Removal of N-terminal methionine of human interferon α-2b by co‐producing with Pyrococcus furiosus methionine aminopeptidase in Escherichia coli

Biologia ◽  
2021 ◽  
Author(s):  
Amina Arif ◽  
Naeem Rashid ◽  
Muhammad Akhtar
Keyword(s):  
Escherichia Coli ◽  
Pyrococcus Furiosus ◽  
Human Interferon ◽  
Interferon Α ◽  
Methionine Aminopeptidase ◽  
Human Interferon Α

scholarly journals Preventing the N-terminal processing of human interferon α-2b and its chimeric derivatives expressed in Escherichia coli

2018 ◽  
Vol 76 ◽  
pp. 294-302 ◽  
Author(s):  
Fatima Ahsan ◽  
Qurratulann Afza Gardner ◽  
Naeem Rashid ◽  
Greg J. Towers ◽  
Muhammad Akhtar
Keyword(s):  
Escherichia Coli ◽  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α

Isolation of a Biologically Active Soluble Human Interferon-α Receptor-GST Fusion Protein Expressed in Escherichia coli

1996 ◽  
Vol 16 (10) ◽  
pp. 835-844 ◽  
Author(s):  
NGA Y. NGUYEN ◽  
DAN SACKETT ◽  
ROSARIO D.C. HIRATA ◽  
DAVID E. LEVY ◽  
JOAN C. ENTERLINE ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Biologically Active ◽  
Human Interferon ◽  
Interferon Α ◽  
Gst Fusion Protein ◽  
Human Interferon Α

Production of human interferon alpha-2b in Escherichia coli and removal of N-terminal methionine utilizing archaeal methionine aminopeptidase

Biologia ◽  
2015 ◽  
Vol 70 (7) ◽  
Author(s):  
Amina Arif ◽  
Qura-Tul-Ann A. Gardner ◽  
Naeem Rashid ◽  
Muhammad Akhtar
Keyword(s):  
Escherichia Coli ◽  
Interferon Alpha ◽  
Inclusion Bodies ◽  
High Performance ◽  
Pyrococcus Furiosus ◽  
Human Interferon ◽  
Methionine Aminopeptidase ◽  
Interferon Alpha 2B ◽  
Foreign Proteins ◽  
Insoluble Form

AbstractMethionine is the first amino acid residue in most of the recombinant proteins and sometimes it is necessary to remove the starting methionine in order to get foreign proteins with wild type sequences. Recombinant human interferon alpha-2b was produced in Escherichia coli in insoluble form as inclusion bodies, which were solubilized and refolded. Analysis of the purified recombinant protein indicated that it consisted of two types of molecules, one type, in majority, having N-terminal methionine intact, whereas the other type, in minority, having the N-terminal methionine cleaved by methionine aminopeptidase of the host. Both species were separated by high performance liquid chromatography and Nterminal methionine of the remaining molecules was removed by utilizing methionine aminopeptidase from Pyrococcus furiosus in order to get similar form of the interferon alpha-2b as produced by human body. The methodology developed in this study can be extended to other proteins of pharmaceutical importance.

scholarly journals Generation of transgenic fibroblasts producing doxycycline-inducible human interferon-α or erythropoietin for a bovine mammary bioreactor

2012 ◽  
Vol 12 (1) ◽  
pp. 1137-1144 ◽  
Author(s):  
HEE YOUNG KANG ◽  
EUI-JU HONG ◽  
KYU-CHAN HWANG ◽  
NAM-HYUNG KIM ◽  
WOO-SUK HWANG ◽  
...  
Keyword(s):  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α

Long-term stabilization of recombinant human interferon α 2b in aqueous solution without serum albumin

2003 ◽  
Vol 264 (1-2) ◽  
pp. 57-72 ◽  
Author(s):  
Llamil Ruiz ◽  
Nuria Reyes ◽  
Ladys Duany ◽  
Abrisleyda Franco ◽  
Kethia Aroche ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Serum Albumin ◽  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α

Human interferon-α induces immobility in the mouse forced swimming test: involvement of the opioid system

2000 ◽  
Vol 852 (2) ◽  
pp. 482-484 ◽  
Author(s):  
Mitsuhiro Makino ◽  
Yutaka Kitano ◽  
Chika Komiyama ◽  
Masaaki Hirohashi ◽  
Morihiro Kohno ◽  
...  
Keyword(s):  
Forced Swimming Test ◽  
Forced Swimming ◽  
Opioid System ◽  
Human Interferon ◽  
Interferon Α ◽  
Swimming Test ◽  
Human Interferon Α

A genetic study of human interferon-α-induced repair of DNA damage in hepatitis B patients

1991 ◽  
Vol 262 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Peilin Wang ◽  
Shuying Cong ◽  
Xiaoyong Dong ◽  
Zaichun Chen ◽  
Chuiping Ma
Keyword(s):  
Dna Damage ◽  
Hepatitis B ◽  
Genetic Study ◽  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α
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