Correction to: Ganga river water quality assessment using combined approaches: physico-chemical parameters and cyanobacterial toxicity detection with special reference to microcystins and molecular characterization of microcystin synthetase (mcy) genes carrying cyanobacteria

Abstract Water quality assessment relies mostly on physico-chemical based characterization however, eutrophication and climate change advocate the abundance of toxic microcystins (MCs) producing cyanobacteria as emerging bio-indicator. In the present study, a spatial-temporal analysis was carried out at ten sampling sites of Prayagraj and Varanasi during June 2017 and March 2018 to determine the Ganga River water quality using physico-chemical parameters, cyanobacteria diversity, detection of MCs producing strains and MC-LR equivalence. Coliform bacteria, COD, NO3-N and phosphate are the significant contaminated parameters favoring the growth of putative MCs producing cyanobacteria. National Sanitation Foundation WQI (NSFWQI) indicates water quality, either bad or medium category at sampling points. The morphological analysis confirms the occurrence of diverse cyanobacterial genera such as Microcystis, Anabaena, Oscillatoria, Phormidium etc. PCR amplification affirmed the presence of toxic microcystin (mcy) genes in uncultured cyanobacteria at all the sampling sites. The concentration of MC-LR equivalence in water samples by protein phosphatase 1 inhibition assay (PPIA) and high-performance liquid chromatography (HPLC) methods was observed in the range of 23.4–172 ng/L and 13.2–97.5 ng/L respectively which is lower than the harmful exposure limit by World Health Organization (WHO). Ganga isolate 1 was identified as Microcystis based on partial 16S rDNA sequence and its toxicity was confirmed due to presence of mcy genes and MCs production potential. These findings suggest the presence of MCs producers as new emerging parameter to monitor water quality index and identification up to species level will be valuable for restoration strategies of river Ganga.

Download Full-text

Physico-chemical attributes of water of Hakaluki Haor, Sylhet, Bangladesh

Jahangirnagar University Journal of Biological Sciences ◽

10.3329/jujbs.v3i2.28287 ◽

2016 ◽

Vol 3 (2) ◽

pp. 67-72

Author(s):

Md Tajul Islam ◽

M Mahfuzur Rahman ◽

Hassan Mahmud

Keyword(s):

Water Quality ◽

Seasonal Variation ◽

Water Samples ◽

Water Quality Assessment ◽

Inorganic Pollutants ◽

Chemical Parameters ◽

Ph Values ◽

Physico Chemical ◽

Significant Seasonal Variation ◽

Chemical Attributes

Water quality assessment has been carried out in the Hakaluki Haor of greater Sylhet district during May, 2013 to February, 2014. Water samples were collected from 5 spots during 4 seasons and a total of 15 physico-chemical parameters were studied. Significant seasonal variation was observed in all seasons among physico-chemical parameters. pH values varied from 5.9 - 7.1, water temperature varied from 23.7 26.7OC, transference varied from 23-53 cm, TSS varied from 18.1-192 mg/l, TDS varied 25-193.5 mg/l, EC varied from 30-680 ?s/cm , DO content of water varied from 4.6 - 5.6mg/l, BOD varied from 0.2-2.614 mg/l, COD varied from 38-312 mg/l, NO3-N varied from 0.1-30.4 mg/l, NH3 varied from 0.9-2.18 mg/l, alkalinity varied from 12-42 mg/l and PO4 varied from 0.9 -13.4 (mg/l). Agricultural and inorganic pollutants have been observed as the main causes of the Haor water pollution.Jahangirnagar University J. Biol. Sci. 3(2): 67-72, 2014 (December)

Download Full-text