Effect of PGRs in adventitious root culture in vitro: present scenario and future prospects

Andrographolide is the principal bioactive component of the medicinal plant Andrographis paniculata, to which various diverse pharmacological properties are attributed. Traditionally, andrographolide was extracted from the leaves, stems and other parts of the plant. Leaves have the highest andrographolide content (2–3%) in comparison with the other plant parts. Adventitious root culture of leaf explants of A. paniculata was studied using different strength MS medium supplemented by different concentrations of auxins and a combination of NAA + kinetin for growth and andrographolide production. Among the different auxin treatments in adventitious root culture, only NAA was able to induce adventitious roots. Adventitious roots grown in modified strength MS medium showed the highest root growth (26.7±1.52), as well as the highest amount of andrographolide (133.3±1.5 mg/g DW) as compared with roots grown in half-and full-strength MS medium. Growth kinetics showed maximum biomass production after five weeks of culture in different strength MS liquid medium. The produced andrographolide content was 3.5- 5.5 folds higher than that of the natural plant, depending on the medium strength.

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Feasible Production of Lignans and Neolignans in Root-Derived In Vitro Cultures of Flax (Linum usitatissimum L.)

Plants ◽

10.3390/plants9040409 ◽

2020 ◽

Vol 9 (4) ◽

pp. 409

Author(s):

Sumaira Anjum ◽

Amna Komal ◽

Samantha Drouet ◽

Humera Kausar ◽

Christophe Hano ◽

...

Keyword(s):

Callus Culture ◽

Adventitious Root ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Root Culture ◽

In Vitro Cultures ◽

Total Phenolic ◽

Adventitious Root Culture

Flax lignans and neolignans impart health benefits, particularly in treating different types of cancers, due to their strong phytoestrogenic and antioxidant properties. The present study enhances the comprehension on the biosynthesis of antioxidant lignans and neolignans in root-derived in vitro cultures of flax (both callus and adventitious root). The results presented here clearly showed that the adventitious root culture efficiently produced a higher amount of lignans (at day 40) and neolignans (at day 30) than callus culture of flax. High performance liquid chromatography (HPLC) analysis revealed that the accumulations of secoisolariciresinol diglucoside (SDG, 5.5 mg g−1 DW (dry weight)) and dehydrodiconiferyl alcohol glucoside (DCG, 21.6 mg/g DW) were 2-fold higher, while guaiacylglycerol-β-coniferyl alcohol ether glucoside (GGCG, 4.9 mg/g DW) and lariciresinol glucoside (LDG, 11.9 mg/g DW) contents were 1.5-fold higher in adventitious root culture than in callus culture. Furthermore, the highest level of total phenolic production (119.01 mg/L), with an antioxidant free radical scavenging activity of 91.01%, was found in adventitious root culture at day 40, while the maximum level of total flavonoid production (45.51 mg/L) was observed in callus culture at day 30 of growth dynamics. These results suggest that adventitious root culture can be a good candidate for scaling up to industrial level to commercially produce these pharmacologically and nutritionally valuable metabolites.

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Establishment of In vitro Adventitious Root Cultures and Analysis of Flavonoids in Rumex crispus

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v25i1.24126 ◽

2015 ◽

Vol 25 (1) ◽

pp. 63-70 ◽

Cited By ~ 1

Author(s):

Majid Mahdieh ◽

Mitra Noori ◽

Simin Hoseinkhani

Keyword(s):

Adventitious Root ◽

Adventitious Roots ◽

Culture Media ◽

Leaf Explants ◽

Root Culture ◽

Root Extract ◽

Rumex Crispus ◽

Adventitious Root Culture ◽

Flavonoid Composition

Adventitious root culture of leaf explants of R. crispus was established using different MS supplemented with different concentrations of auxins and a combination of NAA and Kn for growth and flavonoids production. Among the different auxins, NAA was more effective than IAA to induce adventitious roots. Adventitious roots grown on MS containing 5 ?M NAA and 0.5 ?M Kn showed the highest root growth, as well as the highest amount of total flavonoids (= 6) as compared with roots grown in other media. Chromatographic purification of the root extract showed that flavonoid composition also was influenced by hormone combinations in the culture media. The addition of Kn to the medium reduced or suppressed myricetin (M) and naringenin (N) production. Quercetin (Q) was not found in media containing Kn alone similar to the control medium. Isorhamnetin (I), kaempferol (K) and rutin (R) were produced in the roots on media supplemented with all hormone combinations, but were absent in 0.1 ?M Kn supplemented media similar to the control roots.Plant Tissue Cult. & Biotech. 25(1): 63-70, 2015 (June)

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