Flagging performance of Sysmex XN-10 haematology analyser for malaria detection

2020 ◽  
Vol 73 (10) ◽  
pp. 676-677
Author(s):  
Cécile Dumas ◽  
Pauline Tirard-Collet ◽  
Fanélie Mestrallet ◽  
Sandrine Girard ◽  
Laurent Jallades ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Smear ◽  
Rapid Review ◽  
Predictive Values ◽  
Blood Samples ◽  
Haematology Analyser ◽  
Sensitivity Specificity ◽  
Low Prevalence

AimThe aim was to assess the flagging performance of Sysmex XN-10 haematology analyser for malaria detection through the parasitic red blood cell (‘pRBC’) alarm.MethodsWe retrospectively studied 584 blood samples performed on the Sysmex XN-10 analyser that were tested for malaria. Sensitivity, specificity, positive and negative predictive values, and prevalence were established for the pRBC alarm.ResultsSensitivity, specificity, and positive and negative predictive values for the pRBC flag were 7.8%, 100%, 100% and 87.7%, respectively. The prevalence of pRBC flag of 0.026% in the overall population was significantly different from the prevalence of 1.027% in the population tested for malaria.ConclusionsConsidering the excellent specificity and the low prevalence of the flag in the overall population, we suggest, in case of the presence of pRBC flag, the implementation of a rapid review of the blood smear looking for Plasmodium, mostly if the patient had fever and had not been tested for malaria.

Real-time Red Blood Cell Counting and Osmolarity Analysis using a Photoacoustic-based Microfluidic System

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Wenxiu Zhao ◽  
Haibo Yu ◽  
Yangdong Wen ◽  
Hao Luo ◽  
Boliang Jia ◽  
...  
Keyword(s):  
Blood Cell ◽  
Physical Properties ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Diagnostic Procedure ◽  
Microfluidic System ◽  
Cell Counting ◽  
Blood Samples ◽  
System P ◽  
Blood Cell Counting

Counting the number of red blood cells (RBCs) in blood samples is a common clinical diagnostic procedure, but conventional methods are unable to provide the size and other physical properties...

In vitro effects of temperature on red blood cell deformability and membrane stability in human and various vertebrate species

2021 ◽  
pp. 1-10
Author(s):  
Adam Attila Matrai ◽  
Gabor Varga ◽  
Bence Tanczos ◽  
Barbara Barath ◽  
Adam Varga ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Whole Blood ◽  
Mechanical Stability ◽  
Cell Deformability ◽  
Blood Samples ◽  
Effects Of Temperature ◽  
The Way ◽  
Rbc Deformability

BACKGROUND: The effects of temperature on micro-rheological variables have not been completely revealed yet. OBJECTIVE: To investigate micro-rheological effects of heat treatment in human, rat, dog, and porcine blood samples. METHODS: Red blood cell (RBC) - buffer suspensions were prepared and immersed in a 37, 40, and 43°C heat-controlled water bath for 10 minutes. Deformability, as well as mechanical stability of RBCs were measured in ektacytometer. These tests were also examined in whole blood samples at various temperatures, gradually between 37 and 45°C in the ektacytometer. RESULTS: RBC deformability significantly worsened in the samples treated at 40 and 43°C degrees, more expressed in human, porcine, rat, and in smaller degree in canine samples. The way of heating (incubation vs. ektacytometer temperation) and the composition of the sample (RBC-PBS suspension or whole blood) resulted in the different magnitude of RBC deformability deterioration. Heating affected RBC membrane (mechanical) stability, showing controversial alterations. CONCLUSION: Significant changes occur in RBC deformability by increasing temperature, showing inter-species differences. The magnitude of alterations is depending on the way of heating and the composition of the sample. The results may contribute to better understanding the micro-rheological deterioration in hyperthermia or fever.

scholarly journals Analysis of the Relationships between Mean Red Blood Cell Volume, Red Blood Cell Distribution Width and Hypotension in Patients with Hemodialysis

2021 ◽  
Author(s):  
Jiayong Xie ◽  
Qiang Zhu ◽  
Yuqi Qian ◽  
Gang Yao ◽  
Ying Yuan
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Antihypertensive Drugs ◽  
Characteristic Curve ◽  
Maintenance Hemodialysis ◽  
Cell Distribution ◽  
Distribution Width ◽  
Ultra Filtration ◽  
Sensitivity Specificity ◽  
Red Blood Cell Distribution

Background: We aimed to explore and analyze the relationships between mean corpuscular volume (MCV), red blood cell distribution width (RDW) and hypotension in patients with hemodialysis. Methods: The clinical data of 163 patients from the Xinghua People’s Hospital, Taizhou, China with hemodialysis were retrospectively analyzed. The incidence of hypotension was counted and the levels of MCV and RDW were compared between the patients with and without hemodialysis. MCV and RDW were analyzed as possible influencing factors of hypotension. Receiver operating characteristic curve (ROC) was drawn to analyze the effect of MCV and RDW on the risk assessment of hypotension in patients with hemodialysis. Results: MCV in patients with hypotension was significantly lower than those without hypotension (P < 0.05), and RDW was higher than those without hypotension (P < 0.05). The constituent ratio of higher age (>60), diabetic nephropathy, maintenance hemodialysis, MCV < 80fl, RDW > 14.8%, malnutrition, anemia, ultra-filtration rate, diet during dialysis, coronary heart disease, atrial fibrillation and antihypertensive drugs before dialysis were higher in patients with hypotension than those without hypotension (P < 0.05). The sensitivity, specificity and AUC of the combination of MCV and RDW were higher than those of the single assessment. MCV is lower in patients with hypotension and RDW is higher than those in patients without hypotension. Conclusion: MCV combined with RDW has a good evaluation effect.

scholarly journals Red blood cell classification on thin blood smear images for malaria diagnosis

2020 ◽  
Vol 1444 ◽  
pp. 012036 ◽  
Author(s):  
Budi Sunarko ◽  
Djuniadi ◽  
Murk Bottema ◽  
Nur Iksan ◽  
Khakim A N Hudaya ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Smear ◽  
Malaria Diagnosis ◽  
Thin Blood Smear ◽  
Cell Classification

scholarly journals COLOR INFORMATION IN BLOOD CELLS

1974 ◽  
Vol 22 (7) ◽  
pp. 517-521 ◽  
Author(s):  
GEORGE C. CHENG
Keyword(s):  
Blood Cell ◽  
Prior Knowledge ◽  
Light Source ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Blood Smear ◽  
Giemsa Stain ◽  
Color Information ◽  
Color Spectrum ◽  
Reference Samples

Two hundred color spectra of blood cells treated with Wright-Giemsa stain were measured by using an Olympus microspectrophotometer. The wavelength of the light source was varied from 400 to 700 nm. Several basic pictorial regions in the blood smear were investigated. These regions included the background, basophil, eosinophil, neutrophil, lymphocyte, monocyte and the red blood cell. A subset of the color spectrum curves was used as references. The remaining spectrum curves were used as testing samples and were shown to individuals who had no prior knowledge of leukocyte color information. All of the persons tested could correctly identify the testing samples with the reference samples. This experiment showed that color information in blood cells is not only useful but also sufficient for leukocyte differentiation for the samples that have been investigated.

scholarly journals Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Dietmar Enko ◽  
Claudia Habres ◽  
Franz Wallner ◽  
Barbara Mayr ◽  
Gabriele Halwachs-Baumann
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Samples ◽  
Antibody Screening ◽  
Indirect Antiglobulin Test ◽  
Cell Antibody ◽  
Antiglobulin Test ◽  
Low Ionic Strength ◽  
Enzyme Test ◽  
Screening Result

The aim of this study was to determine the frequencies and specificities of “enzyme-only” detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria. Routine blood samples of 2420 patients were investigated. The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LISS-) indirect antiglobulin test (IAT) and with an enzyme-pretreated (papain) 3-cell panel fully automated on the ORTHO AutoVue Innova System. The antibody identification was carried out manually with an 11-cell panel in the LISS-IAT and with an enzyme-pretreated (papain) 11-cell panel. In total 4.05% (n=98) of all patients (n=2420) had a positive RBC antibody screening result. Of them 25.51% (25/98) showed “enzyme-only” detected specific or nonspecific RBC alloantibodies. Rhesus and Lewis system antibodies were found the only specificities of “enzyme-only” RBC alloantibodies: all in all 4.8% (4/98) were detected with anti-E, 3.06% (3/98) with anti-Lea, 3.06% (3/98) with anti-D after anti-D prophylaxis and 1.02% (1/98) with anti-e. In total, 14.29% (14/98) showed a nonspecific RBC alloantibody result with the enzyme test. The results of the present study demonstrate that a high number of unwanted positive reactions with the enzyme technique overshadows the detection of “enzyme-only” RBC alloantibodies. (Trial Registration: K-37-13).

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