Genetic diversity in Chinese modern wheat varieties revealed by microsatellite markers

Wheat (Triticum aestivum L.) is the most important and strategic cereal crop in Egypt and has many bread wheat varieties. Seventeen Egyptian bread wheat varieties used in this study with a set of sixteen wheat microsatellite markers to examine their utility in detecting DNA polymorphism, estimating genetic diversity and identifying genotypes. A total of 190 alleles were detected at 16 loci using 16 microsatellite primer pairs. The number of allele per locus ranged from 8 to 20, with an average of 11.875. The polymorphic information content (PIC) and marker index (MI) average values were 0.8669, 0.8530 respectively. The (GA) n microsatellites were the highest polymorphic (20 alleles). The Jaccard's Coefficient for genetic similarity was ranged from 0.524 to 0.109 with average of 0.375. A dendrogram was prepared based on similarity matrix using UPGMA algorithm, divided the cultivars into two major clusters. The results proved the microsatellite markers utility in detecting polymorphism due to the discrimination of cultivars and estimating genetic diversity.

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Genetic diversity analysis among pre-green revolution, post-green revolution era cultivars, and wheat landraces as revealed by microsatellite markers

Crop and Pasture Science ◽

10.1071/cp08310 ◽

2009 ◽

Vol 60 (4) ◽

pp. 373 ◽

Cited By ~ 4

Author(s):

Neelu Jain ◽

Rajbir Yadav

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Bread Wheat ◽

Genetic Relationships ◽

Green Revolution ◽

Triticum Aestivum L ◽

Yield Potential ◽

Wheat Varieties ◽

High Yielding Varieties ◽

Novel Alleles

Bread wheat (Triticum aestivum L.) is the most widely grown crop in the world, and India is the second largest wheat producer after China. Introduction of input-responsive, semi-dwarf varieties set the foundation for the green revolution in the mid-1960s. To meet the future challenge of increasing food production with a shrinking land base, new varieties with higher yield potential and increased yield stability have to be developed by using the diverse genetic resource. The objective of this study was to evaluate genetic diversity in 74 wheat genotypes including released varieties in India occupying the pre-green revolution period (before 1965) and post-green revolution period (after 1965) and land races with microsatellite markers. SSRs represent a powerful tool to quantify genetic diversity in wheat. In total, 170 alleles were detected with an average of 3.3 alleles per locus. Overall, 24 rare alleles were present and 11 unique alleles were found in the studied landraces only. A positive correlation was found between the number of alleles and genetic diversity. Genetic relationships as determined by UPGMA (NTSYS-pc) and structure analyses grouped all modern wheat cultivars under one node. The traditional tall varieties released during the pre-green revolution era were clustered along with some of the landraces, indicating that they had possibly been developed through selection among the landraces. Diversity among the released varieties in the post-green revolution era has widened rather than narrowing down. Molecular variance analysis showed that variance was mainly distributed within (91.9%) rather than among (8.01%) the bread wheat varieties and landraces. The diversity obtained within the landraces proves them to be an important reservoir of biodiversity and source of novel alleles for use in breeding programs. Landraces such as MPG 62 and MPG 82 can be used for introgressing rare and unique alleles in the genetic background of high-yielding varieties.

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Genetic diversity based on microsatellite markers in five Nile tilapia strains

Biodiversity Science ◽

10.3724/sp.j.1003.2009.08266 ◽

2009 ◽

Vol 17 (1) ◽

pp. 82 ◽

Cited By ~ 2

Author(s):

Liang Hongwei ◽

Li Zhong ◽

Luo Xiangzhong ◽

Wang Changzhong ◽

Hu Guangfu ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Nile Tilapia

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Revealing the genetic diversity of wheat varieties (lines) in China based on SNP markers

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2020.91039 ◽

2019 ◽

Vol 46 (2) ◽

pp. 307-314

Author(s):

Yu-Qing ZHANG ◽

Juan ZOU ◽

Yi-Ke LIU ◽

Wei-Jie HE ◽

Zhan-Wang ZHU ◽

...

Keyword(s):

Genetic Diversity ◽

Snp Markers ◽

Wheat Varieties

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First Records on Genetic Diversity and Population Structure of Algerian Peanut (Arachis hypogaea) Using Microsatellite Markers

Plant Molecular Biology Reporter ◽

10.1007/s11105-021-01305-7 ◽

2021 ◽

Author(s):

Hanène Djeghim ◽

Ines Bellil ◽

Rima Hind Boudchicha ◽

Ali Boumegoura ◽

Douadi Khelifi

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Microsatellite Markers ◽

Arachis Hypogaea

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Genetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite markers

Heredity ◽

10.1046/j.1365-2540.2001.00852.x ◽

2001 ◽

Vol 86 (4) ◽

pp. 469-479 ◽

Cited By ~ 97

Author(s):

Stéphanie Mariette ◽

David Chagné ◽

Céline Lézier ◽

Patrick Pastuszka ◽

Annie Raffin ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Pinus Pinaster

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Genetic diversity and population structure of date palms (Phoenix dactylifera L.) in Ethiopia using microsatellite markers

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-021-00168-5 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Workia Ahmed ◽

Tileye Feyissa ◽

Kassahun Tesfaye ◽

Sumaira Farrakh

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Microsatellite Markers ◽

Information Content ◽

Date Palm ◽

Polymorphic Information Content ◽

Phoenix Dactylifera ◽

Mean Value ◽

Date Palms ◽

Expected Heterozygosity

Abstract Background Date palm tree (Phoenix dactylifera L.) is a perennial monocotyledonous plant belonging to the Arecaceae family, a special plant with extraordinary nature that gives eminent contributions in agricultural sustainability and huge socio-economic value in many countries of the world including Ethiopia. Evaluation of genetic diversity across date palms at DNA level is very important for breeding and conservation. The result of this study could help to design for genetic improvement and develop germplasm introduction programmes of date palms mainly in Ethiopia. Results In this study, 124 date palm genotypes were collected, and 10 polymorphic microsatellite markers were used. Among 10 microsatellites, MPdCIR085 and MPdCIR093 loci showed the highest value of observed and expected heterozygosity, maximum number of alleles, and highest polymorphic information content values. A total of 112 number of alleles were found, and the mean number of major allele frequency was 0.26, with numbers ranging from 0.155 (MPdCIR085) to 0.374 (MPdCIR016); effective number of alleles with a mean value of 6.61, private alleles ranged from 0.0 to 0.65; observed heterozygosity ranged from 0.355 to 0.726; expected heterozygosity varied from 0.669 to 0.906, polymorphic information content with a mean value of 0.809; fixation index individuals relative to subpopulations ranged from 0.028 for locus MPdCIR032 to 0.548 for locus MPdCIR025, while subpopulations relative to total population value ranged from − 0.007 (MPdCIR070) to 0.891 (MPdCIR015). All nine accesstions, neighbour-joining clustering analysis, based on dissimilarity coefficient values were grouped into five major categories; in population STRUCTURE analysis at highest K value, three groups were formed, whereas DAPC separated date palm genotypes into eight clusters using the first two linear discriminants. Principal coordinate analysis was explained, with a 17.33% total of variation in all populations. Generally, the result of this study revealed the presence of allele variations and high heterozygosity (> 0.7) in date palm genotypes. Conclusions Microsatellites (SSR) are one of the most preferable molecular markers for the study of genetic diversity and population structure of plants. In this study, we found the presence of genetic variations of date palm genotypes in Ethiopia; therefore, these genetic variations of date palms is important for crop improvement and conservation programmes; also, it will be used as sources of information to national and international genbanks.

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