Background:
This study determined the effect of Biochanin A (BCA) on isoproterenol
(ISO) induced Myocardial Infarction (MI) in male Wistar rats.
Methods:
Animals (weighing 150-180 g) were divided into four groups, with six animals in each
group and pretreated with BCA (10mg/kg Body Weight [BW]) and ɑ-tocopherol (60mg/kg BW) for
30 days; and ISO (20mg/kg BW) was administrated subcutaneously on the 31st and 32nd day.
Results:
ISO-induced MI rats demonstrated the significant elevation of serum glutamic oxaloacetic
transaminase, serum glutamic pyruvic transaminase, lactate dehydrogenase, creatine kinase-MB and
cardiac troponin; however, concomitant pretreatment with BCA protected the rats from cardiotoxicity
caused by ISO. Activities of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione
peroxidase, glutathione-S-transferase and glutathione reductase significantly reduced in the heart
with ISO-induced MI. Pretreatment with BCA produced a marked reversal of these antioxidant
enzymes related to MI-induced by ISO.
Conclusion:
In conclusion, this study suggested that BCA exerts cardioprotective effects through
modulating lipid peroxidation, enhancing antioxidants, and detoxifying enzyme systems.
Screening dietary biochanin A, daidzein, equol and genistein for their potential to increase DHA biosynthesis in rainbow trout (Oncorhynchus mykiss)
