Embryotoxic effects of tribromophenol on early post-implantation development of mouse embryos in vitro

2021 ◽  
Author(s):  
Zhihua Zhao ◽  
Dicong Zhu ◽  
Yujie Liu ◽  
Qing Zhou ◽  
Jingfan Qiu ◽  
...  
Keyword(s):  
Mouse Embryos ◽  
Embryotoxic Effects ◽  
Post Implantation

Pregnancy: Effect of pentoxifylline on implantation and post-implantation development of mouse embryos in vitro

1993 ◽  
Vol 8 (11) ◽  
pp. 1948-1954 ◽  
Author(s):  
Herman Tournaye ◽  
Marleen Van der Linden ◽  
Etienne Van den Abbeel ◽  
Paul Devroey ◽  
André Van Steirteghem
Keyword(s):  
Mouse Embryos ◽  
Post Implantation

scholarly journals Effect of retinoic acid on implantation and post-implantation development of mouse embryos in vitro

2001 ◽  
Vol 16 (10) ◽  
pp. 2171-2176 ◽  
Author(s):  
Fu-Jen Huang ◽  
Tsung-Chieh J. Wu ◽  
Meng-Yin Tsai
Keyword(s):  
Retinoic Acid ◽  
Mouse Embryos ◽  
Post Implantation

scholarly journals ­­Highly Conducive Ex utero Mouse Embryogenesis from Pre-Gastrulation to Late Organogenesis

2021 ◽  
Author(s):  
Alejandro Aguilera-Castrejon ◽  
Jacob H. Hanna
Keyword(s):  
Mouse Embryo ◽  
Embryo Culture ◽  
Hind Limb ◽  
Normal Mouse ◽  
Mouse Embryos ◽  
Formation Stage ◽  
Stable Growth ◽  
Post Implantation ◽  
Mouse Embryo Culture

Abstract In mammals, morphogenesis and organogenesis take place after the embryo implants into the uterus, which makes it relatively inaccessible for observation and manipulation. While methods for in vitro culture of pre- and peri-implantation mouse embryos are routinely used, approaches for efficient and stable culture of post-implantation embryos from egg cylinder stages until advanced organogenesis remain to be established. We recently developed highly robust ex utero post-implantation mouse embryo culture platforms, that enable appropriate and faithful development of embryos before gastrulation (E5.5) until the hind limb formation stage (E11). In these protocols, late gastrulating embryos (E7.5) are grown in 3D rotating bottles settings, while extended culture from pre-gastrulation stages (E5.5 or E6.5) requires a combination of static and rotating bottle culture protocols. These systems support stable growth of normal mouse embryos ex utero from pre-gastrulation to advanced organogenesis.

scholarly journals Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marino Maemura ◽  
Hiroaki Taketsuru ◽  
Yuki Nakajima ◽  
Ruiqi Shao ◽  
Ayaka Kakihara ◽  
...  
Keyword(s):  
Inner Cell Mass ◽  
Cell Mass ◽  
Mouse Embryos ◽  
Primitive Endoderm ◽  
Cell Stage ◽  
Supportive Environment ◽  
Mouse Zygotes ◽  
Single Blastomeres ◽  
Multicellular Organisms

AbstractIn multicellular organisms, oocytes and sperm undergo fusion during fertilization and the resulting zygote gives rise to a new individual. The ability of zygotes to produce a fully formed individual from a single cell when placed in a supportive environment is known as totipotency. Given that totipotent cells are the source of all multicellular organisms, a better understanding of totipotency may have a wide-ranging impact on biology. The precise delineation of totipotent cells in mammals has remained elusive, however, although zygotes and single blastomeres of embryos at the two-cell stage have been thought to be the only totipotent cells in mice. We now show that a single blastomere of two- or four-cell mouse embryos can give rise to a fertile adult when placed in a uterus, even though blastomere isolation disturbs the transcriptome of derived embryos. Single blastomeres isolated from embryos at the eight-cell or morula stages and cultured in vitro manifested pronounced defects in the formation of epiblast and primitive endoderm by the inner cell mass and in the development of blastocysts, respectively. Our results thus indicate that totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage.

Prolonged in vitro precultivation alleviates post-implantation inflammation and promotes stable subcutaneous cartilage formation in a goat model

2016 ◽  
Vol 12 (1) ◽  
pp. 015006 ◽  
Author(s):  
Yi Liu ◽  
Dan Li ◽  
Zongqi Yin ◽  
Xusong Luo ◽  
Wei Liu ◽  
...  
Keyword(s):  
Cartilage Formation ◽  
Post Implantation
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