Superparamagnetic iron oxide labeling of spinal cord neural stem cells genetically modified by nerve growth factor-β

2009 ◽  
Vol 29 (2) ◽  
pp. 235-238 ◽  
Author(s):  
Deqiang Lei ◽  
Hongyang Zhao ◽  
Xingli Deng ◽  
Ruen Liu ◽  
Fangcheng Zhang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Iron Oxide ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Genetically Modified ◽  
Superparamagnetic Iron Oxide ◽  
Nerve Growth

scholarly journals Nerve growth factor (NGF) with hypoxia response elements loaded by adeno-associated virus (AAV) combined with neural stem cells improve the spinal cord injury recovery

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Qiuji Wu ◽  
Ziyue Xiang ◽  
Yibo Ying ◽  
Zhiyang Huang ◽  
Yurong Tu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Histochemical Staining ◽  
Adeno Associated Virus ◽  
Nerve Growth ◽  
Cord Injury

AbstractThe ischemia and hypoxia microenvironment after spinal cord injury (SCI) makes SCI repair a challenging problem. With various stimulus, chances for neural stem cells (NSCs) to differentiate into neurons, astrocytes, oligodendrocytes are great and is considered as a potential source of the stem cell therapy to SCI. Our research used adeno-associated virus (AAV) to carry the target gene to transfect neural stem cells. Transfected NSCs can express nerve growth factor (NGF) navigated by five hypoxia-responsive elements (5HRE). Therefore, the 5HRE-NGF-NSCs could express NGF specifically in hypoxia sites to promote the tissue repair and function recovery. Based on the regeneration of neurocytes and promotion of the recovery found in SCI models, via locomotor assessment, histochemical staining and molecular examinations, our results demonstrated that 5HRE-NGF-NSCs could improve the motor function, neurons survival and molecules expression of SCI rats. Meanwhile, the downregulated expression of autophagy-related proteins indicated the inhibitive effect of 5HRE-NGF-NSCs on autophagy. Our research showed that 5HRE-NGF-NSCs contribute to SCI repair which might via inhibiting autophagy and improving the survival rate of neuronal cells. The new therapy also hampered the hyperplasia of neural glial scars and induced axon regeneration. These positive functions of 5HRE-NGF-NSCs all indicate a promising SCI treatment.

scholarly journals Nerve Growth Factor, Neural Stem Cells and Alzheimer’s Disease

Neurosignals ◽  
2006 ◽  
Vol 15 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Klaus Heese ◽  
Jin Wei Low ◽  
Noriko Inoue
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Stem Cells ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Nerve Growth

Fibroblasts Genetically Modified to Produce Nerve Growth Factor Induce Robust Neuritic Ingrowth after Grafting to the Spinal Cord

1994 ◽  
Vol 126 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Mark H. Tuszynski ◽  
Daniel A. Peterson ◽  
Jasodhara Ray ◽  
Andrew Baird ◽  
Yasushi Nakahara ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Genetically Modified ◽  
Nerve Growth
Sign in / Sign up

Export Citation Format

Share Document