scholarly journals CCN3/NOV small interfering RNA enhances fibrogenic gene expression in primary hepatic stellate cells and cirrhotic fat storing cell line CFSC

2011 ◽  
Vol 6 (1) ◽  
pp. 11-25 ◽  
Author(s):  
Erawan Borkham-Kamphorst ◽  
Claudia R. van Roeyen ◽  
Eddy Van de Leur ◽  
Jürgen Floege ◽  
Ralf Weiskirchen
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Hepatic Stellate Cells ◽  
Small Interfering Rna ◽  
Stellate Cells ◽  
Interfering Rna

The Delivery of Small Interfering RNA to Hepatic Stellate Cells Using a Lipid Nanoparticle Composed of a Vitamin A-Scaffold Lipid-Like Material

2017 ◽  
Vol 106 (8) ◽  
pp. 2046-2052 ◽  
Author(s):  
Naoyuki Toriyabe ◽  
Yu Sakurai ◽  
Akari Kato ◽  
Shoshiro Yamamoto ◽  
Kota Tange ◽  
...  
Keyword(s):  
Vitamin A ◽  
Hepatic Stellate Cells ◽  
Small Interfering Rna ◽  
Stellate Cells ◽  
Lipid Nanoparticle ◽  
Interfering Rna

scholarly journals Specific inhibition of gene expression using a stably integrated, inducible small‐interfering‐RNA vector

EMBO Reports ◽  
2003 ◽  
Vol 4 (6) ◽  
pp. 609-615 ◽  
Author(s):  
Marc van de Wetering ◽  
Irma Oving ◽  
Vanesa Muncan ◽  
Menno Tjon Pon Fong ◽  
Helen Brantjes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Small Interfering Rna ◽  
Specific Inhibition ◽  
Interfering Rna

scholarly journals Synaptotagmin VII Is Targeted to Dense-core Vesicles and Regulates Their Ca2+-dependent Exocytosis in PC12 Cells

2004 ◽  
Vol 279 (50) ◽  
pp. 52677-52684 ◽  
Author(s):  
Mitsunori Fukuda ◽  
Eiko Kanno ◽  
Megumi Satoh ◽  
Chika Saegusa ◽  
Akitsugu Yamamoto
Keyword(s):  
Plasma Membrane ◽  
Neuropeptide Y ◽  
Cell Line ◽  
Pc12 Cells ◽  
Pc12 Cell ◽  
Small Interfering Rna ◽  
Dense Core ◽  
Dense Core Vesicles ◽  
Pc12 Cell Line ◽  
Interfering Rna

It has recently been proposed that synaptotagmin (Syt) VII functions as a plasma membrane Ca2+sensor for dense-core vesicle exocytosis in PC12 cells based on the results of transient overexpression studies using green fluorescent protein (GFP)-tagged Syt VII; however, the precise subcellular localization of Syt VII is still a matter of controversy (plasma membraneversussecretory granules). In this study we established a PC12 cell line “stably expressing” the Syt VII-GFP molecule and demonstrated by immunocytochemical and immunoelectron microscopic analyses that the Syt VII-GFP protein is localized on dense-core vesicles as well as in other intracellular membranous structures, such as thetrans-Golgi network and lysosomes. Syt VII-GFP forms a complex with endogenous Syts I and IX, but not with Syt IV, and it colocalize well with Syts I and IX in the cellular processes (where dense-core vesicles are accumulated) in the PC12 cell line. We further demonstrated by an N-terminal antibody-uptake experiment that Syt VII-GFP-containing dense-core vesicles undergo Ca2+-dependent exocytosis, the same as endogenous Syt IX-containing vesicles. Moreover, silencing of Syt VII-GFP with specific small interfering RNA dramatically reduced high KCl-dependent neuropeptide Y secretion from the stable PC12 cell line (∼60% of the control cells), whereas the same small interfering RNA had little effect on neuropeptide Y secretion from the wild-type PC12 cells (∼85–90% of the control cells), indicating that the level of endogenous expression of Syt VII molecules must be low. Our results indicate that the targeting of Syt VII-GFP molecules to specific membrane compartment(s) is affected by the transfection method (transient expressionversusstable expression) and suggested that Syt VII molecule on dense-core vesicles functions as a vesicular Ca2+sensor for exocytosis in endocrine cells.

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