Osmotic stress induces terminal differentiation in cultured normal human epidermal keratinocytes

2008 ◽  
Vol 44 (5-6) ◽  
pp. 135-139 ◽  
Author(s):  
Thomas Mammone ◽  
Michael Ingrassia ◽  
Earl Goyarts
Keyword(s):  
Osmotic Stress ◽  
Terminal Differentiation ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Normal Human Epidermal Keratinocytes ◽  
Normal Human

scholarly journals MAPK pathway involved in epidermal terminal differentiation of normal human epidermal keratinocytes

Open Medicine ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. 189-195 ◽  
Author(s):  
Xianguang Meng ◽  
Liyun Qiu ◽  
Haiyan Song ◽  
Ningning Dang
Keyword(s):  
Signaling Pathway ◽  
Mapk Pathway ◽  
Terminal Differentiation ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Mapk Pathways ◽  
Normal Human Epidermal Keratinocytes ◽  
Normal Human ◽  
Related Proteins ◽  
Cytokeratin 14

AbstractObjectiveTo investigate the effect of mitogen-activated protein kinase (MAPK) signaling pathway in epidermal terminal differentiation.MethodsThe MAPK pathways (p38, ERK1/2, JNK) were inhibited by SB203580, PD98059, and SP600125 in normal human epidermal keratinocytes (NHEKs), respectively. Western blotting assays were performed to detect expression of filaggrin and differentiation-related proteins. The mRNA expressions of differentiation-related proteins were detected by real-time quantitative PCR (qRT-PCR).ResultsInhibition of MAPK pathway by SB203580, PD98059, and SP600125 resulted in significant reduction of filaggrin expression in NHEKs. Inhibition of the p38 MAPK pathway decreased the expression of differentiation-related proteins (cytokeratin 5, cytokeratin 14, ST14, and SPRR3), Akt, and NF-κB. Inhibition of JNK also suppressed expression of cytokeratin 14, SPRR3, Akt, and NF-κB. However, inhibition of ERK1/2 merely decreased expression of SPRR3 and Akt.ConclusionMAPK pathways regulates epidermal terminal differentiation in NHEKs. The p38 signaling pathway plays an especially important role.

scholarly journals Knock-down of filaggrin influences the mitogen-activated protein kinases signaling pathway in normal human epidermal keratinocytes

2018 ◽  
Vol 34 ◽  
pp. 94-98 ◽  
Author(s):  
Shumei Wang ◽  
Liyun Qiu ◽  
Xianguang Meng ◽  
Ningning Dang
Keyword(s):  
Signaling Pathway ◽  
Terminal Differentiation ◽  
Mapk Signaling ◽  
Epidermal Keratinocytes ◽  
Structural Protein ◽  
Human Epidermal Keratinocytes ◽  
Knock Down ◽  
Normal Human Epidermal Keratinocytes ◽  
Normal Human ◽  
Related Proteins

Background: Filaggrin is an essential structural protein of the stratum corneum binding to the keratin intermediate filaments to form a dense protein-lipid matrix. However, the function of filaggrin in epidermal terminal differentiation is not completely understood. Aim: To evaluate the effects of filaggrin on normal human epidermal keratinocytes (NHEKs) and to investigate the relevant mechanisms. Methods: Short hairpin RNA (shRNA) technology was used to knock-down filaggrin in normal human epidermal keratinocytes (NHEKs). Western blot and real-time quantitative PCR (qRT-PCR) were performed to detect expression of filaggrin, differentiation-related proteins and MAPK-related proteins. Results: Filaggrin was successfully knocked down in NHEKs (99% efficiency). We found that the lack of filaggrin significantly decreased the expression of some differentiation-related proteins, including Cytokeratin 5 protein, Cytokeratin 14 protein, ST14 protein and SPRR3 protein (P<0.05). In addition, filaggrin knock-down significantly decreased expression of p-p38, p-ERK1/2, p-JNK, p-Akt, and p-NF-κB in NHEKs. Conclusion: Our study shows that filaggrin regulates epidermal terminal differentiation and impairs MAPK signaling pathway in normal human epidermal keratinocytes.

Retinoic Acid Potentiates TNF-α-Induced ICAM-1 Expression in Normal Human Epidermal Keratinocytes

1999 ◽  
Vol 255 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Sophie Janssens ◽  
Luc Bols ◽  
Marc Vandermeeren ◽  
Guy Daneels ◽  
Marcel Borgers ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Normal Human Epidermal Keratinocytes ◽  
Tnf Α ◽  
Normal Human
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