Re-examination of metabolic fluxes in Escherichia coli during anaerobic fermentation of glucose using 13C labeling experiments and 2-dimensional nuclear magnetic resonance (NMR) spectroscopy

2011 ◽  
Vol 16 (3) ◽  
pp. 419-437 ◽  
Author(s):  
Madhuresh K. Choudhary ◽  
Jong Moon Yoon ◽  
Ramon Gonzalez ◽  
Jacqueline V. Shanks
Keyword(s):  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Anaerobic Fermentation ◽  
13C Labeling ◽  
Metabolic Fluxes ◽  
Nuclear Magnetic

scholarly journals A Revised Structure for the Glycolipid Terminus of Escherichia coli K5 Heparosan Capsular Polysaccharide

Biomolecules ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1516
Author(s):  
Lufeng Yan ◽  
Li Fu ◽  
Ke Xia ◽  
Shiguo Chen ◽  
Fuming Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Capsular Polysaccharide ◽  
Enzymatic Digestion ◽  
Nuclear Magnetic

The structure of heparosan capsular polysaccharide (CPS) has been determined using enzymatic digestion with nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Previous errors in the assignment of the glycolipid acceptor structure, from which heparosan is extended, have been corrected. The structure of heparosan CPS is GlcNAc α-1,[4GlcA β-1,4GlcNAc α-1,]n4GlcA β-1,[4Kdo β-2,7Kdo β-2,]0 or 14Kdo β-2,7Kdo β-2,4Kdo β-2,7Kdo β-2,4Kdo β-2,7Kdo β-2,4Kdo β-PG-I (C16:0 or C18:0) (where n is ~250 for a CPS of 100 kDa).

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