scholarly journals A taxonomic revision of the myrmecophilous species of the rattan genus Korthalsia (Arecaceae)

Kew Bulletin ◽  
2019 ◽  
Vol 74 (4) ◽  
Author(s):  
Salwa Shahimi ◽  
Maria Conejero ◽  
Christina J. Prychid ◽  
Paula J. Rudall ◽  
Julie A. Hawkins ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Taxonomic Revision ◽  
Morphological Characterisation ◽  
Microscopic Images ◽  
Distribution Maps ◽  
Taxonomic Treatment ◽  
Future Work ◽  
Scanning Electron

SummaryThe rattan genus Korthalsia Blume (Arecaceae: Calamoideae: Calameae) is widespread in the Malesian region. Among the 28 accepted species are 10 species that form intimate associations with ants. The ants inhabit the conspicuous ocreas that are produced by these species, using them as domatia to care for their young and aphids. As a foundation for future work, we present here a taxonomic treatment of the myrmecophilous Korthalsia species, based on extensive research pursued both in the herbarium and the field. In addition, we conduct detailed morphological characterisation of the structure and development of ocrea using light and scanning electron microscopy. Descriptions, illustrations, keys and distribution maps are presented for all 10 species, along with microscopic images of ocrea morphology and development for selected species.

Taxonomic revision of Riella subgenus Trabutiella (Riellaceae, Sphaerocarpales)

Phytotaxa ◽  
2014 ◽  
Vol 159 (3) ◽  
pp. 131 ◽  
Author(s):  
José Gabriel Segarra-Moragues ◽  
Felisa Puche ◽  
Marko Sabovljevic ◽  
Marta Infante ◽  
Patxi Heras
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Taxonomic Revision ◽  
Distribution Maps ◽  
Ecological Features ◽  
Vegetative Traits ◽  
The Subject ◽  
A New Species ◽  
Scanning Electron

The genus Riella (Riellaceae, Sphaerocarpales) includes some 24 species of thalloid aquatic liverworts with unique morphological and ecological features among hepatics. These include the development of sporophytes enclosed within involucres and growing submerged in seasonal fresh or brackish water ponds. Riella subgenus Trabutiella includes species with winged involucres. Seven taxa have been described, however, four to five taxa have been recognized at a time depending on authors. In this study we have conducted a morphological revision of the species of this subgenus, based on 59 traits measured under light and Scanning Electron Microscopy analyses and that were the subject of statistical analyses. Taxonomically most informative traits were derived from female involucres and spores, whereas quantitative vegetative traits were of little relevance to taxonomy. Lectotypes are designated for subgenus Trabutiella and section Plicatae and for R. cossoniana. Our analyses support the recognition of six species, and support R. paulsenii as a synonym of R. cossoniana. A new species, R. mediterranea is described, and R. cossoniana var. echinata is raised to species rank. An identification key, detailed descriptions of the six accepted taxa, updated distribution maps, and full illustrations of four of the species, including the first for R. echinata are provided.

scholarly journals Pollen morphology of Ukrainian species of the genus Pedicularis L. (Orobanchaceae Vent.)

2012 ◽  
Vol 24 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Olena M. Peregrym ◽  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Taxonomic Revision ◽  
Palynological Data ◽  
First Time ◽  
Scanning Electron

Abstract Pollen grains of 10 species of Pedicularis occurring in Ukraine were investigated using light and scanning electron microscopy. Palynological data on 6 species are reported for the first time. General pollen morphology of the genus Pedicularis is presented. Three types of apertures (2-syncolpate, 3-colpate, and 3-syncolpate) and seven subtypes of sculpture (3-syncolpate pilate, 3-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate, 2-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate-tubeculate-perforate, 2-syncolpate microfoveolate, and 2-syncolpate microscabrate-perforate) are indentified. The subgenus Pedicularis is heterogeneous in its types of apertures and sculpture of the surface, which indicates the need of further taxonomic revision of the group.

Taxonomic revision of Brazilian Tethya (Porifera: Hadromerida) with description of four new species

2011 ◽  
Vol 91 (7) ◽  
pp. 1511-1528 ◽  
Author(s):  
Suzi M. Ribeiro ◽  
Guilherme Muricy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Taxonomic Revision ◽  
The Other ◽  
Scanning Electron

The taxonomy of the genus Tethya from Brazil is revised. Five species are described, four of which are new to science: Tethya maza, T. beatrizae sp. nov., T. nicoleae sp. nov., T. parvula sp. nov., and T. solangeae sp. nov. Five earlier records of Tethya from Brazil (T. aurantium, T. diploderma, T. maza, T. japonica and T. seychellensis) are re-evaluated and only T. maza is confirmed. The other previous records of Tethya from Brazil, viz., Tethya brasiliana, T. cyanae, T. ignis and T. rubra, have been recently described in detail using scanning electron microscopy and are also considered valid. An amendment to the nomenclature of micrasters is made, recognizing at least two different morphologies of each type of micraster (strongylasters types 1 and 2, tylasters types 1 and 2, and oxyasters types 1, 2 and 3). A neotype was designated for T. maza. With four new species described here and four previous records considered invalid the known diversity of Tethya in Brazil still remains nine species. However, its rate of endemism has increased from 44% to 89%.

scholarly journals Taxonomic value of the leaf blade epidermal structure at the sectional level of Altai fescue (Festuca L.)

2020 ◽  
Vol 19 (2) ◽  
pp. 117-122
Author(s):  
E. A. Kriuchkova ◽  
M. V. Olonova ◽  
E. Z. Baiakhmetov ◽  
P. D. Gudkova
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Factor Analysis ◽  
Leaf Blade ◽  
Mixed Data ◽  
Abaxial Side ◽  
Epidermal Structure ◽  
Taxonomic Treatment ◽  
Scanning Electron

Here we present results of our study on the leaf blades epidermis using scanning electron microscopy for 16species of Festuca from Altai. The factor analysis of mixed data revealed markedly differentiated groups according to theirsectional devision. This result supports the previous phylogenetic findings in the genus, except the section Aulaxyper. Thestudy also demonstrates the importance of the abaxial side of leaves in taxonomic treatment of the species, specifically theshape of long cells and silicon bodies, as well as the location of the latter.

scholarly journals SARS-CoV-2: Enhancement and Segmentation of High-ResolutionMicroscopy Images. Part I

2020 ◽  
Author(s):  
Roberto Rodriguez ◽  
Brian A. Mondeja ◽  
Odalys Valdes ◽  
Sonia Resik ◽  
Ananayla Vizcaino ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Eating Habits ◽  
World Population ◽  
The Novel ◽  
Rt Pcr ◽  
Unhealthy Lifestyle ◽  
Microscopic Images ◽  
Novel Coronavirus ◽  
Scanning Electron

Abstract Possibly, and due to poor eating habits and unhealthy lifestyle, many virus are transmitted to human people. Such is the case, of the novel coronavirus SARS-Cov-2, which has expanded of exponential way, practically, to whole world population. For this reason, the study of real microscopic images of this coronavirus is of great importance. The SARS-Cov-2 images were captured from nasopharyngeal samples of Cuban symptomatic individuals (RT-PCR positives for SARS-CoV-2), and processed via scanning electron microscopy. However, many times these microscopic images present some blurring problems, which are always susceptible to be improved. The aim of this work is to propose new computational methods to carry out enhancement and segmentation of SARS-Cov-2 high-resolution microscopic images. Moreover, due to the importance of the obtained results, this first work will be addressed to the application of the proposed algorithm. The proposed strategy obtained very satisfactory results, and we validated its performance, together with specialist physicians, on a set of 1005 images. A second paper will deeply analyze the theory related to these algorithms

scholarly journals A Concept for Three-Dimensional Particle Metrology Based on Scanning Electron Microscopy and Structure-from-Motion Photogrammetry

2020 ◽  
Vol 125 ◽  
Author(s):  
Vipin N. Tondare
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Structure From Motion ◽  
3D Model ◽  
Three Dimensional ◽  
Sem Images ◽  
Shape Information ◽  
Size And Shape ◽  
Future Work ◽  
Scanning Electron

Scanning electron microscopy (SEM) has been frequently used for size and shape measurements of particles. SEM images offer two-dimensional (2D) information about a particle’s lateral dimensions. Unfortunately, information about the particle’s three-dimensional (3D) size and shape remains unavailable. To resolve this issue, I propose a new concept in SEM: 3D particle metrology obtained by applying structure-from-motion (SfM) algorithms to multiple rotational SEM images of particles deposited onto a cylindrical substrate to generate a 3D model from which size and shape information can be extracted. Particles can have any size that is suitable for SEM imaging. SEM images of the sample can be acquired from 0° to 360° using a rotational-tip SEM substage. Here, I will discuss the concept and, for clarity, illustrate it with aquarium gravel particles that are glued onto a craft roll and imaged optically before generating the 3D model of that handmade craft. Future work will include the experimental SEM realization, as well as further development of the SfM algorithms. In my view, this proposed concept may become an integral part of SEM-based particle metrology.

scholarly journals Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia

Lankesteriana ◽  
2021 ◽  
Author(s):  
Edward E. Besi ◽  
Lam S. Jia ◽  
Muskhazli Mustafa ◽  
Christina S. Y.  Yong ◽  
Rusea Go
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Glandular Trichomes ◽  
Taxonomic Revision ◽  
Peninsular Malaysia ◽  
Epicuticular Waxes ◽  
Monophyletic Clade ◽  
Surface Micromorphology ◽  
Floral Micromorphology ◽  
Scanning Electron

Abstract. The floral micromorphology of critically endangered Paphiopedilum Pfitzer [P. barbatum (Lindl.) Pfitzer, P. callosum var. sublaeve (Rchb.f.) P.J.Cribb and P. niveum (Rchb.f.) Stein] were analyzed concerning either infrageneric taxonomy or physioecological demands. The first two species are phylogenetically close and superficially identical but occur with distinct phytogeographical distributions in the region. The third species is a phylogenetically distant congener that inhabits limestone areas in the northern part of Peninsular Malaysia. Using scanning electron microscopy (SEM), we investigated the surface of the dorsal sepal, synsepal, lateral petals, pouch or labellum, and staminode. Amongst the investigated features were epicuticular waxes, epicuticular ornamentation, trichome distribution and type, pustular glands, and papillae. Our study supports the distinction of P. barbatum from P. callosum var. sublaeve, which belong to subgenus Paphiopedilum, and from P. niveum, a species belonging to subgenus Brachypetalum, a separated monophyletic clade. Comparatively, P. barbatum has Type III non-glandular trichomes on the margin of its lateral petals, which are absent in P. callosum var. sublaeve. Paphiopedilum callosum var. sublaeve and P. niveum are distinguishable from P. barbatum by a confined distribution of papillae. The epicuticular ornamentation and distribution of trichomes on staminode discriminate between P. barbatum and P. callosum var. sublaeve and differentiates them from P. niveum. Compared to P. barbatum and P. niveum, stomata in P. callosum var. sublaeve were superficial with prominently raised guard cells. From the physioecological view, the absence of glandular trichomes, and the low occurrence of papillae and stomata on the floral parts explain the unscented flowers of P. barbatum and P. callosum var. sublaeve. A combination of the features examined is taxonomically valuable for delimitation of the species at the infrageneric level, although the diagnostic characters are far inadequate for a generic taxonomic revision. A study with a more extensive sampling from the three subgenera of Paphiopedilum, including subgenus Parvisepalum, is anticipated to elucidate the level of variation of the analyzed microcharacters. Key Words: physioecological importance, Peninsular Malaysia, subgenus Brachypetalum, subgenus Paphiopedilum, scanning electron microscopy, taxonomic delimitation

A new Isoetes hybrid (Isoetes echinospora × riparia) for Canada

1989 ◽  
Vol 67 (10) ◽  
pp. 2995-3002 ◽  
Author(s):  
D. M. Britton ◽  
D. F. Brunton
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Interspecific Hybrid ◽  
Hybrid Vigour ◽  
Triploid Hybrid ◽  
Distribution Maps ◽  
Scanning Electron

A new interspecific hybrid in Isoetes, Isoetes echinospora Dur. × Isoetes riparia Engelm., is delineated by means of cytology and scanning electron microscopy of spores from the Ottawa–Hull district of Canada. The hybrid should be known as I. × dodgei A. A. Eaton pro sp. This triploid hybrid (2n = 33) exhibits polymorphic megaspores and hybrid vigour. The megaspores of some of the hybrids are extremely spiny, with many fused and irregular spines resulting in convoluted and distorted surfaces. Distribution maps for three species, I. echinospora, I. riparia, and I. macrospora Dur., as well as one hybrid, I. × dodgei, are presented for the Ottawa district.

scholarly journals Taxonomic revision of Galeocharax (Characiformes: Characidae: Characinae)

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
Victor Giovannetti ◽  
Mônica Toledo-Piza ◽  
Naércio A. Menezes
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Taxonomic Revision ◽  
South American ◽  
Morphometric Data ◽  
Key To Species ◽  
Madre De Dios ◽  
Rio Negro ◽  
Scanning Electron ◽  
Rio Madeira

ABSTRACT The taxonomy of Galeocharax, a genus of freshwater fishes distributed in most South American cis-Andean river systems, except the rio Negro, rio São Francisco and rio Xingu basins and the eastern drainages of Brazil, is herein revised. A total of 1229 specimens were examined from which 680 had meristic and morphometric data taken. Osteological and morphological features were also examined through radiographs, scanning electron microscopy and in cleared and stained specimens. Three of the four species previously considered as valid are herein recognized: Galeocharax humeralis from rio Paraguay and lower rio Paraná basins; Galeocharax goeldii, from rio Madeira basin, with records of geographical distribution expanded to the río Madre de Dios, río Beni, rio Mamoré and rio Guaporé basins and Galeocharax gulo, which is widespread throughout rio Amazonas, río Orinoco, rio Tocantins, and upper rio Paraná basins. Galeocharax knerii is herein considered a junior synonym of Galeocharax gulo. A key to species of Galeocharax is presented.

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