scholarly journals Effects of plant growth regulators, carbon sources and pH values on callus induction in Aquilaria malaccensis leaf explants and characteristics of the resultant calli

2014 ◽  
Vol 25 (3) ◽  
pp. 535-540 ◽  
Author(s):  
Shashita Jayaraman ◽  
Nurul Hazwani Daud ◽  
Rasmina Halis ◽  
Rozi Mohamed
Author(s):  
Dwi Kusuma Wahyuni ◽  
Putri Andriani ◽  
Arif Nur Muhammad Ansori ◽  
Edy Setiti Wida Utami

<p class="IsiAbstrakIndo"><em>Justicia gendarussa </em>Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applying callus culture method. Accordingly, it is important to study the effects of plant growth regulators (2,4-D, IBA, and BAP) on callus induction of gendarussa leaves. This research design utilized a factorial design with two factors (2,4-D and IBA: 0.5, 1, 1.5 mg/L and BAP: 0.5, 1, 1.5, 2 mg/L). The experiment consisted of 24 treatments, each of which was repeated 3 times. Observation was carried out in 6 weeks. Data on the time of callus formation, percentage of explants formed callus, and callus morphology were analyzed descriptively,while data on fresh and dry weight were analyzed by Two-Way ANOVA (<span>α</span> = 0.5). Interestingly, the results showed that various concentration of plant growth regulators (2,4-D, IBA, and BAP) affected callus induction from leaf explants of gendarussa. We concluded that the most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2 mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable. <span lang="EN-GB">Moreover, t</span>he optimum treatment will be used to produce secondary metabolite and seed s<span lang="EN-GB">y</span>nthetic by cell suspension culture.</p>


2016 ◽  
Vol 2 (2) ◽  
pp. 320
Author(s):  
Brijit C. Dhas ◽  
Nimmi S. Simon ◽  
P. Austin ◽  
Sentamil Selvan ◽  
Shyam Kumar Rajaram ◽  
...  

2016 ◽  
Vol 26 (1) ◽  
pp. 85-96 ◽  
Author(s):  
Mohamed R Rady ◽  
El Sayed ◽  
M Mervat ◽  
Mohamed El Sayed ◽  
Usama I Aly

Leaf explants were cultured to evaluate the effect of different auxins and cytokinins and its concentrations; MS salts on micropropagation of the promising biodiesel Jatropha curcas plant under Egyptian conditions. Results showed that shoot initiated on 0.5 mg/l BA + 0.25 mg/l IBA. Multiplication and elongation were found to be the best using 0.5 mg/l BA in combination with 0.05 mg/l IBA. The multiple shoots were cultured on MS or half strength of MS supplemented with different concentrations of IAA and IBA for rooting phase. Half strength of MS containing 1.0 mg/l IAA was the best for rooting of micropropagated shoots. The rooted plantlets were acclimated in sand : peat?moss mixture (1 : 1) successfully.Plant Tissue Cult. & Biotech. 26(1): 85-96, 2016 (June)


Author(s):  
Nisha Malik ◽  
Rakesh Singh Sengar ◽  
Manoj Kumar Yadav ◽  
Shiv Kumar Singh ◽  
Gopal Singh ◽  
...  

2004 ◽  
Vol 7 (3) ◽  
pp. 331-334 ◽  
Author(s):  
Md. Monirul Islam ◽  
Sanjay Kumar Adhikar . ◽  
Purnendu Gain . ◽  
Md. Mizanur Rahman . ◽  
Noor-e-Alam Siddique .

1994 ◽  
Vol 58 (8) ◽  
pp. 1519-1521 ◽  
Author(s):  
Takanobu Takihara-Matsuura ◽  
Iwao Sakane ◽  
Takami Kakuda ◽  
Takuya Kitada ◽  
Tatsuyuki Kinoshita ◽  
...  

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