Cell culture of the rice brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae)

2014 ◽  
Vol 50 (5) ◽  
pp. 384-388 ◽  
Author(s):  
Yipeng Xu ◽  
Yolanda H. Chen ◽  
Xiaoping Yu
Keyword(s):  
Cell Culture ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Nilaparvata Lugens Stål

scholarly journals Heat Stress Impedes Development and Lowers Fecundity of the Brown Planthopper Nilaparvata lugens (Stål)

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47413 ◽  
Author(s):  
Jiranan Piyaphongkul ◽  
Jeremy Pritchard ◽  
Jeff Bale
Keyword(s):  
Heat Stress ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Nilaparvata Lugens Stål

RNA interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

2015 ◽  
Vol 105 (4) ◽  
pp. 390-398 ◽  
Author(s):  
P.-J. Wan ◽  
L. Yang ◽  
S.-Y. Yuan ◽  
Y.-H. Tang ◽  
Q. Fu ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Transcript Level ◽  
Nilaparvata Lugens ◽  
The Body ◽  
Double Stranded Rna ◽  
Saccharopine Dehydrogenase ◽  
Body Weights ◽  
Nilaparvata Lugens Stål ◽  
Phloem Feeding ◽  
Wing Deformation

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.

scholarly journals Altered acetylcholinesterase as a resistance mechanism in the brown planthopper(Homoptera: Delphacidae), Nilaparvata lugens Stal.

2002 ◽  
Vol 37 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Jai-Ki Yoo ◽  
Si-Woo Lee ◽  
Yong-Joon Ahn ◽  
Toru Nagata ◽  
Toshio Shono
Keyword(s):  
Brown Planthopper ◽  
Resistance Mechanism ◽  
Nilaparvata Lugens ◽  
Nilaparvata Lugens Stål

scholarly journals Fluktuasi Populasi Serangga Wereng Batang Coklat (Nilaparvata lugens) Pada Lahan Sawah Di Kabupaten Kerawang Jawa Barat

Agrologia ◽  
2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Martua Suhunan Sianipar
Keyword(s):  
Regression Analysis ◽  
Natural Enemy ◽  
Diversity Index ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Population Fluctuation ◽  
Plant Pests ◽  
Nilaparvata Lugens Stål ◽  
Experimental Plots

Brown Planthopper (Nilaparvata lugens Stal.) is a main pest of paddy. The purpose of this research is to study the population of Brown Planthopper and the natural enemy. The method of this research using survey. Survey was conducted in three experimental plots at JatisariSubdistrict, Karawang District, West Javaand in the greenhouse of the Department of Plant Pests and Deseases, Faculty of Agriculture,  Universitas Padjadjaran. The sample were collected by systematically diagonal using yellow stiky trap and manually or visual counting. The result showed population fluctuation BPH below the economy line where is temperature, humidity and rainfall did not significantly effect to the BPH population. It was indicated by the regression analysis of each temperature Y= (- 24.225 + 1.008 X1 ; R2 =0,020; P/Sign = 0.660),  Humidity  (Y=- 32.337 + 0.424 X2; R2  = 0,124; P/ Sign  = 0.261) and Rainfall (Y=1.707 + 0.090 X3 ; R2  = 0.050; P/ Sign = 0.487). There is a relation between temperature, humidity and rainfall to BPH (Y = - 123 + 2.685 T + 0.649 RH - 0.14 CH; R2 = 0.236; P/ Sign = 0.516) dit not significantly because as the value of significance (P = Sign)  > 0.05.8. The diversity index was moderate 1.88

Sign in / Sign up

Export Citation Format

Share Document