scholarly journals Antisense expression of Gossypium barbadense UGD6 in Arabidopsis thaliana significantly alters cell wall composition

2016 ◽  
Vol 59 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Jie Han ◽  
Yuxin Pan ◽  
Xingfen Wang ◽  
Yan Zhang ◽  
Zhiying Ma
Author(s):  
Margalida Roig‐Oliver ◽  
Catherine Rayon ◽  
Romain Roulard ◽  
François Fournet ◽  
Josefina Bota ◽  
...  

2010 ◽  
Vol 64 (4) ◽  
pp. 679-686 ◽  
Author(s):  
Yun-Kuan Liang ◽  
Xiaodong Xie ◽  
Shona E. Lindsay ◽  
Yi Bing Wang ◽  
Josette Masle ◽  
...  

2013 ◽  
Vol 6 (1) ◽  
pp. 46 ◽  
Author(s):  
Rebecca Van Acker ◽  
Ruben Vanholme ◽  
Véronique Storme ◽  
Jennifer C Mortimer ◽  
Paul Dupree ◽  
...  

2007 ◽  
Vol 98 (16) ◽  
pp. 2985-2992 ◽  
Author(s):  
Gautam Sarath ◽  
Lisa M. Baird ◽  
Kenneth P. Vogel ◽  
Robert B. Mitchell

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Veronica Giourieva ◽  
Emmanuel Panteris

Abstract Background Cortical microtubules regulate cell expansion by determining cellulose microfibril orientation in the root apex of Arabidopsis thaliana. While the regulation of cell wall properties by cortical microtubules is well studied, the data on the influence of cell wall to cortical microtubule organization and stability remain scarce. Studies on cellulose biosynthesis mutants revealed that cortical microtubules depend on Cellulose Synthase A (CESA) function and/or cell expansion. Furthermore, it has been reported that cortical microtubules in cellulose-deficient mutants are hypersensitive to oryzalin. In this work, the persistence of cortical microtubules against anti-microtubule treatment was thoroughly studied in the roots of several cesa mutants, namely thanatos, mre1, any1, prc1-1 and rsw1, and the Cellulose Synthase Interacting 1 protein (csi1) mutant pom2-4. In addition, various treatments with drugs affecting cell expansion were performed on wild-type roots. Whole mount tubulin immunolabeling was applied in the above roots and observations were performed by confocal microscopy. Results Cortical microtubules in all mutants showed statistically significant increased persistence against anti-microtubule drugs, compared to those of the wild-type. Furthermore, to examine if the enhanced stability of cortical microtubules was due to reduced cellulose biosynthesis or to suppression of cell expansion, treatments of wild-type roots with 2,6-dichlorobenzonitrile (DCB) and Congo red were performed. After these treatments, cortical microtubules appeared more resistant to oryzalin, than in the control. Conclusions According to these findings, it may be concluded that inhibition of cell expansion, irrespective of the cause, results in increased microtubule stability in A. thaliana root. In addition, cell expansion does not only rely on cortical microtubule orientation but also plays a regulatory role in microtubule dynamics, as well. Various hypotheses may explain the increased cortical microtubule stability under decreased cell expansion such as the role of cell wall sensors and the presence of less dynamic cortical microtubules.


Author(s):  
Eliza Louback ◽  
Diego Silva Batista ◽  
Tiago Augusto Rodrigues Pereira ◽  
Talita Cristina Mamedes-Rodrigues ◽  
Tatiane Dulcineia Silva ◽  
...  

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