scholarly journals Inter- and Intrapopulational Heterogeneity of Characteristic Markers in Adult Human Neural Crest-derived Stem Cells

2021 ◽  
Author(s):  
Beatrice A. Windmöller ◽  
Anna L. Höving ◽  
Cornelius Knabbe ◽  
Johannes F. W. Greiner
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Genetic Heterogeneity ◽  
Single Cells ◽  
Inferior Turbinate ◽  
Dependent Manner ◽  
Developmental Potential ◽  
Adult Human ◽  
Marker Proteins ◽  
Heterogeneous Expression

Abstract Adult human neural crest-derived stem cells (NCSCs) are found in a variety of adult tissues and show an extraordinary broad developmental potential. Despite their great differentiation capacity, increasing evidence suggest a remaining niche-dependent variability between different NCSC-populations regarding their differentiation behavior and expression signatures. In the present study, we extended the view on heterogeneity of NCSCs by identifying heterogeneous expression levels and protein amounts of characteristic markers even between NCSCs from the same niche of origin. In particular, populations of neural crest-derived inferior turbinate stem cells (ITSCs) isolated from different individuals showed significant variations in characteristic NCSC marker proteins Nestin, S100 and Slug in a donor-dependent manner. Notably, increased nuclear protein amounts of Slug were accompanied by a significantly elevated level of nuclear NF-κB-p65 protein, suggesting an NF-κB-dependent regulation of NCSC-makers. In addition to this interpopulational genetic heterogeneity of ITSC-populations from different donors, single ITSCs also revealed a strong heterogeneity regarding the protein amounts of Nestin, S100, Slug and NF-κB-p65 even within the same clonal culture. Our present findings therefor strongly suggest ITSC-heterogeneity to be at least partly based on an interpopulational genetic heterogeneity dependent on the donor accompanied by a stochastic intrapopulational heterogeneity between single cells. We propose this stochastic intrapopulational heterogeneity to occur in addition to the already described genetic variability between clonal NCSC-cultures and the niche-dependent plasticity of NCSCs. Our observations offer a novel perspective on NCSC-heterogeneity, which may build the basis to understand heterogeneous NCSC-behavior. Graphical Abstract

scholarly journals Inter- and Intrapopulational Heterogeneity of Characteristic Markers in Adult Human Neural Crest-Derived Stem Cells

2021 ◽  
Author(s):  
Beatrice A. Windmöller ◽  
Anna L. Höving ◽  
Johannes F.W. Greiner
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Genetic Heterogeneity ◽  
Single Cells ◽  
Inferior Turbinate ◽  
Dependent Manner ◽  
Developmental Potential ◽  
Adult Human ◽  
Marker Proteins ◽  
Heterogeneous Expression

Abstract Adult human neural crest-derived stem cells (NCSCs) are found in a variety of adult tissues and show an extraordinary broad developmental potential. NCSCs are therefore promising candidates for applications in regenerative medicine, although increasing evidence suggest a remaining nichedependent variability between different NCSC-populations regarding their behavior and expression signatures. In the present study, we extended the view on heterogeneity of NCSCs by identifying heterogeneous expression levels and protein amounts of characteristic markers even between NCSCs from the same niche of origin. In particular, populations of neural crest-derived inferior turbinate stem cells (ITSCs) isolated from different individuals showed significant variations in characteristic NCSC marker proteins Nestin, S100 and Slug in a donor-dependent manner. Notably, increased nuclear protein amounts of Slug were accompanied by a significantly elevated level of nuclear NF-κB-p65 protein, suggesting an NF-κB-dependent regulation of NCSC-makers. In addition to this interpopulational genetic heterogeneity of ITSC-populations from different donors, single ITSCs also revealed a strong heterogeneity regarding the protein amounts of Nestin, S100, Slug and NF-κB-p65 even within the same clonal culture. Our present findings therefor strongly suggest ITSC-heterogeneity to be at least partly based on an interpopulational genetic heterogeneity dependent on the donor accompanied by a stochastic intrapopulational heterogeneity between single cells. We propose this stochastic intrapopulational heterogeneity to occur in addition to the already described genetic variability between clonal NCSC-cultures and the niche-dependent plasticity of NCSCs. Our observations offer a novel perspective on NCSC-heterogeneity, which may build the basis to understand heterogeneous NCSC-behavior and improve their clinical applicability.

scholarly journals Inter- and intrapopulational heterogeneity of characteristic markers in adult human neural crest-derived stem cells

2021 ◽  
Author(s):  
Beatrice A Windmoeller ◽  
Anna Hoeving ◽  
Johannes F.W. Greiner
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Genetic Heterogeneity ◽  
Single Cells ◽  
Inferior Turbinate ◽  
Dependent Manner ◽  
Developmental Potential ◽  
Adult Human ◽  
Marker Proteins ◽  
Heterogeneous Expression

Adult human neural crest-derived stem cells (NCSCs) are found in a variety of adult tissues and show an extraordinary broad developmental potential. NCSCs are therefore promising candidates for applications in regenerative medicine, although increasing evidence suggest a remaining niche-dependent variability between different NCSC-populations regarding their behavior and expression signatures. In the present study, we extended the view on heterogeneity of NCSCs by identifying heterogeneous expression levels and protein amounts of characteristic markers even between NCSCs from the same niche of origin. In particular, populations of neural crest-derived inferior turbinate stem cells (ITSCs) isolated from different individuals showed significant variations in characteristic NCSC marker proteins Nestin, S100 and Slug in a donor-dependent manner. Notably, increased nuclear protein amounts of Slug were accompanied by a significantly elevated level of nuclear NF-κB-p65 protein, suggesting an NF-κB-dependent regulation of NCSC-makers. In addition to this interpopulational genetic heterogeneity of ITSC-populations from different donors, single ITSCs also revealed a strong heterogeneity regarding the protein amounts of Nestin, S100, Slug and NF-κB-p65 even within the same clonal culture. Our present findings therefor strongly suggest ITSC-heterogeneity to be at least partly based on an interpopulational genetic heterogeneity dependent on the donor accompanied by a stochastic intrapopulational heterogeneity between single cells. We propose this stochastic intrapopulational heterogeneity to occur in addition to the already described genetic variability between clonal NCSC-cultures and the niche-dependent plasticity of NCSCs. Our observations offer a novel perspective on NCSC-heterogeneity, which may build the basis to understand heterogeneous NCSC-behavior and improve their clinical applicability.

scholarly journals Isolation of Novel Multipotent Neural Crest-Derived Stem Cells from Adult Human Inferior Turbinate

2012 ◽  
Vol 21 (5) ◽  
pp. 742-756 ◽  
Author(s):  
Stefan Hauser ◽  
Darius Widera ◽  
Firas Qunneis ◽  
Janine Müller ◽  
Christin Zander ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Inferior Turbinate ◽  
Adult Human

Neural crest stem cells are also present is adult human bone marrow and adipose tissue

Cytotherapy ◽  
2015 ◽  
Vol 17 (6) ◽  
pp. S37
Author(s):  
Cécile Coste ◽  
Virginie Neirinckx ◽  
Anil Sharma ◽  
Bernard Rogister ◽  
François Lallemend ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Neural Crest ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Neural Crest Stem Cells ◽  
Adult Human

scholarly journals Sexual dimorphisms in adult human neural, mesoderm‐derived, and neural crest‐derived stem cells

FEBS Letters ◽  
2019 ◽  
Vol 593 (23) ◽  
pp. 3338-3352 ◽  
Author(s):  
Johannes F.W. Greiner ◽  
Madlen Merten ◽  
Christian Kaltschmidt ◽  
Barbara Kaltschmidt
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Sexual Dimorphisms ◽  
Adult Human

scholarly journals Blood Serum Stimulates p38-Mediated Proliferation and Changes in Global Gene Expression of Adult Human Cardiac Stem Cells

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1472 ◽  
Author(s):  
Anna L. Höving ◽  
Kazuko E. Schmidt ◽  
Madlen Merten ◽  
Jassin Hamidi ◽  
Ann-Katrin Rott ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
P38 Mapk ◽  
Regulatory Networks ◽  
Human Blood Plasma ◽  
Cardiac Stem Cells ◽  
Dependent Manner ◽  
Protective Effects ◽  
Adult Human ◽  
Age And Sex

During aging, senescent cells accumulate in various tissues accompanied by decreased regenerative capacities of quiescent stem cells, resulting in deteriorated organ function and overall degeneration. In this regard, the adult human heart with a generally low regenerative potential is of extreme interest as a target for rejuvenating strategies with blood borne factors that might be able to activate endogenous stem cell populations. Here, we investigated for the first time the effects of human blood plasma and serum on adult human cardiac stem cells (hCSCs) and showed significantly increased proliferation capacities and metabolism accompanied by a significant decrease of senescent cells, demonstrating a beneficial serum-mediated effect that seemed to be independent of age and sex. However, RNA-seq analysis of serum-treated hCSCs revealed profound effects on gene expression depending on the age and sex of the plasma donor. We further successfully identified key pathways that are affected by serum treatment with p38-MAPK playing a regulatory role in protection from senescence and in the promotion of proliferation in a serum-dependent manner. Inhibition of p38-MAPK resulted in a decline of these serum-mediated beneficial effects on hCSCs in terms of decreased proliferation and accelerated senescence. In summary, we provide new insights in the regulatory networks behind serum-mediated protective effects on adult human cardiac stem cells.

scholarly journals Intrastriatal Transplantation of Adult Human Neural Crest-Derived Stem Cells Improves Functional Outcome in Parkinsonian Rats

2014 ◽  
Vol 4 (1) ◽  
pp. 31-43 ◽  
Author(s):  
Janine Müller ◽  
Christiana Ossig ◽  
Johannes F.W. Greiner ◽  
Stefan Hauser ◽  
Mareike Fauser ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Functional Outcome ◽  
Adult Human

scholarly journals Neural Crest Stem Cells from Dental Tissues: A New Hope for Dental and Neural Regeneration

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Gaskon Ibarretxe ◽  
Olatz Crende ◽  
Maitane Aurrekoetxea ◽  
Victoria García-Murga ◽  
Javier Etxaniz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Therapy ◽  
Neural Crest ◽  
Neural Regeneration ◽  
Dental Tissues ◽  
Adult Human ◽  
Regenerative Dentistry ◽  
Cord Injury

Several stem cell sources persist in the adult human body, which opens the doors to both allogeneic and autologous cell therapies. Tooth tissues have proven to be a surprisingly rich and accessible source of neural crest-derived ectomesenchymal stem cells (EMSCs), which may be employed to repair disease-affected oral tissues in advanced regenerative dentistry. Additionally, one area of medicine that demands intensive research on new sources of stem cells is nervous system regeneration, since this constitutes a therapeutic hope for patients affected by highly invalidating conditions such as spinal cord injury, stroke, or neurodegenerative diseases. However, endogenous adult sources of neural stem cells present major drawbacks, such as their scarcity and complicated obtention. In this context, EMSCs from dental tissues emerge as good alternative candidates, since they are preserved in adult human individuals, and retain both high proliferation ability and a neural-like phenotypein vitro. In this paper, we discuss some important aspects of tissue regeneration by cell therapy and point out some advantages that EMSCs provide for dental and neural regeneration. We will finally review some of the latest research featuring experimental approaches and benefits of dental stem cell therapy.

Sign in / Sign up

Export Citation Format

Share Document