Efficient Production of Ethanol from Empty Palm Fruit Bunch Fibers by Fed-Batch Simultaneous Saccharification and Fermentation Using Saccharomyces cerevisiae

2013 ◽  
Vol 170 (8) ◽  
pp. 1807-1814 ◽  
Author(s):  
Jang Min Park ◽  
Baek-Rock Oh ◽  
Jeong-Woo Seo ◽  
Won-Kyung Hong ◽  
Anna Yu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Simultaneous Saccharification And Fermentation ◽  
Efficient Production ◽  
Fed Batch ◽  
Palm Fruit ◽  
Simultaneous Saccharification

scholarly journals Co-Production of Isobutanol and Ethanol from Prairie Grain Starch Using Engineered Saccharomyces cerevisiae

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 150
Author(s):  
Xiaodong Liu ◽  
Ebele Unaegbunam ◽  
David T. Stuart
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Simultaneous Saccharification And Fermentation ◽  
Batch Process ◽  
Energy Crop ◽  
Combustion Engines ◽  
Fed Batch ◽  
Fuel Distribution ◽  
Grain Starch ◽  
Engineered Saccharomyces Cerevisiae ◽  
Simultaneous Saccharification

Isobutanol is an important and valuable platform chemical and an appealing biofuel that is compatible with contemporary combustion engines and existing fuel distribution infrastructure. The present study aimed to compare the potential of triticale, wheat and barley starch as feedstock for isobutanol production using an engineered strain of Saccharomyces cerevisiae. A simultaneous saccharification and fermentation (SSF) approach showed that all three starches were viable feedstock for co-production of isobutanol and ethanol and could produce titres similar to that produced using purified sugar as feedstock. A fed-batch process using triticale starch yielded 0.006 g isobutanol and 0.28 g ethanol/g starch. Additionally, it is demonstrated that Fusarium graminearum infected grain starch contaminated with mycotoxin can be used as an effective feedstock for isobutanol and ethanol co-production. These findings demonstrate the potential for triticale as a purpose grown energy crop and show that mycotoxin-contaminated grain starch can be used as feedstock for isobutanol biosynthesis, thus adding value to a grain that would otherwise be of limited use.

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