Characterization of Unique and Differentially Expressed Proteins in Anthracnose-Tolerant Florida Hybrid Bunch Grapes

2008 ◽  
Vol 157 (3) ◽  
pp. 395-406 ◽  
Author(s):  
Hemanth K. N. Vasanthaiah ◽  
Ramesh Katam ◽  
Sheikh M. Basha
Keyword(s):  
Differentially Expressed ◽  
Differentially Expressed Proteins

Proteomic characterization of differentially expressed proteins in breast cancer: Expression of hnRNP H1, RKIP and GRP78 is strongly associated with HER-2/neu status

2008 ◽  
Vol 2 (1) ◽  
pp. 99-107 ◽  
Author(s):  
Daohai Zhang ◽  
Lee Kian Tai ◽  
Lee Lee Wong ◽  
Thomas C. Putti ◽  
Sunil K. Sethi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Her 2

scholarly journals Proteomic Characterization of Yersinia pestis Virulence

2005 ◽  
Vol 187 (23) ◽  
pp. 8172-8180 ◽  
Author(s):  
Brett A. Chromy ◽  
Megan W. Choi ◽  
Gloria A. Murphy ◽  
Arlene D. Gonzales ◽  
Chris H. Corzett ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Differentially Expressed ◽  
Virulence Determinants ◽  
Differentially Expressed Proteins ◽  
Mechanistic Studies ◽  
Differentially Expressed Protein ◽  
Catalase Peroxidase ◽  
Membrane Bound ◽  
Differential Gel Electrophoresis

ABSTRACT The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Caf1), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.

Identification, N-terminal region sequencing and similarity analysis of differentially expressed proteins in Paracoccidioides brasiliensis

1999 ◽  
Vol 37 (2) ◽  
pp. 115-121 ◽  
Author(s):  
A. F. Cunha ◽  
M. V. Sousa ◽  
S. P. Silva ◽  
R. S. A. JesuIno ◽  
C. M. A. Soares ◽  
...  
Keyword(s):  
Paracoccidioides Brasiliensis ◽  
Terminal Region ◽  
Differentially Expressed ◽  
Similarity Analysis ◽  
Differentially Expressed Proteins

Mass Spectrometry-based Label-free Quantitative Proteomic Analysis of CCl4-induced Acute Liver Injury in Mice Intervened by Total Glycosides from Ligustri Lucidi Fructus

2020 ◽  
Vol 17 ◽  
Author(s):  
Qian Lu ◽  
Hai-Zhu Xing ◽  
Nian-Yun Yang
Keyword(s):  
Insulin Resistance ◽  
Liver Injury ◽  
Protein Expression ◽  
Signaling Pathway ◽  
Proteomic Analysis ◽  
Acute Liver Injury ◽  
Liver Cells ◽  
Differentially Expressed ◽  
Liver Protein ◽  
Differentially Expressed Proteins

Background: CCl4 acute liver injury (ALI) is a classical model for experimental research. However, there are few reports involved in the fundamental research of CCl4-induced ALI Ligustri Lucidi Fructus (LLF) are and its prescription have been used to treat hepatitis illness clinically. LLF and its active ingredients displayed anti-hepatitis effects, but the mechanism of function has not been fully clarified Objective: To investigate the proteomic analysis of CCl4-induced ALI, and examine the effects of active total glycosides (TG) from LLF on ALI of mice4, including histopathological survey and proteomic changes of liver tissues, and delineate the possible underlying mechanism. Methods: CCl4 was used to produce ALI mice model. The model mice were intragastrically administrated with TG and the liver his-topathological changes of mice were examined. At the end of test, mice liver samples were collected, after protein denaturation, re-duction, desalination and enzymatic hydrolysis, identification was carried out by nano LC-ESI-OrbiTrap MS/MS technology. The data was processed by Maxquant software. The differentially-expressed proteins were screened and identified, and their biological information was also analyzed based on GO and KEGG analysis. Key protein expression was validated by Western blot analysis Results: A total of 705 differentially-expressed proteins were identified during the normal, model and administration group. 9 signifi-cant differential proteins were focused based on analysis. Liver protein expression changes of CCl4-induced ALI mice were mainly involved in several important signal channels, namely FoxO signaling pathway, autophagy-animal, insulin signaling pathway. TG has anti-liver damnification effect in ALI mice, the mechanism of which is related to FoxO1 and autophagy pathways Conclusion: CCl4 inhibited expression of insulin-Like growth factor 1 (Igf1) and 3-phosphoinositide-dependent protein kinase 1 (Pdpk1) in liver cells and induced insulin resistance, thus interfered with mitochondrial autophagy and regeneration of liver cells and the metabolism of glucose and lipid, and caused hepatic necrosis in mice. TG resisted liver injury in mice. TG adjusted the expression level of key proteins Igf1 and Pdpk1 after liver injury and improved insulin resistance, thus promoted autophagy and resisted the liver damage

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