scholarly journals Proteomic Characterization of Yersinia pestis Virulence

2005 ◽  
Vol 187 (23) ◽  
pp. 8172-8180 ◽  
Author(s):  
Brett A. Chromy ◽  
Megan W. Choi ◽  
Gloria A. Murphy ◽  
Arlene D. Gonzales ◽  
Chris H. Corzett ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Differentially Expressed ◽  
Virulence Determinants ◽  
Differentially Expressed Proteins ◽  
Mechanistic Studies ◽  
Differentially Expressed Protein ◽  
Catalase Peroxidase ◽  
Membrane Bound ◽  
Differential Gel Electrophoresis

ABSTRACT The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Caf1), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.

scholarly journals Comparative proteome analysis of the spinal dural arteriovenous fistula arterial draining vein with label-free quantitative proteomics

2020 ◽  
Author(s):  
Peixi Liu ◽  
Yuan Shi ◽  
Sichen Li ◽  
Yingjun Liu ◽  
Yingjie Zhou ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Dural Arteriovenous Fistula ◽  
Kegg Pathway ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Label Free ◽  
Spinal Dural Arteriovenous Fistula ◽  
Differentially Expressed Protein ◽  
Kegg Pathway Analysis ◽  
Go Terms

Abstract Background: Spinal dural arteriovenous fistula (SDAVF) is the most common spinal vascular shunt lesion. Although pathological changes in the SDAVF draining vein (SDAVF-DV) have been elucidated, protein changes remain enigmatic. We investigated protein changes in the SDAVF-DV.Methods: Three SDAVF-DV samples were collected, and superficial temporal artery (STA) and superficial temporal vein (STV) samples were used as controls. After quantification and enzymolysis of the proteins, label-free quantitative proteomics was performed, and the peptide mixture was fractionated and analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify the differentially expressed proteins. Bioinformatics analysis of the differentially expressed proteins was also performed using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction (PPI) network analyses.Results: Compared with the STA, the SDAVF-DV had 195 upregulated proteins and 303 downregulated proteins. GO analysis showed that the most differential GO terms in each category were the adenylate cyclase-modulating G protein-coupled receptor signalling pathway, U6 snRNP and SH3 domain binding. KEGG pathway analysis showed that the most differentially expressed protein pathway was focal adhesion. Compared with the STV, the SDAVF-DV had 158 upregulated proteins and 362 downregulated proteins. GO analysis showed that the most differential GO terms in each category were lamellipodium assembly, U6 snRNP, and SH3 domain binding. KEGG pathway analysis showed that the most differentially expressed protein pathway was dilated cardiomyopathy. The PPI analysis revealed PPIs among the top 300 proteins.Conclusions: We demonstrated that the SDAVF-DV showed specific protein expression changes under long-period venous hypertension. The results of the present study will provide insights into the pathogenesis of SDAVF formation at the protein level. The proteomic results provide a scientific foundation for further study to explore the pathophysiological mechanism of SDAVF.

Differential Proteomic Identification and Bioinformatics Analysis of Femoral Neck in Elderly Female Patients with Hyperuricaemia

2021 ◽  
Vol 18 ◽  
Author(s):  
Yingyi He ◽  
Guangming Zhang ◽  
Yuyang Huang ◽  
Qi Li ◽  
Cheng Luo
Keyword(s):  
Femoral Neck ◽  
Femoral Neck Fracture ◽  
Differentially Expressed ◽  
Control Group ◽  
Pathophysiological Mechanism ◽  
Differentially Expressed Proteins ◽  
Mineral Density ◽  
Differentially Expressed Protein ◽  
Peptide Mass ◽  
Neck Fracture

Background: Serum uric acid (UA) is positively correlated with bone mineral density (BMD). However, the mechanism by which serum UA affects BMD remains unclear. Objective: The aim was carried out to search for the functional proteins related to serum UA and femoral neck BMD to better understand the pathophysiological mechanism of osteoporosis. Materials and methods: In this study, patients in the UA group (hyperuricaemia combined with femoral neck fracture) and the control group (normal uricaemia combined with femoral neck fracture) were selected according to the inclusion criteria. Total protein was extracted from the femoral neck of each patient. Fluorescence differential gel electrophoresis was used to separate the total proteins, and the differentially expressed protein spots were detected by image analysis. After enzyme digestion, peptide mass fingerprinting and database searches were performed to identify the differentially expressed proteins. DAVID software and Kyoto Encyclopedia of Genes and Genomes (KEGG) data were used for enrichment analysis of the screened differential proteins. Results: After mass spectrometry and database searching, 66 differentially expressed protein spots were identified between the UA group and the control group. Most differentially expressed proteins functioned in cytoskeleton formation, energy metabolism, or signal transduction. They were mainly involved in 50 biological processes, including peroxisome proliferator-activated receptor (PPAR) signalling and fatty acid metabolism. PPARγ and PLIN1 were subject to Western blotting analysis detection; results were consistent with the Label-Free result. Conclusion: Based on an analysis of the biological information, these proteins may be associated with the incidence and progression of the femoral neck bone tissues of hyperuricaemia patients.

Proteomic characterization of differentially expressed proteins in breast cancer: Expression of hnRNP H1, RKIP and GRP78 is strongly associated with HER-2/neu status

2008 ◽  
Vol 2 (1) ◽  
pp. 99-107 ◽  
Author(s):  
Daohai Zhang ◽  
Lee Kian Tai ◽  
Lee Lee Wong ◽  
Thomas C. Putti ◽  
Sunil K. Sethi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Her 2

Differential Proteomic Analysis of Nasal Polyps, Chronic Sinusitis, and Normal Nasal Mucosa Tissues

2009 ◽  
Vol 141 (3) ◽  
pp. 364-368 ◽  
Author(s):  
Wu Min-man ◽  
Sun Hong ◽  
Xiao Zhi-qiang ◽  
Feng Xue-ping ◽  
Li Chang-qi ◽  
...  
Keyword(s):  
Nasal Mucosa ◽  
Nasal Polyps ◽  
Chronic Sinusitis ◽  
Response To Therapy ◽  
Differentially Expressed ◽  
Metabolic Reaction ◽  
Differentially Expressed Proteins ◽  
Cross Sectional Survey ◽  
Differentially Expressed Protein ◽  
Tof Ms

OBJECTIVE: To compare protein expression in nasal polyps, chronic sinusitis, and normal nasal mucosa tissues using proteomic technology. DESIGN: Cross-sectional survey. SUBJECTS AND METHODS: Proteins extracted from the three kinds of tissues (seven samples per group) were separated by immobilized pH4–7 gradient two-dimensional gel electrophoresis (2-DE). PDQuest software was used to analyze 2-DE images, and the differentially expressed protein spots were identified with the use of both MALDI-TOF-MS and ESI-Q-TOF-MS. Of the differentially expressed proteins, Cu/ZnSOD and PLUNC were validated with the use of immunohistochemical tests. RESULTS: High-resolution, reproducible 2-DE patterns were obtained, and 30 differentially expressed protein spots were found. The intensity of Cu/ZnSOD and PLUNC differed significantly on immunohistochemical validation. CONCLUSION: These differentially expressed proteins are involved in airway inflammatory reaction, immune response, oxidative stress, and metabolic reaction after exposure to irritants. In the future, several of these proteins may prove useful for exploring the pathogenesis of nasal polyps and chronic sinusitis or as objective biomarkers for quantitatively monitoring disease progression or response to therapy.

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