scholarly journals Clinical Value of Two Detection Methods for Mycoplasma Pneumoniae Antibody in the Diagnosis of Mycoplasma Pneumoniae Infection in North China

2021 ◽  
Author(s):  
Jupeng Wang ◽  
Lina Zhu ◽  
Mingkun Ma ◽  
Hui Chen ◽  
Suxiang Guo ◽  
...  
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Mycoplasma Pneumoniae ◽  
North China ◽  
Detection Methods ◽  
Antibody Detection ◽  
Positive Rate ◽  
Adults And Children ◽  
Males And Females

Abstract Background: To analyze the detection of Mycoplasma pneumoniae in adults and children in North China using two antibody detection methods, and to guide prevention and treatment.Methods: A retrospective study was conducted from September 2017 to May 2021. Mycoplasma pneumoniae total antibody was detected using particle agglutination (PA). Anti-Mycoplasma pneumoniae IgM in patients with respiratory tract infection was detected by indirect immunofluorescence. All patients were divided into 9 groups according to age: ≤1, 2-3, 4-6, 7-14, 15-18, 19-39, 40-59, 60-79, ≥80,Results: The positive rate of Mycoplasma pneumoniae total antibody in 5,666 patients with community-acquired pneumonia was 40.13%. In adults and children, the positive rates were 19.92% and 77.3% (p<0.05), respectively. The positive rates in males and females was 37.89% and 42.40% (p<0.05), respectively. The positive rate for anti-Mycoplasma pneumoniae IgM in 5,746 patients with respiratory tract infection was 28.56%, and 10.37% and 36.82% in adults and children (p<0.05), respectively. In males and females, the positive rate for anti-Mycoplasma pneumoniae IgM was 24.56% and 33.38% (p<0.05). The highest positive rates for total antibody and anti-Mycoplasma pneumoniae IgM were recorded in autumn. Of the 1,975 patients tested for Mycoplasma pneumoniae antibody using both methods simultaneously, 26.71% were negative for total antibody and 8.63% had titers ranging between 1:40 and 1:80 when positive for IgM antibody. When negative for anti-Mycoplasma pneumoniae IgM, total antibody titer was ≥1:160 in 34.94% of the patients.Conclusion: Mycoplasma pneumoniae is the main cause of respiratory tract infection and its incidence is highest in autumn. Because Mycoplasma pneumoniae was more commonly detected in women and children, screening should be strengthened in these groups.

scholarly journals Epidemiology and Molecular Identification of Mycoplasma pneumoniae associated respiratory tract, Zhejiang province, China, 2008-2017

2019 ◽  
Author(s):  
Qian Jiang ◽  
Zhao Zhao ◽  
Fangfang Yang ◽  
Yumei Ge
Keyword(s):  
Tract Infection ◽  
Early Diagnosis ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Mycoplasma Pneumoniae ◽  
Acute Respiratory Tract Infection ◽  
Zhejiang Province ◽  
Taqman Probe ◽  
Positive Rate ◽  
Tract Infections

Abstract Background Mycoplasma pneumoniae is a common cause of respiratory infections in humans. The aim of this study was to investigate the infection of Mycoplasma pneumoniae (MP) in patients with acute respiratory tract infections in Zhejiang Province from 2008 to 2017, and to provide evidence for the early diagnosis and prevention of MP pneumonia. Methods MP-DNA was detected in nasopharyngeal swabs of patients with acute respiratory tract infection by real-time fluorescent PCR (Taqman probe). Statistical analysis and epidemiological investigation were carried out on the test results. Results There were 10296 patients with acute respiratory tract infection in Zhejiang People's Hospital from 2008 to 2017, including 4387 females and 5909 males. A total of 1251 MP-DNA positive patients were detected, with a total positive rate of 12.2% (1251/10296). Among 1251 patients with M. pneumoniae infection, 571 were female positive, with an average positive rate of 13.0% (571/4387), 680 were male positive, with a positive rate of 11.5% (680/5909). From 2008 to 2017, the positive rates were 22.8% (33 cases), 20.9% (211 cases), 20.9% (350 cases), 5.5% (70 cases), 11.7% (136 cases), 15.2% (190 cases), 7.8% (94 cases), 5.9% (62 cases), 7.8% (56 cases) and 6.0% (49 cases), respectively. Of 1251 MP-DNA positive patients, 1243 (99.4%) were younger than 18 years old. Conclusions MP infection mainly occurs in the age below 18 years old, suggesting that early diagnosis and prevention of MP infection in adolescents should be emphasized.

scholarly journals Mycoplasma pneumoniae Respiratory Tract Infection Prevailing among Infants at a Nursery School

1992 ◽  
Vol 66 (11) ◽  
pp. 1566-1571
Author(s):  
Kazuo MIMURA ◽  
Kumiko SUGITA ◽  
Kazuo TABUKI ◽  
Tadafumi NISHIMURA
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Mycoplasma Pneumoniae ◽  
Nursery School

scholarly journals Lower Respiratory Tract Infection in a Renal Transplant Recipient: Do not Forget Metapneumovirus

2012 ◽  
Vol 2012 ◽  
pp. 1-3 ◽  
Author(s):  
N. Noel ◽  
B. Rammaert ◽  
J. Zuber ◽  
N. Sayre ◽  
M. F. Mamzer-Bruneel ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Organ Transplant ◽  
Detection Methods ◽  
Solid Organ ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Solid Organ Transplant Recipients

Human metapneumovirus (hMPV) is emerging as a cause of a severe respiratory tract infection in immunocompromised patients. hMPVpneumonia has only been seldom reported in nonpulmonary solid organ transplanted patients, such as renal transplant recipients. We report here a case of a 39-year-old patient presenting with fever, cough, and interstitial opacities on CT scan diagnosed as a nonsevere hMPVpneumonia 11 years after a renal transplantation. Infection resolved spontaneously. Differential diagnosis withPneumocystispneumonia was discussed. We review the medical literature and discuss clinical presentation and detection methods that can be proposed in solid organ transplant recipients.

scholarly journals The study of Mycoplasma pneumonia infection among children with respiratory tract infection in hospital in Chengdu from 2014 to 2020

2021 ◽  
Author(s):  
lei zhang ◽  
Meimei Lai ◽  
Tao Ai ◽  
Huilling Liao ◽  
Yijie Huang ◽  
...  
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Incidence Rate ◽  
Respiratory Diseases ◽  
Hospitalized Children ◽  
Temperature And Humidity ◽  
Mycoplasma Pneumonia ◽  
Positive Rate ◽  
Tract Infections

Abstract Aim: The hospitalized children with Mycoplasma pneumonia (M. pneumonia) infection caused by respiratory tract infection in Chengdu were studied and analysis of the epidemiological characteristics was carried out to provide a theoretical basis for clinical diagnosis and treatment.Method: 22882 hospitalized children with respiratory tract infections between January 2014 and December 2020 were collected M. pneumonia IgM antibody was detected by indirect immunofluorescence method and passive agglutination method. Demographic characteristics, clinical diagnose and laboratory data of these children were analyzed.retrospectively.. Result : The 4213 specimens with M. pneumonia were tested positive, the total positive rate was18.41%(18.30% in male and 22.72% in female). Higher positive rates were found in female children,Look from the statistical analysis results, the consistency between the two sets of data is low（ｘ2=198.078、P＜0.01）. The results of different age patients with contrast different M. pneumonia infection degree were statistically significant（F=162.7532、P＜0.01）,there was higher M. pneumonia positive rate in Preschoolers and school-age children ,33.98% and 32.98%, respectively.The incidence rate of M. pneumonia in 2017 and 2019 was significantly higher than average （F=538.95, P＜0.01)The difference of incidence rate of M. pneumonia was not significant in different months in 2014, 2015 and 2020 (P>0.05). But the probability of M .pneumonia infection patients was much higher from April to May and September to October in2016,2017,2018 and 2019（P＜0.05）. There was no correlation about M. pneumonia infection with temperature and humidity( P＞0.05)，there was negative correlation with PM2.5（R=0.09362, P<0.01）and PM10.（R=0.1185, P<0.01）.There was no difference about constituent ratio of case of M. pneumonia infection between 2014 and 2019 (F=32.34,P>0.05).The Common respiratory diseases of M. pneumonia infection, bronchopneumonia accounts for the highest proportion,followed the exacerbation of asthma and severe pneumonia.There was significantly difference about constituent ratio of case of M. pneumonia infection between in 2020 and in other years (F=159.35,P<0.01) .The Common respiratory diseases of M. pneumonia infection, bronchopneumonia accounts for the highest proportion,followed the acute bronchitis and exacerbation of asthma.Conclusion:The distribution and epidemiological trend of M. pneumonia in patients with respiratory tract infection showed the risk of inflammation was connected with the gender, age, year and month, no relationship with temperature and humidity in Chengdu,.Higher M. pneumonia positive rate was shown in the children with bronchial pneumonia and exacerbation of asthma.The prevention measures which controlled the COVID-19 disease had effectively controlled the infection rate of M. pneumonia.

Asthmatic Children Have Increased Specific Anti–Mycoplasma pneumoniae IgM but not IgG or IgE—Values Independent of History of Respiratory Tract Infection

2013 ◽  
Vol 32 (6) ◽  
pp. 599-603 ◽  
Author(s):  
Tamar A. Smith-Norowitz ◽  
Jonathan I. Silverberg ◽  
Melanie Kusonruksa ◽  
Diana Weaver ◽  
David Ginsburg ◽  
...  
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Mycoplasma Pneumoniae ◽  
Asthmatic Children ◽  
History Of

scholarly journals Development of loop-mediated isothermal amplification coupled with nanoparticle-based lateral flow biosensor assay for Mycoplasma pneumoniae detection

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yacui Wang ◽  
Yi Wang ◽  
Weiwei Jiao ◽  
Jieqiong Li ◽  
Shuting Quan ◽  
...  
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Real Time ◽  
Mycoplasma Pneumoniae ◽  
Real Time Pcr ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Dna Templates ◽  
Loop Mediated Isothermal Amplification

AbstractMycoplasma pneumoniae (MP) is one of the most common pathogens causing respiratory tract infection, especially for community-acquired pneumonia (CAP) in school-age children. There was considerable amount of studies on loop-mediated isothermal amplification (LAMP) assay for MP detection. However, the result interpretation of these developed LAMP assays was sophisticated and subjective. Therefore, we developed and evaluated a LAMP coupled with nanoparticle-based lateral flow biosensor (LFB) assay (LAMP-LFB) for simple, reliable, and objective identification of MP (MP-LAMP-LFB). Six primers specific to P1 gene of MP were designed, and the preferred temperature for this assay was confirmed to be 65 °C. The amplification products could be visually interpreted by LFB within 2 min. The MP-LAMP-LFB assay specifically identified DNA templates of MP, and no cross-reactivity with other pathogens was obtained. The limit of the detection for this assay was 600 fg of DNA templates in pure cultures, which was in complete accordance with colorimetric indicator detection and agarose gel electrophoresis analysis. This assay was applied to 209 oropharyngeal swab specimens collected from children with acute respiratory tract infection for clinical evaluation, and compared to real-time PCR detection. Using the LAMP-LFB and real-time PCR assay, the positive rates of MP were 47.8% and 31.6%, respectively. Results suggested that the LAMP-LFB assay displayed high sensitivity compared to real-time PCR method. In summary, LAMP-LFB assay established here was a simple, objective, and sensitive assay for MP detection, which can be widely applied in clinical settings, especially in rural areas.

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