scholarly journals Impact of Alkaline Pretreatment Condition on Enzymatic Hydrolysis of Sugarcane Bagasse and Pretreatment Cost

2021 ◽  
Author(s):  
Chaojun Wang ◽  
Wei Qi ◽  
Cuiyi Liang ◽  
Qiong Wang ◽  
Wen Wang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Alkaline Pretreatment ◽  
Pretreatment Condition ◽  
Hydrolysis Of

scholarly journals Impact of Alkaline Pretreatment Condition on Enzymatic Hydrolysis of Sugarcane Bagasse and Pretreatment Cost

2021 ◽  
Author(s):  
Chaojun Wang ◽  
Wei Qi ◽  
Cuiyi Liang ◽  
Qiong Wang ◽  
Wen Wang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Linear Trend ◽  
Alkaline Pretreatment ◽  
Practical Application ◽  
Severity Factor ◽  
Pretreatment Condition ◽  
Maximum Value ◽  
Combined Severity Factor ◽  
Hydrolysis Of ◽  
Temperature Time

Abstract A combined severity factor (RCSF) which is usually used to evaluate the effectiveness of hydrothermal pretreatment at above 100 ºC had been developed to assess the influence of temperature, time and alkali loading on pretreatment and enzymatic hydrolysis of lignocellulose. It is not suitable for evaluating alkaline pretreatment effectiveness at lower than 100 ºC. According to the reported deducing process, this study modified the expression of RCSF = log[CnOH- x t x e(Tr-Tb)/14.75] as RCSF = log{COH- x t x e[-13700/(Tr+273)+36.2]} which is easier and more reasonable to assess the effectiveness of alkaline pretreatment. It showed that RCSF exhibited linear trend with lignin removal, and quadratic curve relation with enzymatic hydrolysis efficiency (EHE) at the same temperature. The EHE of alkali-treated SCB could attain the maximum value at lower RCSF, which indicated that it was not necessary to continuously enhance strength of alkaline pretreatment for improving EHE. Within a certain temperature range, the alkali loading was more important than temperature and time to influence pretreatment effectiveness and EHE. Furthermore, the contribution of temperature, time and alkali loading to pretreatment cost which was seldom concerned was investigated in this work. The alkali loading contributed more than 70% to the pretreatment cost. This study laid the foundation of further optimizing alkaline pretreatment to reduce cost for its practical application.

Xylitol Production From Sugarcane Bagasse Through Ultrasound-Assisted Alkaline Pretreatment and Enzymatic Hydrolysis Followed by Fermentation

2021 ◽  
Author(s):  
Sabitri Siris Thapa ◽  
Smriti Shrestha ◽  
Muhammad Bilal Sadiq ◽  
Anil Kumar Anal
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Enzyme Concentration ◽  
Candida Guilliermondii ◽  
Alkaline Pretreatment ◽  
Xylitol Production ◽  
Suitable Alternative ◽  
Ultrasound Assisted ◽  
Xylose Production ◽  
Hydrolysis Of

Abstract This study focused on the optimization of xylitol production from sugarcane bagasse by using response surface methodology (RSM). Xylitol was produced through a series of processes, firstly, optimization of ultrasound assisted mild alkaline pretreatment for the xylan extraction from sugarcane bagasse followed by enzymatic hydrolysis of xylan to xylose by enzyme β-1,4-xylanase and finally microbial fermentation of xylose to xylitol using yeast (Candida guilliermondii), bacteria (Corynebacterium glutamicum) and their mixed culture for different time periods (0-96 h). Maximum xylan recovery of 12.059% (w/w) was observed at pretreatment; 0.73 M NaOH, 1:38.55 solid to liquid ratio and 34.77 min ultrasonication. The enzyme concentration of 400 U/g xylan at 48 h of incubation showed the highest xylose production (81.51 mg/g bagasse). Yeast (Candida guilliermondii) resulted in the highest xylitol yield (Yp/s= 0.43 g/g) after 72 h. This bioprocess route can contribute as a suitable alternative for chemical methods of xylitol production.

The Effect of Xylan Removal on the High-Solid Enzymatic Hydrolysis of Sugarcane Bagasse

2021 ◽  
Author(s):  
Leidy Patricia Quintero ◽  
Nathalia P. Q. de Souza ◽  
Adriane M. F. Milagres
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
High Solid ◽  
Hydrolysis Of

scholarly journals A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lin Dai ◽  
Tian Huang ◽  
Kankan Jiang ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Production Cost ◽  
Solid Loading ◽  
Fed Batch ◽  
Acid Pretreatment ◽  
Furoic Acid ◽  
Cooling Crystallization ◽  
Mineral Acids ◽  
Hydrolysis Of

Abstract Background Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 °C for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization. Conclusion This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

Enhanced enzymatic hydrolysis of sugarcane bagasse with ferric chloride pretreatment and surfactant

2017 ◽  
Vol 229 ◽  
pp. 96-103 ◽  
Author(s):  
Hongdan Zhang ◽  
Guangying Ye ◽  
Yutuo Wei ◽  
Xin Li ◽  
Aiping Zhang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Ferric Chloride ◽  
Hydrolysis Of

scholarly journals A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

2020 ◽  
Author(s):  
Lin Dai ◽  
Tian Huang ◽  
Kankan Jiang ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Production Cost ◽  
Solid Loading ◽  
Fed Batch ◽  
Acid Pretreatment ◽  
Furoic Acid ◽  
Cooling Crystallization ◽  
Mineral Acids ◽  
Hydrolysis Of

Abstract Background: Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results: The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 oC for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization.Conclusion: This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

scholarly journals Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Robinson Timung ◽  
Narendra Naik Deshavath ◽  
Vaibhav V. Goud ◽  
Venkata V. Dasu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Crystallinity Index ◽  
Sem Analysis ◽  
Reducing Sugar ◽  
Dilute Acid Pretreatment ◽  
Crystalline Cellulose ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

Sign in / Sign up

Export Citation Format

Share Document