Meat Species Identification: Amplification Refractory Mutation System-Polymerase Chain Reaction–Based Assay

2019 ◽  
Vol 12 (12) ◽  
pp. 2813-2822 ◽  
Author(s):  
Muhammad Waqas ◽  
Zahid Hussain ◽  
Awais Ihsan
Keyword(s):  
Polymerase Chain Reaction ◽  
Species Identification ◽  
Amplification Refractory Mutation System ◽  
Chain Reaction ◽  
Meat Species Identification ◽  
Mutation System ◽  
Meat Species ◽  
Polymerase Chain

scholarly journals Polymerase chain reaction in meat species identification

Meso ◽  
2021 ◽  
Vol 23 (6) ◽  
pp. 514-522
Author(s):  
Dora Zurak ◽  
Kristina Kljak ◽  
Željka Cvrtila
Keyword(s):  
Polymerase Chain Reaction ◽  
Species Identification ◽  
Chain Reaction ◽  
Meat Species Identification ◽  
Meat Species ◽  
Polymerase Chain ◽  
Dna Pcr

Lebensmittel gelten als authentisch, wenn das Produkt oder sein Inhalt mit dem Originalzustand und den Angaben auf der Deklaration übereinstimmt. Abweichungen von den Behauptungen und Angaben auf der Deklaration werden als Verstöße gegen die Lebensmittelvorschriften angesehen. Aufgrund des gestiegenen Bewusstseins für das Problem und die negativen Auswirkungen von Lebensmittelbetrug wird der Fleischkonsum maßgeblich von der Wahrnehmung der Verbraucher in Bezug auf die Lebensmittelqualität und Sicherheit beeinflusst. Daher ist eine genaue Etikettierung einer der wichtigsten Faktoren, die die Verbraucherpräferenzen bei der Auswahl und dem Kauf von Fleisch und Fleischerzeugnissen beeinflussen. Aus diesem Grund sind Analysemethoden zur Überprüfung der Echtheit von Fleisch und Fleischerzeugnissen wichtig, um die Produktqualität, Lebensmittelsicherheit und den Verbraucherschutz zu gewährleisten. Die Substitution von Fleischsorten ist nicht das einzige Kriterium für die Bestimmung der Echtheit von Fleisch und Fleischerzeugnissen, sondern auch die Herkunft des Fleisches, die Behandlung des Fleisches und der Zusatz von sonstigen Zutaten. Die Polymerase-Kettenreaktion (PCR) und die davon abgeleiteten Technologien haben sich als die am besten geeigneten Methoden zur Identifizierung von Arten in rohem und technologisch verarbeitetem Fleisch erwiesen. Die PCR-Methoden basieren hauptsächlich auf der Identifizierung der Zielregion der mitochondrialen DNA, was den Nachweis von Arten in einer breiten Palette von Fleischerzeugnissen ermöglicht, einschließlich aller Haustiere und des für den menschlichen Verzehr bestimmten Wildfleischs. Sie haben jedoch auch einige Nachteile. So eignet sich beispielsweise die zufällig amplifizierte polymorphe DNA (PCR-RAPD) nicht für den Artennachweis in Fleischmischungen und in thermisch verarbeitetem Fleisch. Andererseits sind einige Methoden teuer und zeitaufwendig, und die Ergebnisse sind schwer zu interpretieren. In diesem Artikel werden die wichtigsten PCR-basierten Methoden zur Identifizierung von Fleischarten vorgestellt und beschrieben.

scholarly journals CCL5 rs2107538 Polymorphism Increased the Risk of Tuberculosis in a Sample of Iranian Population

2016 ◽  
Vol 117 (2-3) ◽  
pp. 90-97 ◽  
Author(s):  
Hamid Reza Kouhpayeh ◽  
Mohsen Taheri ◽  
Mana Baziboroon ◽  
Mohammad Naderi ◽  
Gholamreza Bahari ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pulmonary Tuberculosis ◽  
Chemokine Receptor ◽  
Iranian Population ◽  
Amplification Refractory Mutation System ◽  
Chain Reaction ◽  
Mutation System ◽  
Chemokine Ligand ◽  
Allele Specific ◽  
Polymerase Chain

Cysteine-cysteine chemokine ligand 5 (CCL5) with immunoregulatory and inflammatory activities has an important role in granuloma formations that activates and stimulates T-cells and macrophages. Cysteine-cysteine chemokine receptor 5 (CCR5) is a chemokine receptor, which is important for migration of immune cells to site of infection. In the present study we investigated the possible association between CCL5 –403G/A (rs2107538), CCL5 –28C/G (rs2280788) and CCR5 Δ32 polymorphisms and pulmonary tuberculosis (PTB) in an Iranian population. This case-control study was performed on 160 patients with pulmonary tuberculosis and 160 unrelated healthy subjects. The CCL5 –403G/A, CCL5 –28C/G and CCR5 Δ32 polymorphisms were genotyped by allele-specific polymerase chain reaction (AS-PCR), tetra amplification refractory mutation system polymerase chain reaction (T-ARMS PCR) and PCR, respectively. Our results showed that GA as well as GA+AA genotypes of CCL5 –403G/A (rs2107538) increased the risk of PTB in comparison with GG genotype (OR=1.70, 95% CI=1.03–2.81, P=0.038 and OR=1.64, 95% CI=1.00–2.68, P=0.049, respectively). No significant association was found between CCL5 –28C/G as well as CCR5 Δ32 polymorphism and PTB risk. In conclusion, our findings proposed that CCL5 –403G>A polymorphism may be a risk factor for susceptibility to PTB in our population. Larger sample sizes with different ethnicities are required to validate our findings.

scholarly journals Molecular Authentication of Dendrobium Species by Multiplex Polymerase Chain Reaction and Amplification Refractory Mutation System Analysis

2012 ◽  
Vol 137 (6) ◽  
pp. 438-444 ◽  
Author(s):  
Chu-Hui Chiang ◽  
Tsong-Ann Yu ◽  
Shu-Fang Lo ◽  
Chao-Lin Kuo ◽  
Wen-Huang Peng ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
System Analysis ◽  
Pcr Amplification ◽  
Amplification Refractory Mutation System ◽  
Chain Reaction ◽  
5.8S Rdna ◽  
Traditional Chinese Herbal Medicine ◽  
Mutation System ◽  
Polymerase Chain

The genus Dendrobium is important in traditional Chinese herbal medicine, and the precise identification of Dendrobium species is critical for the treatment and for pharmacological research. In the present study, a ribosomal DNA (rDNA) internal transcribed spacer (ITS) region-based analysis was used to ascertain the phylogenetic relationship among 20 Dendrobium species. The lengths of the ITS regions among the 20 species ranged from 636 to 653 bp, and the identities of the rDNA regions among the different species ranged from 75.7% to 99.1%. The results also showed that the ITS1 and ITS2 regions exhibit more variation than the 5.8S rDNA. A phylogenetic tree derived from the ITS sequence indicated that six medicinal Dendrobium species, of which five are common medicinal plants in the Taiwan market, were closely related and shared a common clade. Multiplex polymerase chain reaction (PCR) amplification was successfully performed to identify the six medicinal Dendrobium species, and amplification refractory mutation system (ARMS) PCR was used to distinguish D. tosaense specifically from the 19 other Dendrobium species. The established PCR-based (multiplex and ARMS) analyses can be used for the authentication of the raw materials of medicinal Dendrobium from other species.

scholarly journals A quantitative polymerase chain reaction assay for the detection of equine (horse and donkey)-originated meat in processed bovine meat products

2018 ◽  
Vol 25 (1) ◽  
pp. 38-46
Author(s):  
Aysun Türkanoǧlu Özçelik ◽  
Semiramis Yılmaz ◽  
Sevda Gökbora ◽  
Mehmet İnan
Keyword(s):  
Polymerase Chain Reaction ◽  
Species Identification ◽  
Quantitative Polymerase Chain Reaction ◽  
Meat Products ◽  
Melting Curve Analysis ◽  
Chain Reaction ◽  
Quantification Analysis ◽  
Meat Species ◽  
Polymerase Chain ◽  
Identification And Quantification

Meat is one of the most important basic foodstuffs in human nutrition. Nowadays, adulteration and authenticity are common problems for meat products. Identification of meat species is important in terms of consumer protection and prevention of adulteration. There are different methods to determine adulteration of meat and meat products. These methods are histological controls, serological tests, and quantitative polymerase chain reaction. In this study, species identification and quantification analysis of meat and meat products were done by using horse-, donkey-, and bovine-specific primers with quantitative polymerase chain reaction method. Triple meat mixtures containing horse and donkey meat ranging from 0.1 to 50% levels were prepared within a bovine mixture for using species identification and quantification analysis. The method specificity was confirmed by melting curve analysis. In conclusion, quantitative polymerase chain reaction is an easy, rapid, and reliable method for meat species identification, and with this study an applicable method was developed for the detection and quantification of equine-originated meat in bovine meat products.

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