In vitro plantlet regeneration from nodal explants of field-grown culms in Bambusa glaucescens Willd.

2007 ◽  
Vol 1 (3) ◽  
pp. 141-147 ◽  
Author(s):  
Fatima Shirin ◽  
P. K. Rana
Keyword(s):  
Plantlet Regeneration ◽  
Nodal Explants

scholarly journals Efficient in vitro culture protocols for propagating Phalaenopsis ‘Cool Breeze’

2015 ◽  
Vol 24 (2) ◽  
pp. 191-203 ◽  
Author(s):  
Kh Balilashaki ◽  
R Naderi ◽  
S Kalantari ◽  
M Vahedi
Keyword(s):  
Plant Tissue ◽  
Large Scale ◽  
Economic Value ◽  
Shoot Formation ◽  
Plantlet Regeneration ◽  
Nodal Explants ◽  
Cut Flowers ◽  
Flower Stalk ◽  
Regenerated Plants

Phalaenopsis orchids have high economic value in the floriculture industry as cut flowers and potted plants throughout the world. Plant tissue culture technology is being widely used for large scale plant multiplication of Phalaenopsis to feed into this industry. In order to increase the efficiency of this technology, four experiments were undertaken: Plantlet regeneration from seeds, nodes or leaves, and hardening of the regenerated plants. In the first experiment, seed germination was examined in three media (half MS, Chen and Vacin?Went) of which the Chen medium had the best result (83.4%) in comparison to the other two media. In the second experiment, nodes on the flower stalk were studied for their shoot formation potential to different concentrations of BA and NAA, The highest frequency of shoot regeneration was achieved on MS containing 4 mg/l BA and 1 mg/l NAA while in vitro derived leaves formed clusters of somatic embryos directly when cultured on MS containing TDZ at different concentrations (0.5, 1, 2 and 3 mg/l). The embryos turned green and developed into protocorm?like bodies after 7 weeks of culture followed by plantlet regeneration. The highest plantlet regeneration from the leaf?derived embryos was obtained from MS supplemented with 3 mg/l TDZ. Finally, regenerated plants from (seeds, nodal explants and leaves) were compared in two medium for hardening, regenerated plant from nodal explants showed the highest survival rate (100%) on the medium containing cocopeat, coal, industrial cartridge and the bites of yonolit (1 : 1 : 2 : 4).Plant Tissue Cult. & Biotech. 24(2): 191-203, 2014 (December)

scholarly journals In vitro callus induction and plantlet regeneration of Indian red wood, Soymida febrifuga A. Juss (Roxb.)

2014 ◽  
Vol 8 (24) ◽  
pp. 847-856
Author(s):  
K. Chiruvella Kishore ◽  
Mohammed Arifullah ◽  
Gopal Ghanta Rama ◽  
K. Chiruvella Kishore ◽  
Mohammed Arifullah ◽  
...  
Keyword(s):  
Callus Induction ◽  
Plantlet Regeneration

scholarly journals An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

2009 ◽  
Vol 18 (1) ◽  
pp. 17-24
Author(s):  
Md. Nesawar Miah ◽  
Shahina Islam ◽  
Syed Hadiuzzaman
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Cow Dung ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

In vitro plantlet regeneration of Olea europaea ssp. maderensis

2003 ◽  
Vol 97 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Conceição V. Santos ◽  
Gina Brito ◽  
Gloria Pinto ◽  
Henrique M.A.C. Fonseca
Keyword(s):  
Olea Europaea ◽  
Plantlet Regeneration ◽  
Olea Europaéa

scholarly journals In vitro regeneration and induction of multiple shooting in Cicer arietinum L. using cotyledonary nodal explants

2015 ◽  
Vol 14 (13) ◽  
pp. 1129-1138 ◽  
Author(s):  
Prema Sunil Sruthi ◽  
Philip Robinson J ◽  
S KarthickBalan S ◽  
Anandhaprabhakaran M ◽  
Balakrishnan V
Keyword(s):  
Cicer Arietinum ◽  
In Vitro Regeneration ◽  
Nodal Explants ◽  
Multiple Shooting ◽  
Cicer Arietinum L

scholarly journals In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants

2011 ◽  
Vol 39 (1) ◽  
pp. 84 ◽  
Author(s):  
Kantamaht KANCHANAPOOM ◽  
Suttinee JINGJIT ◽  
Kamnoon KANCHANAPOOM
Keyword(s):  
Multiple Shoots ◽  
Shoot Formation ◽  
In Vitro Flowering ◽  
Nodal Explants ◽  
Callus Growth ◽  
Ms Medium ◽  
Old Field ◽  
Gypsophila Paniculata ◽  
Initial Culture

A protocol for the regeneration of Gypsophila paniculata L. using nodal explants from 2-month-old field grown plants was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 13.3 μM BA. Callus growth was observed on MS medium containing 44.3 μM BA. Calluses were transferred to MS medium supplemented with 2, 4-D (4.5, 13.5, 22.6 μM), NAA (5.3, 16.1, 26.8 μM) or BA (4.4, 13.3, 22.1 μM) for 2 months to induce shoot formation. After 6 weeks of initial culture, multiple shoots were regenerated from calluses cultured on MS medium supplemented with 13.3 μM BA. All regenerated shoots produced roots on 16.1 μM NAA containing MS medium within 4 weeks. Rooted plantlets were hardened and established in pots at 100% survival. For induction of in vitro flowering, regenerated shoots could be induced to flower efficiently when cultured on MS medium containing 13.3 μM BA and 50 g/l sucrose.

scholarly journals Inhibition of browning problem during the callogenesis of Spartium junceum L.

2021 ◽  
Vol 27 (1) ◽  
pp. 68-77
Author(s):  
Mina Taghizadeh ◽  
Mahboubeh Ganji Dastjerdi
Keyword(s):  
In Vitro Culture ◽  
Callus Formation ◽  
Nodal Explants ◽  
Antioxidant Compounds ◽  
Induction Phase ◽  
Natural Habitats ◽  
Running Water ◽  
Changing Light ◽  
Spartium Junceum

Abstract During different phases of in vitro culture, plant tissues may be exposed to some stresses that never encounter in their natural habitats. The most significant stresses which interfere with in vitro culture are pathogenic contamination and browning disorder. Since browning sign is occurred during all phases of in vitro culture of Spartium junceum L., the present study was done preventing explants from browning during disinfection and callogenesis phases using exposure time of sterilants (ethanol 0, 30, 60 s and home bleach 0, 10, 15 min), antioxidant compounds (PVP 0.5%, Activated charcoal 0.1%, Curcumin 0.1%), Running water (30 and 60 min) plant growth regulators (2,4-D 0, 0.5, 1 and 2 mg L-1 and BA 0, 0.1 and 0.2 mg L-1), and by changing light/dark conditions was designed. The results showed that ethanol 70% (30 s) in combination with home bleach 20% (10 min) had the best effect in control contaminations and browning sign in nodal explants of S. junceum. The application of PVP 0.5% in medium was the best treatment to control of browning nodal explants in callus induction phase. The highest callus formation and the lowest explant browning were obtained on the medium supplemented with 0.5 mg L-1 2,4-D under the darkness condition. According to the results of this study, how disinfection methods, culture medium compositions and light conditions were effective on the browning and callogenesis of Spartium junceum L.

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