Identification and red blood cell automated counting from blood smear images using computer-aided system

2017 ◽  
Vol 56 (3) ◽  
pp. 483-489 ◽  
Author(s):  
Vasundhara Acharya ◽  
Preetham Kumar
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Smear ◽  
Computer Aided

scholarly journals Computer Aided System for Red Blood Cell Classification in Blood Smear Image

2014 ◽  
Vol 42 ◽  
pp. 206-213 ◽  
Author(s):  
Razali Tomari ◽  
Wan Nurshazwani Wan Zakaria ◽  
Muhammad Mahadi Abdul Jamil ◽  
Faridah Mohd Nor ◽  
Nik Farhan Nik Fuad
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Smear ◽  
Cell Classification ◽  
Computer Aided ◽  
Blood Smear Image

scholarly journals Red blood cell classification on thin blood smear images for malaria diagnosis

2020 ◽  
Vol 1444 ◽  
pp. 012036 ◽  
Author(s):  
Budi Sunarko ◽  
Djuniadi ◽  
Murk Bottema ◽  
Nur Iksan ◽  
Khakim A N Hudaya ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Smear ◽  
Malaria Diagnosis ◽  
Thin Blood Smear ◽  
Cell Classification

scholarly journals COLOR INFORMATION IN BLOOD CELLS

1974 ◽  
Vol 22 (7) ◽  
pp. 517-521 ◽  
Author(s):  
GEORGE C. CHENG
Keyword(s):  
Blood Cell ◽  
Prior Knowledge ◽  
Light Source ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Blood Smear ◽  
Giemsa Stain ◽  
Color Information ◽  
Color Spectrum ◽  
Reference Samples

Two hundred color spectra of blood cells treated with Wright-Giemsa stain were measured by using an Olympus microspectrophotometer. The wavelength of the light source was varied from 400 to 700 nm. Several basic pictorial regions in the blood smear were investigated. These regions included the background, basophil, eosinophil, neutrophil, lymphocyte, monocyte and the red blood cell. A subset of the color spectrum curves was used as references. The remaining spectrum curves were used as testing samples and were shown to individuals who had no prior knowledge of leukocyte color information. All of the persons tested could correctly identify the testing samples with the reference samples. This experiment showed that color information in blood cells is not only useful but also sufficient for leukocyte differentiation for the samples that have been investigated.

scholarly journals Hematological and biochemical profile of person with macrocytic anemia in a tertiary health care centre of Nepal

2020 ◽  
Vol 10 (2) ◽  
pp. 1722-1727
Author(s):  
Rakesh Pathak ◽  
Sujata Pudasaini ◽  
Sushmita Ghimire ◽  
Anil Singh Basnyat ◽  
Anuj KC
Keyword(s):  
Folic Acid ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Hematological Parameters ◽  
Macrocytic Anemia ◽  
Peripheral Blood Smear ◽  
Blood Smear Examination ◽  
Red Blood Cell Indices

Background: Anemia is a nutritional problem worldwide with an increased risk of morbidity and mortality in all age groups. Macrocytic anemia often originates from abnormalities that impair the erythroid precursor maturation in the bone marrow. Since the clinical manifestations of different types of anemias are similar, hematological parameters including hemoglobin, Red blood cell indices, and Peripheral Blood Smear examination are useful in the diagnosis of anemia. Materials and Methods: This was a cross-sectional study done in the Department of Pathology at Nepal Medical College Teaching hospital. A total of 42 patients between 14 to 62 years with low Hb concentration according to the World Health Organization criteria for anemia were selected and a mean cell volume > 100 fL was taken for study. Peripheral blood smear examination, Red blood cell indices, Vitamin B12, and Folic acid level were evaluated. Results: There were 42 patients with macrocytic anemia enrolled in the study with a mean age of 31.85±12.49 years and with female preponderance. Hemoglobin level was slightly low in males compared to females. Red blood cell indices were slightly higher in males. The difference of serum Vit B12 and Folic acid in male and female was found to be significant. Conclusions: It was concluded that for the diagnosis of a specific type of anemia, hemoglobin, Red blood cell indices, reticulocytes percent, and PBS examination were important parameters. Serum Folic acid and Vitamin B12 level estimation along with other hematological parameters are important for the diagnosis of macrocytic anemia and its correlation

Flagging performance of Sysmex XN-10 haematology analyser for malaria detection

2020 ◽  
Vol 73 (10) ◽  
pp. 676-677
Author(s):  
Cécile Dumas ◽  
Pauline Tirard-Collet ◽  
Fanélie Mestrallet ◽  
Sandrine Girard ◽  
Laurent Jallades ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Smear ◽  
Rapid Review ◽  
Predictive Values ◽  
Blood Samples ◽  
Haematology Analyser ◽  
Sensitivity Specificity ◽  
Low Prevalence

AimThe aim was to assess the flagging performance of Sysmex XN-10 haematology analyser for malaria detection through the parasitic red blood cell (‘pRBC’) alarm.MethodsWe retrospectively studied 584 blood samples performed on the Sysmex XN-10 analyser that were tested for malaria. Sensitivity, specificity, positive and negative predictive values, and prevalence were established for the pRBC alarm.ResultsSensitivity, specificity, and positive and negative predictive values for the pRBC flag were 7.8%, 100%, 100% and 87.7%, respectively. The prevalence of pRBC flag of 0.026% in the overall population was significantly different from the prevalence of 1.027% in the population tested for malaria.ConclusionsConsidering the excellent specificity and the low prevalence of the flag in the overall population, we suggest, in case of the presence of pRBC flag, the implementation of a rapid review of the blood smear looking for Plasmodium, mostly if the patient had fever and had not been tested for malaria.

scholarly journals Evaluation of frequency and intensity of asymptomatic anisocytosis in the Japanese dog breeds Shiba, Akita, and Hokkaido

2017 ◽  
Vol 86 (4) ◽  
pp. 385-391
Author(s):  
Olga Aniołek ◽  
Agnieszka Barc ◽  
Anna Jarosińska ◽  
Zdzisław Gajewski
Keyword(s):  
Blood Cell ◽  
Cell Volume ◽  
Red Blood Cell ◽  
Blood Smear ◽  
Haemoglobin Concentration ◽  
Cell Distribution ◽  
Preventative Care ◽  
Mean Cell Volume ◽  
Distribution Width ◽  
Red Blood Cell Distribution

Microcytosis is observed in healthy Japanese breed dogs. The aim of the study was to evaluate the frequency and intensity of asymptomatic anisocytosis using a three-grade scale in Japanese dog breeds with special emphasis on the following indices: mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, and red blood cell distribution width. The retrospective study included analyses of blood morphology and blood smear for clinically healthy Japanese dog breeds Shiba, Akita, and Hokkaido aged from 6 months to 14 years, performed as a part of preventative care. A total of 74 dogs of both sexes were qualified for the study. The group included both neutered and non-neutered animals (Akita – 17 females, 12 males, Shiba – 24 females, 18 males, Hokkaido – 2 females, 1 male). The blood smear revealed significant anisocytosis in 60.8% and mild anisocytosis in 28.4% of the tested dogs – 89.2% in total. Microcytosis was reported for 25.7% of the tested Japanese breed dogs. Reduced mean cell haemoglobin and mean cell haemoglobin concentration were diagnosed in 75.7% and 40.5% of dogs, respectively. Red blood cell distribution width as an anisocytosis indicator exceeded the norm in 12% of the tested dogs. Compared to mixed breed dogs, the Japanese breeds had a reduced mean cell volume, mean cell haemoglobin concentration and significant anisocytosis in the blood smear as well as a higher red blood cell distribution width indicator. Veterinarians should consider these differences when interpreting the results of morphological blood tests.

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