Real-Time Recombinase Polymerase Amplification Assay for the Detection of Vibrio cholerae in Seafood

2017 ◽  
Vol 10 (8) ◽  
pp. 2657-2666
Author(s):  
Yuyi Tang ◽  
Yunqing Cao ◽  
Yongxin Yu ◽  
Shiqiang Yan ◽  
Yongjie Wang ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Real Time ◽  
Recombinase Polymerase Amplification ◽  
Amplification Assay ◽  
Recombinase Polymerase Amplification Assay

scholarly journals Rapid identification of quarantine invasiveSolanum elaeagnifoliumby real-time, isothermal recombinase polymerase amplification assay

RSC Advances ◽  
2017 ◽  
Vol 7 (83) ◽  
pp. 52573-52580 ◽  
Author(s):  
Rong Lei ◽  
Zhengyue Yan ◽  
Fan Hu ◽  
Shuifang Zhu ◽  
Yufen Xiong ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Identification ◽  
Recombinase Polymerase Amplification ◽  
Amplification Assay ◽  
Solanum Elaeagnifolium ◽  
Recombinase Polymerase Amplification Assay ◽  
Implement Strategy

An easy-to-implement strategy to identifySolanum elaeagnifoliumby utilizing recombinase polymerase amplification (RPA) technology was developed.

scholarly journals Rapid molecular detection of Zika virus in urine using the recombinase polymerase amplification assay

2016 ◽  
Author(s):  
Ahmed Abd El Wahed ◽  
Sabri S. Sanabani ◽  
Oumar Faye ◽  
Rodrigo Pessôa ◽  
João Veras Patriota ◽  
...  
Keyword(s):  
Real Time ◽  
Zika Virus ◽  
Recombinase Polymerase Amplification ◽  
Control Measures ◽  
Mosquito Vector ◽  
Rt Pcr ◽  
Low Resource Settings ◽  
Low Resource ◽  
Amplification Assay ◽  
Recombinase Polymerase Amplification Assay

AbstractBackgroundCurrently the detection of Zika virus (ZIKV) in patient samples is done by real-time RT-PCR. Samples collected from rural area are sent to highly equipped laboratories for screening. A rapid point-of-care test is needed to detect the virus, especially at low resource settings.Methodology/Principal FindingsIn this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes). No cross-reactivity was detected to other flaviviruses, alphaviruses and arboviruses. Compared to real-time RT-PCR, the diagnostic sensitivity was 92%, while the specificity was 100%.Conclusions/SignificanceThe developed assay is a promising platform for rapid point of need detection of ZIKV in low resource settings and elsewhere (e.g. during mass gathering).Author SummaryCurrently, Dengue (DENV), Zika (ZIKV) and Chikungunya (CHIKV) viruses represent a global threat. The clinical picture of the acute febrile diseases caused by DENV, ZIKV and CHIKV is very similar, in addition, the same mosquito vector is involved in the transmission cycle. The differentiation between them is of great importance as supportive treatment differs and the identification of any of the three viruses prompts implementation of control measures to avoid spreading of an outbreak. We have developed an assay for the detection of ZIKV genome. The assay based on isothermal “recombinase polymerase amplification” assay, which was performed at one temperature (42°C). The result was obtained in maximum of 15 minutes. Moreover, the assay is easy to be implemented at low resource settings.

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