Statistical Determination of Optimal Baculovirus Infection Condition for Recombinant Protein Production in Drosophila S2 Cells

2007 ◽  
Vol 142 (3) ◽  
pp. 243-252
Author(s):  
Hye Sook Cho ◽  
Kyoung Ro Kim ◽  
Yeon Kyu Kim ◽  
Hyung Joon Cha
Keyword(s):  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
S2 Cells ◽  
Baculovirus Infection ◽  
Drosophila S2 Cells ◽  
Statistical Determination

Overexpression of c-Myc enhances recombinant protein production in High Five cells after baculovirus infection

2018 ◽  
Vol 73 (3-4) ◽  
pp. 147-151
Author(s):  
Xiaoli Ju ◽  
Meijia Ren ◽  
Keping Chen ◽  
Qiang Wang
Keyword(s):  
Recombinant Protein ◽  
Cell Lines ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Control Cell ◽  
Stable Cell Lines ◽  
Vector Systems ◽  
Baculovirus Infection ◽  
Baculovirus Expression Vector ◽  
High Five Cells

AbstractDue to their numerous advantages, baculovirus expression vector systems (BEVS) have been widely used to express recombinant proteins for different purposes. Different strategies have been adopted to increase recombinant protein production. In this study, we transiently or stably expressed mousec-Mycin High Five cells using a commercial pIB/V5 vector. Under the control of theOpIE2promoter, this vector could enhance recombinant protein production. We found that transient expression ofc-Mycin High Five cells improved recombinant protein production. Furthermore, we established two stable cell lines, High Five-c-Myc #1 and High Five-c-Myc #2, that stably expressed mousec-Myc. We further found that the expression level of the recombinant protein was increased in these stable cell lines compared to control cell lines. These data indicate that overexpressingc-Mycin cells is a promising way to improve recombinant protein production in BEVS.

Continuous bleed recycling significantly increases recombinant protein production yield in perfusion cell cultures

2021 ◽  
Vol 169 ◽  
pp. 107966
Author(s):  
Jean-Marc Bielser ◽  
Mathieu Aeby ◽  
Stefania Caso ◽  
Anaïs Roulet ◽  
Hervé Broly ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Cell Cultures ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Production Yield
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