Predicting Mycoplasma pneumoniae and Chlamydophila pneumoniae in community-acquired pneumonia (CAP) pneumonia: epidemiological study of respiratory tract infection using multiplex PCR assays

Acute respiratory tract infection (ARI) is a clinical condition which causes high morbidity and mortality, especially in infants and young children. Pneumonia is a common complication of respiratory tract infection. Atypical pneumonia, which is commonly caused by Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legi-onella pneumophila, is difficult to be detected because the causal bacteria cannot be diagnosed by routine culture method and presenting with non-specific clinical symptoms. This study aimed to diagnose the atypical pneumonia infection in children presenting with ARI attending Yangon Children’s Hospital during 2014-15 by using multiplex polymerase chain reaction (M-PCR). The bacterial DNA was extracted from nasopharyngeal swab samples by using Qiagen DNA minikit and detected by M-PCR. Of 245 patients with ARI, 140(57%) were males and 105(43%) were females. Eleven samples (4.4%) were positive for atypical pneumonia infection, among which 4(1.6%) were Mycoplasma pneumoniae,5(2%) were Chlamydophila pneumoniae and 2(0.8%) were Legionella pneumophila. The atypical pneumonia cases were mostly seen among the age of 1 to 5 years and sex distribution was nearly equal. The infected cases were detected from pneumonia (36.4%), severe pneumonia (27.3%), viral-induced wheeze (18.2%), severe bronchiolitis (9.1%) and bronchiolitis (9.1%). This study highlights the role of atypical pneumonia infection in ARI cases among children.

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Mycoplasma pneumoniae Respiratory Tract Infection Prevailing among Infants at a Nursery School

Kansenshogaku zasshi ◽

10.11150/kansenshogakuzasshi1970.66.1566 ◽

1992 ◽

Vol 66 (11) ◽

pp. 1566-1571

Author(s):

Kazuo MIMURA ◽

Kumiko SUGITA ◽

Kazuo TABUKI ◽

Tadafumi NISHIMURA

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Mycoplasma Pneumoniae ◽

Nursery School

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P1186 Implementation of national guidelines for good antibiotic policy of respiratory tract infection in hospitalised children with community-acquired pneumonia

International Journal of Antimicrobial Agents ◽

10.1016/s0924-8579(07)71026-9 ◽

2007 ◽

Vol 29 ◽

pp. S324

Author(s):

V. Maresova ◽

Z. Blechova ◽

M. Podojilová ◽

B. Horová ◽

K. Soltysova

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Community Acquired Pneumonia ◽

National Guidelines ◽

Antibiotic Policy

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Multiplex PCR in Respiratory Tract Infection diagnosis

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v9i1.31313 ◽

2017 ◽

Vol 9 (1) ◽

pp. 1-2

Author(s):

Ahmed Abu Saleh

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Multiplex Pcr ◽

Infection Diagnosis

Bangladesh J Med Microbiol 2015; 09 (01): 01-02

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Clinical Value of Two Detection Methods for Mycoplasma Pneumoniae Antibody in the Diagnosis of Mycoplasma Pneumoniae Infection in North China

10.21203/rs.3.rs-934275/v1 ◽

2021 ◽

Author(s):

Jupeng Wang ◽

Lina Zhu ◽

Mingkun Ma ◽

Hui Chen ◽

Suxiang Guo ◽

...

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Mycoplasma Pneumoniae ◽

North China ◽

Detection Methods ◽

Antibody Detection ◽

Positive Rate ◽

Adults And Children ◽

Males And Females

Abstract Background: To analyze the detection of Mycoplasma pneumoniae in adults and children in North China using two antibody detection methods, and to guide prevention and treatment.Methods: A retrospective study was conducted from September 2017 to May 2021. Mycoplasma pneumoniae total antibody was detected using particle agglutination (PA). Anti-Mycoplasma pneumoniae IgM in patients with respiratory tract infection was detected by indirect immunofluorescence. All patients were divided into 9 groups according to age: ≤1, 2-3, 4-6, 7-14, 15-18, 19-39, 40-59, 60-79, ≥80,Results: The positive rate of Mycoplasma pneumoniae total antibody in 5,666 patients with community-acquired pneumonia was 40.13%. In adults and children, the positive rates were 19.92% and 77.3% (p<0.05), respectively. The positive rates in males and females was 37.89% and 42.40% (p<0.05), respectively. The positive rate for anti-Mycoplasma pneumoniae IgM in 5,746 patients with respiratory tract infection was 28.56%, and 10.37% and 36.82% in adults and children (p<0.05), respectively. In males and females, the positive rate for anti-Mycoplasma pneumoniae IgM was 24.56% and 33.38% (p<0.05). The highest positive rates for total antibody and anti-Mycoplasma pneumoniae IgM were recorded in autumn. Of the 1,975 patients tested for Mycoplasma pneumoniae antibody using both methods simultaneously, 26.71% were negative for total antibody and 8.63% had titers ranging between 1:40 and 1:80 when positive for IgM antibody. When negative for anti-Mycoplasma pneumoniae IgM, total antibody titer was ≥1:160 in 34.94% of the patients.Conclusion: Mycoplasma pneumoniae is the main cause of respiratory tract infection and its incidence is highest in autumn. Because Mycoplasma pneumoniae was more commonly detected in women and children, screening should be strengthened in these groups.

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