A methodological study of leaf spring by material comparison and Taguchi’s DOE

Author(s):  
Sagarsingh Kushwah ◽  
Shreyashkumar Parekh ◽  
Harsh Mistry ◽  
Meet Bhatt ◽  
V. K. Joshi
2012 ◽  
Author(s):  
Katie M. Edwards ◽  
Kateryna M. Sylaska ◽  
Arianna K. Schaaff ◽  
Megan J. Murphy ◽  
Christine A. Gidycz

1972 ◽  
Vol 27 (03) ◽  
pp. 610-618 ◽  
Author(s):  
H Graeff ◽  
R von Hugo

SummaryThe observation of fibrinogen derivatives with a molecular weight higher than the parent molecule in human cases of DIC initiated the present methodological study. These derivatives were identified by the following methods : 2.5 M β-alanine precipitation of the plasma samples, PAA gel electrophoresis, intra gel immunoprecipitation and agarose gel chromatography. In the plasma of a patient with severe eclampsia and laboratory signs of DIC two derivatives with a molecular weight higher than that of fibrinogen were identified according to their relative electrophoretic mobilities: 0.18 and 0.28 × 10−5 cm2/V × sec (fibrinogen: 0.43 × 10−5 cm2/V × sec). Electrophoretic studies in the presence of 5 M urea indicated that the 0.28 derivative is a complex probably formed by fibrinogen and a fibrin monomer.


1972 ◽  
Vol 71 (1) ◽  
pp. 24-36 ◽  
Author(s):  
Ariel Gordin ◽  
Pirkko Saarinen

ABSTRACT An account is given of a methodological study of the double-antibody radioimmunoassay of human TSH, using highly purified labelled human TSH as tracer. It was shown that conventional paper electrophoresis was not adequate for studying the purity of labelled human TSH. When polyvinylchloride (Pevikon®) electrophoresis was used, four subfractions could still be separated, even though, on paper electrophoresis, the material seemed to be homogeneous. Only two of the four Pevikon fractions were immunoreactive. Purification of labelled human TSH by Pevikon electrophoresis also improved the sensitivity of the assay. Specific activities of about 100 mCi/mg gave the highest initial binding capacity, produced least damage to the labelled hormone and showed the best stability of the tracer without influencing the sensitivity of the method. In different storage conditions, labelled human TSH was found to be most stable at −20°C and diluted 1/100. Only in pregnancy did the addition of HCG seem necessary. The mean TSH value in healthy subjects was 3.6 ± 1.4 μU/ml (mean±sd) with a range from 1.6 μU/ml to 8.8 μU/ml.


2011 ◽  
pp. 45-64 ◽  
Author(s):  
Peter Razzell

A review of evidence on infant mortality derived from the London bills of mortality and parish registers indicates that there were major registration problems throughout the whole of the parish register period. One way of addressing these problems is to carry out reconstitution studies of individual London parishes, but there are a number of problems with reconstitution methodology, including the traffic in corpses between parishes both inside and outside of London and the negligence of clergymen in registering both baptisms and burials. In this paper the triangulation of sources has been employed to measure the adequacy of burial registration, including the comparison of data from bills of mortality, parish registers and probate returns, as well as the use of the same-name technique. This research indicates that between 20 and 40 per cent of burials went unregistered in London during the parish register period.


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