Effect of PGRs in adventitious root culture in vitro: present scenario and future prospects

2015 ◽  
Vol 26 (3) ◽  
pp. 307-321 ◽  
Author(s):  
Naseem Ahmad ◽  
Nigar Fatima ◽  
Iqbal Ahmad ◽  
Mohammad Anis
Keyword(s):  
Adventitious Root ◽  
Root Culture ◽  
Future Prospects ◽  
Culture In Vitro ◽  
Adventitious Root Culture

scholarly journals Establishment of In Vitro Adventitious Root Cultures and Analysis of Andrographolide in Andrographis Paniculata

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800
Author(s):  
Shiv Narayan Sharma ◽  
Zenu Jha ◽  
Rakesh Kumar Sinha
Keyword(s):  
Adventitious Root ◽  
Adventitious Roots ◽  
Leaf Explants ◽  
Root Culture ◽  
Andrographis Paniculata ◽  
Ms Medium ◽  
Plant Parts ◽  
Bioactive Component ◽  
Adventitious Root Culture

Andrographolide is the principal bioactive component of the medicinal plant Andrographis paniculata, to which various diverse pharmacological properties are attributed. Traditionally, andrographolide was extracted from the leaves, stems and other parts of the plant. Leaves have the highest andrographolide content (2–3%) in comparison with the other plant parts. Adventitious root culture of leaf explants of A. paniculata was studied using different strength MS medium supplemented by different concentrations of auxins and a combination of NAA + kinetin for growth and andrographolide production. Among the different auxin treatments in adventitious root culture, only NAA was able to induce adventitious roots. Adventitious roots grown in modified strength MS medium showed the highest root growth (26.7±1.52), as well as the highest amount of andrographolide (133.3±1.5 mg/g DW) as compared with roots grown in half-and full-strength MS medium. Growth kinetics showed maximum biomass production after five weeks of culture in different strength MS liquid medium. The produced andrographolide content was 3.5- 5.5 folds higher than that of the natural plant, depending on the medium strength.

scholarly journals Feasible Production of Lignans and Neolignans in Root-Derived In Vitro Cultures of Flax (Linum usitatissimum L.)

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 409
Author(s):  
Sumaira Anjum ◽  
Amna Komal ◽  
Samantha Drouet ◽  
Humera Kausar ◽  
Christophe Hano ◽  
...  
Keyword(s):  
Callus Culture ◽  
Adventitious Root ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Root Culture ◽  
In Vitro Cultures ◽  
Total Phenolic ◽  
Adventitious Root Culture

Flax lignans and neolignans impart health benefits, particularly in treating different types of cancers, due to their strong phytoestrogenic and antioxidant properties. The present study enhances the comprehension on the biosynthesis of antioxidant lignans and neolignans in root-derived in vitro cultures of flax (both callus and adventitious root). The results presented here clearly showed that the adventitious root culture efficiently produced a higher amount of lignans (at day 40) and neolignans (at day 30) than callus culture of flax. High performance liquid chromatography (HPLC) analysis revealed that the accumulations of secoisolariciresinol diglucoside (SDG, 5.5 mg g−1 DW (dry weight)) and dehydrodiconiferyl alcohol glucoside (DCG, 21.6 mg/g DW) were 2-fold higher, while guaiacylglycerol-β-coniferyl alcohol ether glucoside (GGCG, 4.9 mg/g DW) and lariciresinol glucoside (LDG, 11.9 mg/g DW) contents were 1.5-fold higher in adventitious root culture than in callus culture. Furthermore, the highest level of total phenolic production (119.01 mg/L), with an antioxidant free radical scavenging activity of 91.01%, was found in adventitious root culture at day 40, while the maximum level of total flavonoid production (45.51 mg/L) was observed in callus culture at day 30 of growth dynamics. These results suggest that adventitious root culture can be a good candidate for scaling up to industrial level to commercially produce these pharmacologically and nutritionally valuable metabolites.

scholarly journals Establishment of In vitro Adventitious Root Cultures and Analysis of Flavonoids in Rumex crispus

2015 ◽  
Vol 25 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Majid Mahdieh ◽  
Mitra Noori ◽  
Simin Hoseinkhani
Keyword(s):  
Adventitious Root ◽  
Adventitious Roots ◽  
Culture Media ◽  
Leaf Explants ◽  
Root Culture ◽  
Root Extract ◽  
Rumex Crispus ◽  
Adventitious Root Culture ◽  
Flavonoid Composition

Adventitious root culture of leaf explants of R. crispus was established using different MS supplemented with different concentrations of auxins and a combination of NAA and Kn for growth and flavonoids production. Among the different auxins, NAA was more effective than IAA to induce adventitious roots. Adventitious roots grown on MS containing 5 ?M NAA and 0.5 ?M Kn showed the highest root growth, as well as the highest amount of total flavonoids (= 6) as compared with roots grown in other media. Chromatographic purification of the root extract showed that flavonoid composition also was influenced by hormone combinations in the culture media. The addition of Kn to the medium reduced or suppressed myricetin (M) and naringenin (N) production. Quercetin (Q) was not found in media containing Kn alone similar to the control medium. Isorhamnetin (I), kaempferol (K) and rutin (R) were produced in the roots on media supplemented with all hormone combinations, but were absent in 0.1 ?M Kn supplemented media similar to the control roots.Plant Tissue Cult. & Biotech. 25(1): 63-70, 2015 (June)

Adventitious root culture of Pfaffia glomerata (Spreng.) Pedersen in a roller bottle system: An alternative source of β-ecdysone

2021 ◽  
Vol 43 ◽  
pp. 1-7
Author(s):  
Thaila Fernanda Oliveira da Silva ◽  
Cristina Sayuri Yamaguchi ◽  
Susana Tavares Cotrim Ribeiro ◽  
Alexandre da Silva Avincola ◽  
Eduardo Jorge Pilau ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Root Culture ◽  
Roller Bottle ◽  
Alternative Source ◽  
Pfaffia Glomerata ◽  
Adventitious Root Culture

Factors influencing podophyllotoxin production in adventitious root culture of Podophyllum hexandrum Royle

2014 ◽  
Vol 36 (4) ◽  
pp. 1009-1021 ◽  
Author(s):  
Manoharan Rajesh ◽  
Ganeshan Sivanandhan ◽  
Muthukrishnan Arun ◽  
Venkatachalam Vasudevan ◽  
Jeevaraj Theboral ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Root Culture ◽  
Podophyllum Hexandrum ◽  
Factors Influencing ◽  
Adventitious Root Culture

Production of secoiridoids by adventitious root culture of Swertia japonica

2015 ◽  
Vol 51 (5) ◽  
pp. 524-529 ◽  
Author(s):  
Hiroko Kawakami ◽  
Kojiro Hara ◽  
Masashi Komine ◽  
Yoshikazu Yamamoto
Keyword(s):  
Adventitious Root ◽  
Root Culture ◽  
Adventitious Root Culture ◽  
Swertia Japonica

scholarly journals Biomass and Flavonoid Production of Gynura procumbens (L.). Merr Adventitious Root Culture in Baloon-type Bubble-bioreactor Influenced by Elicitation

2018 ◽  
Vol 17 (2) ◽  
pp. 107-119 ◽  
Author(s):  
Hanik Faizah ◽  
Mulyadi Tanjung ◽  
Hery Purnobasuk ◽  
Yosephine Sri Wulan
Keyword(s):  
Adventitious Root ◽  
Root Culture ◽  
Adventitious Root Culture
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