scholarly journals Progestogen-induced alterations and their ecological relevance in different embryonic and adult behaviours of an invertebrate model species, the great pond snail (Lymnaea stagnalis)

2020 ◽  
Author(s):  
Reka Svigruha ◽  
Istvan Fodor ◽  
Judit Padisak ◽  
Zsolt Pirger
Keyword(s):  
Heart Rate ◽  
Embryonic Development ◽  
Development Time ◽  
Lymnaea Stagnalis ◽  
Feeding Activity ◽  
Future Research ◽  
Signal Pathways ◽  
Pond Snail ◽  
Model Species ◽  
Invertebrate Model

AbstractThe presence of oral contraceptives (basically applying estrogens and/or progestogens) poses a challenge to animals living in aquatic ecosystems and reflects a rapidly growing concern worldwide. However, there is still a lack in knowledge about the behavioural effects induced by progestogens on the non-target species including molluscs. In the present study, environmental progestogen concentrations were summarised. Knowing this data, we exposed a well-established invertebrate model species, the great pond snail (Lymnaea stagnalis) to relevant equi-concentrations (1, 10, 100, and 500 ng L−1) of mixtures of four progestogens (progesterone, drospirenone, gestodene, levonorgestrel) for 21 days. Significant alterations were observed in the embryonic development time, heart rate, feeding, and gliding activities of the embryos as well as in the feeding and locomotion activity of the adult specimens. All of the mixtures accelerated the embryonic development time and the gliding activity. Furthermore, the 10, 100, and 500 ng L−1 mixtures increased the heart rate and feeding activity of the embryos. The 10, 100, and 500 ng L−1 mixtures affected the feeding activity as well as the 1, 10, and 100 ng L−1 mixtures influenced the locomotion of the adult specimens. The differences of these adult behaviours showed a biphasic response to the progestogen exposure; however, they changed approximately in the opposite way. In case of feeding activity, this dose-response phenomenon can be identified as a hormesis response. Based on the authors’ best knowledge, this is the first study to investigate the non-reproductive effects of progestogens occurring also in the environment on molluscan species. Our findings contribute to the global understanding of the effects of human progestogens, as these potential disruptors can influence the behavioural activities of non-target aquatic species. Future research should aim to understand the potential mechanisms (e.g., receptors, signal pathways) of progestogens induced behavioural alterations.

scholarly journals Reversal of Age-Related Learning Deficiency by the Vertebrate PACAP and IGF-1 in a Novel Invertebrate Model of Aging: The Pond Snail (Lymnaea stagnalis)

2014 ◽  
Vol 69 (11) ◽  
pp. 1331-1338 ◽  
Author(s):  
Z. Pirger ◽  
S. Naskar ◽  
Z. Laszlo ◽  
G. Kemenes ◽  
D. Regl di ◽  
...  
Keyword(s):  
Lymnaea Stagnalis ◽  
Pond Snail ◽  
Age Related ◽  
Invertebrate Model

scholarly journals SPECIFIC FEATURES OF EMBRYONIC DEVELOPMENT OF POND SNAIL (LYMNAEA STAGNALIS L.) UNDER ANTHROPOGENIC INFLUENCE OF CHLORIDE COMPOUNDS IN WATER

2021 ◽  
Vol 2021 (3) ◽  
pp. 134-141
Author(s):  
Aleksandr Gorbunov ◽  
Dmitry Nikiforov-Nikishin ◽  
Oleg Gorbunov
Keyword(s):  
Embryonic Development ◽  
Lymnaea Stagnalis ◽  
Water Environment ◽  
Model Organism ◽  
High Sensitivity ◽  
Biological Indicators ◽  
Anthropogenic Factors ◽  
Pond Snail ◽  
Integral Parameter ◽  
Egg Shells

The article highlights the research of embryonic development of hydrobionts under the influence of anthropogenic factors of water solutions – chlorobenzene and tetrachloroethylene. To detect the toxicity of chloride pollutants in a freshwater reservoir, representatives of benthic mollusks (big pond snail Lymnaea stagnalis L.) were used as a model organism. According to the results of research, it was found that at a concentration of 0.01 mg/l of chlorobenzene solution individual anomalies of embryonic development of the pond fish are observed, but at higher concentrations its development is delayed by 5-6 stages in comparison with the control. As an integral parameter of pond fish development, we can take indicators of the biological toxicity criterion: Hatchling and its survival over a 10-day period after leaving the egg shells. Changes in the physiological parameters of pond fish embryos begin with a chlorobenzene concentration of 0.01 mg/l; the maximum permissible concentration for tetrachloroethylene for the considered biological indicators should be considered 0.05 mg/l. It is noted that in big pond snail (Lymnaea stagnalis L.) the hatch of juveniles from egg shells is the most sensitive indicator when determining the toxicity of an aqueous solution; and less sensitive indicators are the number of eggs laid and the survival of juveniles in the first 10 days after hatching. The test object used is a large pond, which has shown a high sensitivity to contamination of the water environment with chlorinated ecotoxicants and can be recommended for a comprehensive quality assessment when biotesting freshwater waters

Cholinergic interneurons in the feeding system of the pond snail Lymnaea stagnalis . III. Pharmacological dissection of the feeding rhythm

1992 ◽  
Vol 336 (1277) ◽  
pp. 181-189 ◽  
Keyword(s):  
Lymnaea Stagnalis ◽  
Feeding Activity ◽  
Reciprocal Inhibition ◽  
Experimental Results ◽  
Cholinergic Antagonists ◽  
Pond Snail ◽  
Feeding System ◽  
Inhibition Model ◽  
Cholinergic Interneurons ◽  
Feeding Rhythm

The feeding activity of the pond snail Lymnaea stagnalis was stimulated by depolarization of a modulatory interneuron (SO) or of a N1 pattern-generating interneuron. The cholinergic antagonists phenyltrim ethylammonium (PTMA), methylxylocholine (MeXCh), hexamethonium (HMT) and atropine (ATR) were applied at 0.5 mM in the bath and their effects on the rhythmic feeding pattern were monitored. Each of the antagonists slowed or blocked the feeding rhythm. The block was due to interference in the pattern generating network, not to disturbance of modulatory inputs. The experimental results favour a model in which the alternation of protraction (N l) and retraction (N2) phases occurs by recurrent inhibition. The results would be more difficult to explain on the reciprocal inhibition model. When all the N1 output was blocked, the N1 neurons fired rhythmic bursts endogenously.

scholarly journals Functional characterization and related evolutionary implications of invertebrate gonadotropin-releasing hormone/corazonin in a well-established model species

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
István Fodor ◽  
Réka Svigruha ◽  
Zsolt Bozsó ◽  
Gábor K. Tóth ◽  
Tomohiro Osugi ◽  
...  
Keyword(s):  
Lymnaea Stagnalis ◽  
Functional Characterization ◽  
Gonadotropin Releasing Hormone ◽  
Egg Laying ◽  
Pond Snail ◽  
Model Species ◽  
Releasing Hormone ◽  
Active Peptide

AbstractIn vertebrates, gonadotropin-releasing hormone (GnRH) peptide is the central mediator of reproduction. Homologous peptides have previously also been identified in molluscan species. However, emerging evidence suggests that these molecules might serve diverse regulatory functions and proposes to consider them as corazonin (CRZ). We previously isolated the full-length cDNA of the invGnRH/CRZ peptide (termed ly-GnRH/CRZ) in the well-established invertebrate model species, the great pond snail Lymnaea stagnalis; however, its predicted functions remain to be verified. In this study, we first confirmed the presence of the deduced active peptide from the central nervous system of L. stagnalis. Further, we performed in vivo and in vitro studies to explore the functions of ly-GnRH/CRZ. Injection of sexually mature specimens with synthetic active peptide had an inhibitory effect on locomotion and an acceleratory effect on egg-laying, but had no effect on feeding. The previously predicted modulatory effect of ly-GnRH/CRZ was supported by its identified co-localization with serotonin on the surface of the heart atria. Lastly, we demonstrated not only the presence of ly-GnRH/CRZ in the penial complex but also that ly-GnRH/CRZ-containing neurons project to the efferent penis nerve, suggesting ly-GnRH/CRZ may directly modulate the motor output of this peripheral tissue. Overall, our findings strongly support that ly-GnRH/CRZ is a multifunctional neuropeptide. These results contribute to the understanding of the GnRH superfamily and, more broadly, disciplines such as comparative endocrinology and neurobiology.

scholarly journals Ion channel profiling of the Lymnaea stagnalis ganglia via transcriptome analysis

2020 ◽  
Author(s):  
Nan Dong ◽  
Julia Bandura ◽  
Zhaolei Zhang ◽  
Yan Wang ◽  
Karine Labadie ◽  
...  
Keyword(s):  
Ion Channels ◽  
Lymnaea Stagnalis ◽  
De Novo ◽  
Model Organisms ◽  
Sequence Length ◽  
Future Research ◽  
Pond Snail ◽  
Sequence Information ◽  
Functional Domain ◽  
Rna Seq

Abstract Background. The pond snail Lymnaea stagnalis (L. stagnalis) has been widely used as a model organism in neurobiology, ecotoxicology, and parasitology due to the relative simplicity of its central nervous system (CNS). However, its usefulness is restricted by a limited availability of transcriptome data. While sequence information for the L. stagnalis CNS transcripts has been obtained from EST library and a de novo RNA-seq assembly, the quality of these assemblies is limited by a combination of low coverage of EST libraries, the fragmented nature of de novo assemblies, and lack of reference genome. Results. In this study, taking advantage of the recent availability of a preliminary L. stagnalis genome, we generated an RNA-seq library from the adult L. stagnalis CNS, using a combination of genome-guided and de novo assembly programs to identify 17,832 protein-coding L. stagnalis transcripts. We combined our library with existing resources to produce a transcript set with greater sequence length, completeness, and diversity than previously available ones. Using our assembly and functional domain analysis, we profiled L. stagnalis CNS transcripts encoding ion channels and ionotropic receptors, which are key proteins for CNS function, and compared their sequences to other vertebrate and invertebrate model organisms. Interestingly, L. stagnalis transcripts encoding numerous putative Ca2+ channels showed the most sequence similarity to those of Mus musculus, Danio rerio, Xenopus tropicalis, Drosophila melanogaster, and Caenorhabditis elegans, suggesting that many calcium channel-related signaling pathways may be evolutionarily conserved. Conclusions. Our study provides the most thorough characterization to date of the L. stagnalis transcriptome and provides insights into differences between vertebrates and invertebrates in CNS transcript diversity, according to function and protein class. Furthermore, this study provides a complete characterization of the ion channels of Lymnaea stagnalis, opening new avenues for future research on fundamental neurobiological processes in this model system.

scholarly journals Ion channel profiling of the Lymnaea stagnalis ganglia via transcriptome analysis

2020 ◽  
Author(s):  
Nan Dong ◽  
Julia Bandura ◽  
Zhaolei Zhang ◽  
Yan Wang ◽  
Karine Labadie ◽  
...  
Keyword(s):  
Ion Channels ◽  
Lymnaea Stagnalis ◽  
De Novo ◽  
Model Organisms ◽  
Sequence Length ◽  
Future Research ◽  
Pond Snail ◽  
Sequence Information ◽  
Functional Domain ◽  
Rna Seq

Abstract Background. The pond snail Lymnaea stagnalis (L. stagnalis) has been widely used as a model organism in neurobiology, ecotoxicology, and parasitology due to the relative simplicity of its central nervous system (CNS). However, its usefulness is restricted by a limited availability of transcriptome data. While sequence information for the L. stagnalis CNS transcripts has been obtained from EST library and a de novo RNA-seq assembly, the quality of these assemblies is limited by a combination of low coverage of EST libraries, the fragmented nature of de novo assemblies, and lack of reference genome. Results. In this study, taking advantage of the recent availability of a preliminary L. stagnalis genome, we generated an RNA-seq library from the adult L. stagnalis CNS, using a combination of genome-guided and de novo assembly programs to identify 17,832 protein-coding L. stagnalis transcripts. We combined our library with existing resources to produce a transcript set with greater sequence length, completeness, and diversity than previously available ones. Using our assembly and functional domain analysis, we profiled L. stagnalis CNS transcripts encoding ion channels and ionotropic receptors, which are key proteins for CNS function, and compared their sequences to other vertebrate and invertebrate model organisms. Interestingly, L. stagnalis transcripts encoding numerous putative Ca2+ channels showed the most sequence similarity to those of Mus musculus, Danio rerio, Xenopus tropicalis, Drosophila melanogaster, and Caenorhabditis elegans, suggesting that many calcium channel-related signaling pathways may be evolutionarily conserved. Conclusions. Our study provides the most thorough characterization to date of the L. stagnalis transcriptome and provides insights into differences between vertebrates and invertebrates in CNS transcript diversity, according to function and protein class. Furthermore, this study provides a complete characterization of the ion channels of Lymnaea stagnalis, opening new avenues for future research on fundamental neurobiological processes in this model system.

scholarly journals Ion channel profiling of the Lymnaea stagnalis ganglia via transcriptome analysis

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Nancy Dong ◽  
Julia Bandura ◽  
Zhaolei Zhang ◽  
Yan Wang ◽  
Karine Labadie ◽  
...  
Keyword(s):  
Ion Channels ◽  
Lymnaea Stagnalis ◽  
De Novo ◽  
Model Organisms ◽  
Sequence Length ◽  
Future Research ◽  
Pond Snail ◽  
Sequence Information ◽  
Functional Domain ◽  
Rna Seq

Abstract Background The pond snail Lymnaea stagnalis (L. stagnalis) has been widely used as a model organism in neurobiology, ecotoxicology, and parasitology due to the relative simplicity of its central nervous system (CNS). However, its usefulness is restricted by a limited availability of transcriptome data. While sequence information for the L. stagnalis CNS transcripts has been obtained from EST libraries and a de novo RNA-seq assembly, the quality of these assemblies is limited by a combination of low coverage of EST libraries, the fragmented nature of de novo assemblies, and lack of reference genome. Results In this study, taking advantage of the recent availability of a preliminary L. stagnalis genome, we generated an RNA-seq library from the adult L. stagnalis CNS, using a combination of genome-guided and de novo assembly programs to identify 17,832 protein-coding L. stagnalis transcripts. We combined our library with existing resources to produce a transcript set with greater sequence length, completeness, and diversity than previously available ones. Using our assembly and functional domain analysis, we profiled L. stagnalis CNS transcripts encoding ion channels and ionotropic receptors, which are key proteins for CNS function, and compared their sequences to other vertebrate and invertebrate model organisms. Interestingly, L. stagnalis transcripts encoding numerous putative Ca2+ channels showed the most sequence similarity to those of Mus musculus, Danio rerio, Xenopus tropicalis, Drosophila melanogaster, and Caenorhabditis elegans, suggesting that many calcium channel-related signaling pathways may be evolutionarily conserved. Conclusions Our study provides the most thorough characterization to date of the L. stagnalis transcriptome and provides insights into differences between vertebrates and invertebrates in CNS transcript diversity, according to function and protein class. Furthermore, this study provides a complete characterization of the ion channels of Lymnaea stagnalis, opening new avenues for future research on fundamental neurobiological processes in this model system.

Sign in / Sign up

Export Citation Format

Share Document