TaPT2, a high-affinity phosphate transporter gene in wheat (Triticum aestivum L.), is crucial in plant Pi uptake under phosphorus deprivation

2014 ◽  
Vol 36 (6) ◽  
pp. 1373-1384 ◽  
Author(s):  
Chengjin Guo ◽  
Li Guo ◽  
Xiaojuan Li ◽  
Juntao Gu ◽  
Miao Zhao ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Triticum Aestivum L ◽  
Phosphate Transporter ◽  
Transporter Gene ◽  
High Affinity ◽  
Phosphorus Deprivation

scholarly journals Identification of a High-Affinity Phosphate Transporter Gene in a Prasinophyte Alga, Tetraselmis chui, and Its Expression under Nutrient Limitation

2003 ◽  
Vol 69 (2) ◽  
pp. 754-759 ◽  
Author(s):  
Chih-Ching Chung ◽  
Sheng-Ping L. Hwang ◽  
Jeng Chang
Keyword(s):  
Amino Acid ◽  
Mrna Expression ◽  
Amino Acid Sequences ◽  
Phosphate Transporter ◽  
Molecular Probe ◽  
Transporter Gene ◽  
Reading Frame ◽  
Total Rna ◽  
High Affinity ◽  
Tetraselmis Chui

ABSTRACT A high-affinity phosphate transporter gene, TcPHO, was isolated from a growth-dependent subtracted cDNA library of the marine unicellular alga Tetraselmis chui. The full-length cDNA of TcPHO obtained by 5′ and 3′ rapid amplification of cDNA ends was 1,993 bp long and encoded an open reading frame consisting of 610 amino acids. The deduced amino acid sequence of TcPHO exhibited 51.6 and 49.8% similarity to the amino acid sequences of PHO89 from Saccharomyces cerevisiae and PHO4 from Neurospora crassa, respectively. In addition, hydrophobicity and secondary structure analyses revealed 12 conserved transmembrane domains that were the same as those found in PHO89 and PHO4. The expression of TcPHO mRNA was dependent on phosphate availability. With a low-phosphate treatment, the TcPHO mRNA concentration increased sharply to 2.72 fmol μg of total RNA−1 from day 1 to day 2 and remained at this high level from days 2 to 4. Furthermore, rescue treatment with either phosphate or p-nitrophenyl phosphate effectively inhibited TcPHO mRNA expression. In contrast, TcPHO mRNA expression stayed at a low level (range, 0.25 to 0.28 fmol μg of total RNA−1) under low-nitrate conditions. The expression pattern suggests that TcPHO can be used as a molecular probe for monitoring phosphorus stress in T. chui.

Sign in / Sign up

Export Citation Format

Share Document