply not exist for their progagation in the laboratory. It is paramount thus to use techniques which do not depend on culture techniques for the detection of these pathogens and that allow for the detection of single-cell concentrations. The polymerase chain reaction (PCR) technique has been shown to be an excellent and sensitive means of detecting pathogens in waters. Membrane filtration has been combined with PCR and DNA hybridization techniques to be able to detect the DNA equivalent of one single cell in large volumes of water. In addition, this combination of methods allows for the amplification of different target genes fiat may be present in the sample, since the membrane can be subjected to repeated amplification reactions under different conditions. A Most Probable Number PGR was developed which allows for the quantification of gene copy number and thus permits extrapolation to estimate the number of bacterial cells in the original sample.

scholarly journals Investigating Brunei’s Seafood Markets for Vibrio parahaemolyticus using the Most Probable Number-Polymerase Chain Reaction

2013 ◽  
Vol 5 (2) ◽  
pp. 33-39 ◽  
Author(s):  
Zaidah Rizidah Murang ◽  
Siti Hanna Muharram ◽  
Oduola Olakunte Abiola
Keyword(s):  
Polymerase Chain Reaction ◽  
Vibrio Parahaemolyticus ◽  
Medical Science ◽  
Most Probable Number ◽  
Chain Reaction ◽  
Probable Number ◽  
Brunei Darussalam ◽  
High Incidence ◽  
Polymerase Chain ◽  
Very High

Background: Vibrio parahaemolyticus (V. parahaemolyticus) is the commonest source of seafood poisoning and has a very high incidence in the countries of Asian. Aims and Objectives: This study aims to investigate the presence of V. parahaemolyticus in seafood from Brunei seafood market using the Most Probable Number- Polymerase Chain Reaction (MPN-PCR). Results: None of the twnety-three seafood samples that were purchased at random from unselectively chosen wet markets and hypermarkets in Brunei Darussalam yielded V. parahaemolyticus. Conclusion: This could be due to any or a combination of: the absence or low level of V. parahaemolytius from where the samples were harvested, inactivation of V. parahaemolyticus during the processing and preservation of the samples, possible sampling factors as well as good hygienic practices in Brunei’s seafood market DOI: http://dx.doi.org/10.3126/ajms.v5i2.8948 Asian Journal of Medical Science, Volume-5(2) 2014: 33-39

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