Identification of dominant microbial community in aerophilic biofilm reactors by fluorescence in situ hybridization and PCR-denaturing gradient gel electrophoresis

2009 ◽  
Vol 26 (3) ◽  
pp. 685-690 ◽  
Author(s):  
Youngo Kim ◽  
Taeho Lee ◽  
Taejoo Park ◽  
Heesu Park ◽  
Sanghyup Lee
Keyword(s):  
In Situ Hybridization ◽  
Microbial Community ◽  
Fluorescence In Situ Hybridization ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Biofilm Reactors ◽  
Gradient Gel Electrophoresis

Degradation of Nonylphenol Polyethoxylates and its Microflora Structure in an Anoxic-Oxic Activated Sludge Process

2012 ◽  
Vol 610-613 ◽  
pp. 331-336
Author(s):  
Yuan Hua Xie ◽  
Tong Zhu ◽  
Xiao Jiang Liu ◽  
Hui Liu ◽  
Jin Han
Keyword(s):  
In Situ Hybridization ◽  
Activated Sludge ◽  
Removal Efficiency ◽  
Gel Electrophoresis ◽  
Loading Rate ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Oxygen Demand ◽  
Activated Sludge Process ◽  
Gradient Gel Electrophoresis

An anoxic-oxic activated sludge process (AOASP) was carried out to degrade nonylphenol polyethoxylates (NPEOs). The carbon source in influent was replaced stepwise by a mixture of nonylphenol decaethoxylate (M-NP10EO). The 2nd-derivative UV-spectrometry was applied to determine the total amount of M-NP10EO in water samples. Chemical oxygen demand (COD) removal efficiency achieves about 85% under the highest M-NP10EO loading rate, and M-NP10EO removal efficiency is about 80%. Denaturing gradient gel electrophoresis (DGGE) results of activated sludges show that the microbe species decrease but gradually stabilize with the increase of M-NP10EO concentration in influent. Fluorescence in situ hybridization (FISH) results of activated sludges showe that the dominant microflora under the highest M-NP10EO loading rate is β-Proteobacteria (35%), followed by α-Proteobacteria (15%), γ-Proteobacteria (5%) and Actinobateria (4%).

Analysis of bacterial community in membrane-separation bioreactors by fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) techniques

2000 ◽  
Vol 41 (10-11) ◽  
pp. 259-268 ◽  
Author(s):  
B.S. Luxmy ◽  
F. Nakajima ◽  
Kazuo Yamamoto
Keyword(s):  
In Situ Hybridization ◽  
Bacterial Communities ◽  
Gel Electrophoresis ◽  
Fluorescent In Situ Hybridization ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Membrane Separation ◽  
Diversity Index ◽  
Ammonia Oxidizing Bacteria ◽  
Gradient Gel Electrophoresis

The bacterial communities of membrane-separation bioreactors (MBR) fed with raw sewage were analyzed by a pilot scale study. The community was analyzed by both Fluorescent in Situ Hybridization (FISH) and PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) techniques. Five rRNA targeted group specific oligonucleotide probes showed that the alpha- and beta- subclasses of proteobacteria were the most dominant groups among them. The identification of ammonia-oxidizing bacteria in MBR was confirmed by three probes: NEU, Nsv 443 and Nso 190. Mostly the ammonia-oxidizers were found in groups and present in the form of clusters or aggregates. The ratio of NEU/EUB was estimated by double hybridization and image analysis techniques as 6%. The Nitrobacter sp. was also identified inside the MBR with the help of a NIT3 probe and they were also found to be present in the form of a cluster. Usually the clusters formed by the Nitrobacter sp. were smaller than those of ammonia-oxidizing groups. After numerical analysis on the band pattern of DGGE, it was found that the MBR bacterial communities were different from that of conventional activated sludge (CAS) communities with dissimilarity indexes more than 0.6. The diversity of the microbial community was estimated by the Shannon-Weaver index of general diversity. It was found that the value of the diversity index for the CAS process was 1.61 while those for two MBR processes were 1.68 and 1.59.

Study of microbial community of brewery-treating granular sludge by denaturing gradient gel electrophoresis of 16S rRNA gene

2001 ◽  
Vol 43 (1) ◽  
pp. 77-82 ◽  
Author(s):  
O.-C. Chan ◽  
W.-T. Liu ◽  
H. H. Fang
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Rrna Gene ◽  
Related Sequence ◽  
Gradient Gel Electrophoresis

The microbial community structure of granular sludge from an upflow anaerobic sludge blanket (UASB) reactor treating brewery effluent was studied by denaturing gradient gel electrophoresis (DGGE). Twelve major bands were observed in the DGGE fingerprint for the Bacteria domain and four bands for the Archaea domain. Of the bacterial bands observed, six were successfully purified and sequenced. Among them, three were related to the gram-positive low G+C group, one to the Delta subclass of the Proteobacteria, one to the Gamma subclass, and one to the Cytophaga group with no close related sequence. The 16S rRNA sequences of the four archaeal bands were closely associated with Methanosaeta concilii and Methanobacterium formicum.

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