Accuracy and precision of non-invasive continuous haemoglobin concentration monitoring in diabetic patients

Background Accuracy and precision of non-invasive continuous haemoglobin concentration (SpHb) provided by Masimo device in diabetic patients is poorly studied. This retrospective analysis aimed to provide data on SpHb accuracy and precision in diabetic patients. Results The sample size population consisted of 14 patients, with 56 SpHb/Lab data pairs. Lab value showed a mean ± standard deviation (SD) of 13.2 ± 1.2 g/dL, whilst SpHb showed a mean ± SD of 11.8 ± 1.1 g/dL. Linear regression analysis between Lab/SpHb data pairs showed a r of 0.8960 (CI95% 0.8281-0.9379, p value < 0.0001). SpHb underestimated the real Hb values provided by Lab. Bland-Altman analysis showed that SpHb accuracy was −1.37 g/dL (CI95% −1.51 to −1.22 g/dL, p value < 0.0001), precision of 0.55 g/dL, lower LOA −2.45 g/dL (CI95% −2.71 to −2.20 g/dL) and upper LOA −0.28 g/dL (CI95% −0.53 to −0.02 g/dL). Conclusions For the first time, we provided data on SpHb accuracy and precision in the diabetic population. SpHb showed a high correlation coefficient when compared with Lab values, but the wide LOA limits its accuracy.

Growth performance, haematology and serum lipid profile of broiler chickens fed three varieties of ripe Solanum melongena fruit meal supplements

The growth, haematological and serum characteristics of Cobb broilers (n = 112) fed inclusions of ripe Solanum melongena fruit meal (SMFM) varieties were incorporated in a 56-day trial. The completely randomized design was employed in assigning day-old chicks to four labeled groups (T1-T4) with four replications. Dietary treatment of SMFM varieties was according to study groups such that T1 had Abia var. Okpokwe, T2 contained Nsukka Ind × Jos, T3 enclosed Nsukka Ind × Nsukka Local, while T4 was control. Daily feed intake and weekly weights furnished the growth assay of the chicks, while their blood assessment was done at the termination of the study. Data analyzed showed T4 to have better (p < 0.05) final weights (2740.50 g), feed intake (131.39 g) and weight gain (48.13 g) than SMFM groups. Red blood cell count was highest (p < 0.05) in T2 and T3 (10.87 × 106 and 10.88 × 106 mm3, respectively) and lowly in T1 and T4 (10.04 × 106 mm3 each). Haemoglobin concentration was highest (p < 0.05) in T2 and T3 (8.73, 8.98 g dL–) and lowest in T4 (7.55 g dL–1). Treated birds had better (p < 0.05) serum cholesterol and lipoproteins than the control. Triglyceride of T4 (117.75 g dL–1) was different (p < 0.05) from T1 and T2 (107.00, 107.67 g dL–1) but similar (p > 0.05) to T3 (109.00 g dL–1). The SMFM varieties, mostly Nsukka Ind × Jos (T2), independently promoted superior haematology and serum lipid profile but poor growth of test broilers.

Anaemia among Pregnant Women Attending Antenatal Care in Kyenjojo and Kole Districts in Uganda: Prevalence and Associated Factors

Anemia during pregnancy is a major public health problem, with a global prevalence of 41.8%. It is associated with high morbidity and mortality. Information on the prevalence of anemia and associated factors among pregnant women in the study area is limited. This study aimed at determining the prevalence and predictors of anemia among pregnant women attending antenatal clinics (ANC) in Kyenjojo and Kole Districts, Uganda. Cross-sectional study was conducted among randomly selected pregnant women. Quantitative data was collected using a structured questionnaire that captured demographic and obstetric characteristics. Haemoglobin concentration (Hb) was determined using HemoCue 201+. The study outcome was the prevalence of anaemia (Hb <11 g/dl). Chi-square, odds ratio, and logistic regression were used to test for associations. Of the total 760 pregnant women who participated in this study, 20.1% were anaemic. The prevalence varied from 13.4% in Kyenjojo to 26.9% in Kole District. Of 153 anaemic women, 121 (97%) were mildly anaemic, 27 (18%) moderately anemic, and 5(3%) severely anemic. Malaria infection [AOR: 0.46, 95% CI (0.26 – 0.83)], primigravida [AOR: 0.52, 95% CI: 0.29-0.93], and residing in Kole [AOR: 0.50, 95% CI: 0.32-0.76] were significant predictors of low haemoglobin concentration. This study highlighted the high prevalence of anaemia in our settings and the significant association between anaemia and malaria among pregnant women. Therefore, routine screening of pregnant women for anemia, malaria, and other risk factors during their first ANC visit is recommended to identify those at risk and prompt management provided to curb their negative consequences.

Heamatological parameters of one hump camels (camelusdromedarius) in North Western Nigeria

Camels are regarded as the ship of the desert, being used for transporting humans and their goods. They are also raised for milk, meat, hides and wool. Normal haematological values are important as disease diagnostic aid as they are vital indicator of the animals’ health status. Haematological parameters of one- humped camels (Camelusdromedarius) were determined in this study. 60 one- humped camels (Camelusdromedarius) were selected in sokoto and their blood samples were analyze using standard procedures for haematological parameters. [Packed cell volume (PCV), red blood cells (RBC), haemoglobin (Hb),white blood cells (WBC), neutrophils, eosinophils, Basophils, monocyte, lymphocyte, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)].For all the parameters, only neutrophils and MCV values showed significant variation between sexes with male camels having higher values than females. There is variation in the range values for all the parameters between sexes except Basophils, Monocyte and Lymphocyte. Based on the results of this study, the values were similar in both sex except in neutrophils and mean corpuscular volume indicating insignificant effect of sex on most of the haematological parameters.

Evaluation of Liver Function and Haemorheological Parameters in Some Carpenters Exposed to Wood Dust in Port Harcourt

Aim: To evaluate liver function and haemorheological parameters in some carpenters exposed to wood dust in Port Harcourt. Methodology: This cross-sectional study was carried out in some parts of Port Harcourt, the capital city of Rivers State in the southern part of Nigeria. The locations of the research included Sandfield mile 2 Diobu, Marine base, and Abuja by-pass Nkpolu-oroworukwo, Port Harcourt. Ethical approval and informed consent of the participants were obtained before commencement of the study. A total of one hundred and fifty (150) apparently healthy subjects aged between 20 t0 50 years were used for the study. Ninety (90) carpenters exposed to wood dust served as test subjects, while sixty (60) non- carpenters, non-wood workers not exposed to wood dust served as controls. Interviewer based sample questionnaire was administered to the subjects. Liver function parameters, Packed cell volume (PCV), erythrocyte sedimentation rate (ESR), haemoglobin concentration (Hb), and fibrinogen were analysed using standard laboratory techniques. Results: The mean age of the test subjects (36.5 ± 9.9 years) was not significantly different (P > .05) from that of the controls (32.1 ± 5.8 years). There were no significant differences (P > .05) in alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), Total protein (TP), albumin (ALB), total bilirubin (TB), conjugated bilirubin (CB), PCV and Hb in the test subjects compared to the controls. ESR (19.60 ± 10.67mm/Hr) and fibrinogen (624 ± 60.88ng/mL) were significantly higher (P< .05) in the tests (carpenters) compared to ESR (8.41 ± 5.36mm/Hr) and fibrinogen (448.4 ± 67.91ng/mL) in the controls. Conclusion: Wood dust exposure did not alter the liver function, PCV, and Hb levels of carpenters. However, with increases in ESR and fibrinogen in the carpenters, there may be a risk of inflammation to wood dust exposure in the carpenters, with negative consequences to the vasculature.

Chorioptes texanus causing mange in goats: comparison of two therapeutic protocols

Out of 34 caprine dermatological cases examined, 12 were detected positive for Chorioptes texanus. Pruritus, alopecia, crusts, thickening, wrinkling, cracks and fissures on legs, axillae, inguinal region and perineal region were the symptoms noticed in caprine chorioptic mange. In affected animals, reduction in haemoglobin concentration and per cent lymphocytes were noticed, along with elevation in the values of the total leukocyte count, per cent neutrophils and per cent monocytes. Values of serum glucose, zinc and copper were normal. All the 12 cases were treated with ivermectin at 10 days interval till two consecutive skin scrapings were negative for mites. In addition, all goats were treated with vitamin A supplements throughout the period. Six goats were treated topically with permethrin spray while other six animals were treated topically with lime sulphur spray. Both treatment protocols caused recovery of the animals from clinical symptoms, however, a skin scraping after 1 year revealed the presence of mites.

INDUCTION OF MACROCYTIC-NORMOCHROMIC ANAEMIA IN Clarias gariepinus –Siluriform- EXPOSED TO SUB-LETHAL CONCENTRATIONS OF 1, 1-DIMETHYL 4, 4-BIPIRIDILLIUM DICHLORIDE (PARAQUAT) UNDER LABPRATORY CONDITIONS

Introduction: Macrocytic-normochromic anaemia was induced in Clarias gariepinus at intervals of 1, 7 and 14 days. Experimental fish were exposed to test water separately diluted with sub-lethal concentrations of paraquat of 0, 0.03, 0.05, 0.07 and 0.09mg/L. 14 days. No in-depth work has been carried out on Macrocytic-normochromic anaemia in Nigeria. Objective: to evaluate macrocytic-normochromic anaemia in Clarias gariepinus exposed to sublethal concentrations of paraquat. Methods: Blood were collected at intervals of 1, 7 and 14 days .Results: Blood dyscrasias was observed with a significant (p<0.05) decrease in haemoglobin, haematocrit, red blood cells, white blood cells, lymphocytes and monocytes. Mean corpuscular volume (MCV), Mean corpuscular haemoglobin (MCH), Neutrophils, Eosinophil and Basophil increased significantly (p<0.05) with increasing concentrations of the toxicant while Mean corpuscular haemoglobin concentration (MCHC) remained normal. Conclusion: Sublethal concentrations of paraquat induced macrocytic-normochromic anaemia in the exposed Clarias gariepinus.

Progressive evaluation in spectroscopic sensors for non-invasive blood haemoglobin analysis - a review

Frequent monitoring of haemoglobin concentration is highly recommended by physicians to diagnose anaemia and polycythemia Vera. Moreover, Some other conditions also demand assessment of haemoglobin, and these conditions are blood loss, before blood donation, during pregnancy, preoperative, perioperative and postoperative conditions. Cyanmethaemoglobin/haemiglobincyanide method, portable haemoglobinometers and haematology analyzers are few standard methods to diagnose mentioned ailments. However, discomfort, delay and risk of infection are typical limitations of traditional measuring solutions. These limitations create the necessity to develop a non-invasive haemoglobin monitoring technique for a better lifestyle. Various methods and products are already developed and popular due to their non-invasiveness; however, invasive solutions are still considered as the reference standard method. Therefore, this review summarizes the attributes of existing non-invasive solutions. These attributes are finalized as brief details, accuracy, optimal benefits, and research challenges for exploring potential gaps, advancements and possibilities to consider as futuristic alternative methodologies. Non-invasive total haemoglobin assessing techniques are mainly based on optical spectroscopy (reflectance/transmittance) or digital photography or spectroscopic imaging in spot check/continuous monitoring mode. In all these techniques, we have noticed that there is a need to consider different light conditions, motion artefacts, melanocytes, other blood constituents, smoking and precise fixing of the sensor from the sensing spot for exact formulation. Moreover, based on careful and critical analysis of outcomes, none of these techniques or products is used independently or intended to replace invasive laboratory testing. Therefore there is a requirement for a more accurate technique that can eliminate the requirement of blood samples and likely end up as a reference standard method.

Comparative Efficacy of Two Antibiotics for the Management of Secondary Bacterial Infection in Goats Clinically Affected by Peste des Petits Ruminants

Aims: Peste des petits Ruminants (PPR) is a major viral disease that poses a challenge to small ruminant farming. Its natural occurrence has been complicated by secondary bacterial infection which has led to an increase in morbidity and mortality rates. This study reports the management outcome of natural PPR-infected goats using two types of antibiotics in Nsukka metropolis of Enugu State Nigeria. Methodology: Goats (N=24) were confirmed to be suffering from PPR based on clinical signs and using polymerase chain reaction (PCR). The animals were divided into two groups. Group A was treated with 20% oxytetracycline (N= 10) and group B with procaine penicillin and streptomycin combination (penstrept) (N= 14) injection. Clinical signs, recovery and survivability, temperature, haematology [Packed cell volume (PCV); haemoglobin concentration (Hbconc); red blood cell (RBC) and white blood cell (WBC) counts] and some serum biochemical profiles [alanine aminotransferase (ALT); aspartate transaminase (AST); total protein (TP); Albumin; urea; creatinine; potassium and sodium] were used to assess the efficacy of the antibiotics using standard techniques. Results: The mean temperature, RBC, WBC and urea values of the PPR-infected goats were above their reference ranges, mean albumin values were below the reference range while mean Hbconc, PCV, AST, ALT, TP and creatinine values were within their reference range before the commencement of treatment. Following treatment, clinical signs cleared in 20% and 35.7% of the goats treated with oxytetracycline and penstrep respectively. Death was recorded in 20% and 15% of goats treated with oxytetracycline and penstrep respectively before the end of treatment. Penstrep-treated group showed improvement in their haematological profile. Conclusion: Based on our findings, the use of penstrep in the management of PPR-infected goats gave a better result.

Blood Profile of Growing Rabbits Fed Pro-Vitamin A Fortified Cassava Peel Meal Based Diets

The effect of feeding pro-vitamin A cassava peel meal (PCPM) based diets to growing rabbits (n=48) for 61 days on their haematology and serum biochemical parameters was investigated. T1, T2, T3, and T4 diets were formulated with inclusion levels of 0, 15, 30, and 45%, respectively. In a completely randomized design, the rabbits were randomly allocated to four experimental groups of twelve animals each, with four rabbits constituting a replicate. On the last day of the study, blood samples were taken from each animal and analyzed for haematological and serum biochemical indices. The results of the proximate composition of experimental diets revealed that T2, T3, and T4 had high (p<0.05) ash values. T1 diet had higher (p<0.05) metabolizable energy (ME) in comparison with T3, and T4 diets. Red blood cells (RBC) and white blood cells (WBC) were improved (p<0.05) significantly in 15, 30, and 45% PCPM inclusions. T3 and T4 had better (p<0.05) packed cell volume (PCV) values, haemoglobin (Hb), mean cell haemoglobin (MCH), and mean cell haemoglobin concentration (MCHC) than T1 and T2. The treatment groups (T2, T3 and T4) had significantly higher (p<0.05) total protein, globulin, creatinine, total bilirubin, and aspartate amino transferase (AST). Cholesterol and urea levels were lowered significantly (p<0.05) in T3 and T4. All of the blood parameters were within the normal physiological range for clinically healthy rabbits, indicating that PCPM was beneficial to the rabbits' blood formation and health. T4 group had the best results and was recommended for enhanced rabbit production.