Correction to: Assessing the Contribution of Sli to Self-Compatibility in North American Diploid Potato Germplasm Using KASP™ Markers

Ascorbic acid (vitamin C) is an essential nutrient in the human diet and potatoes are a valuable source. As a first step in breeding for potatoes (Solanum tuberosum L.) with higher levels of ascorbic acid, 75 clones from 12 North American potato-breeding programs were evaluated for concentration, and 10 of those for stability of expression. Trials were grown in Idaho, Oregon, and Washington in 1999 and 2000, tubers sampled, and ascorbic acid quantified. There were significant differences among clones and clone by environment interaction was also significant. Concentration of ascorbic acid of the clones was continuously distributed over a range of 11.5 to 29.8 mg/100 g. A subgroup of 10 clones was analyzed using an additive main effects and multiplicative interaction (AMMI) model, to diagnose interaction patterns and measure clone stability. The first two principal component axes accounted for over 80% of the variability. Bi-plot analysis showed `Ranger Russet' to be highly unstable across the environments tested. A plot of Tai's stability statistics found six of the 10 clones to be stable for ascorbic acid expression. Appropriate evaluation methods for ascorbic acid concentration must involve multi-year testing.

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ASCORBIC ACID CONCENTRATION AND STABILITY IN NORTH AMERICAN POTATO GERMPLASM

Acta Horticulturae ◽

10.17660/actahortic.2003.619.10 ◽

2003 ◽

pp. 87-93 ◽

Cited By ~ 12

Author(s):

S.L. Love ◽

T. Salaiz ◽

B. Shafii ◽

W.J. Price ◽

A.R. Mosley ◽

...

Keyword(s):

Ascorbic Acid ◽

Acid Concentration ◽

North American ◽

Ascorbic Acid Concentration ◽

Potato Germplasm

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Toward the development of highly homozygous diploid potato lines using the self-compatibility controlling Sli gene

Genome ◽

10.1139/g05-066 ◽

2005 ◽

Vol 48 (6) ◽

pp. 977-984 ◽

Cited By ~ 33

Author(s):

Chalermpol Phumichai ◽

Motoyuki Mori ◽

Akira Kobayashi ◽

Osamu Kamijima ◽

Kazuyoshi Hosaka

Keyword(s):

Genetic Map ◽

Average Rate ◽

The Self ◽

Aflp Markers ◽

Diploid Potato ◽

Average Heterozygosity ◽

Diploid Clone ◽

Self Compatibility ◽

Homozygous Diploid ◽

Sli Gene

Cultivated diploid potatoes (2n = 2x = 24) are self-incompatible, but can be altered to become self-compatible using the Sli gene. Previously, a diploid clone 97H32–6 was selfed up to S3 using the Sli gene. To explore the usefulness of the Sli gene for the production of highly homozygous diploid potatoes, 2 S4 families from the above 97H32-6 derived S3 lines (inbred series A) and 3 S5 families by continuous selfings from a different F1 (= S0) plant (inbred series B) were developed. The level of heterozygosity and the location of heterozygous loci on the genetic map were investigated using RFLP and AFLP markers. The average heterozygosity levels of the originally heterozygous loci decreased from 100% in S0 to 10.7% in S4 and 8.6% in S5 (inbred series A and B, respectively). The average rate of reduction in heterozygosity per generation (38.4% and 38.5% for inbred series A and B, respectively) was lower than the theoretically expected rate (50%). However, none of the loci or chromosome sections was exclusively heterozygous in the advanced self-progeny. Thus, highly homozygous and seed-propagated diploid potatoes could be obtained by repeated selfing using the Sli gene.Key words: Selfing, heterozygosity, diploid potato, Sli gene, Solanum.

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Developing self-compatible diploid potato germplasm through recurrent selection

Euphytica ◽

10.1007/s10681-021-02785-0 ◽

2021 ◽

Vol 217 (3) ◽

Author(s):

Maher Alsahlany ◽

Felix Enciso-Rodriguez ◽

Marco Lopez-Cruz ◽

Joseph Coombs ◽

David S. Douches

Keyword(s):

Recurrent Selection ◽

Diploid Potato ◽

Potato Germplasm

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Single nucleotide polymorphism discovery in elite north american potato germplasm

BMC Genomics ◽

10.1186/1471-2164-12-302 ◽

2011 ◽

Vol 12 (1) ◽

Cited By ~ 131

Author(s):

John P Hamilton ◽

Candice N Hansey ◽

Brett R Whitty ◽

Kevin Stoffel ◽

Alicia N Massa ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

North American ◽

Single Nucleotide Polymorphism Discovery ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Potato Germplasm ◽

Polymorphism Discovery

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Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato

Nature Communications ◽

10.1038/s41467-021-24267-6 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Ernst-Jan Eggers ◽

Ate van der Burgt ◽

Sjaak A. W. van Heusden ◽

Michiel E. de Vries ◽

Richard G. F. Visser ◽

...

Keyword(s):

Genetic Gain ◽

F1 Hybrid ◽

Precision Breeding ◽

Potato Germplasm ◽

Pollen Rejection ◽

Self Compatibility ◽

Screen Approach ◽

F Box Protein ◽

And Function ◽

S Locus Inhibitor

AbstractGenetic gain in potato is hampered by the heterozygous tetraploid genome of cultivated potato. Converting potato into a diploid inbred-line based F1-hybrid crop provides a promising route towards increased genetic gain. The introduction of a dominant S-locus inhibitor (Sli) gene into diploid potato germplasm allows efficient generation of self-fertilized seeds and thus the development of potato inbred lines. Little is known about the structure and function of the Sli locus. Here we describe the mapping of Sli to a 12.6 kb interval on chromosome 12 using a recombinant screen approach. One of two candidate genes present in this interval shows a unique sequence that is exclusively present in self-compatible lines. We describe an expression vector that converts self-incompatible genotypes into self-compatible and a CRISPR-Cas9 vector that converts SC genotypes into SI. The Sli gene encodes an F-box protein that is specifically expressed in pollen from self-compatible plants. A 533 bp insertion in the promotor of that gene leads to a gain of function mutation, which overcomes self-pollen rejection.

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