Comparison of three Pediococcus strains for lactic acid production from glucose in the presence of inhibitors generated by acid hydrolysis of lignocellulosic biomass

2013 ◽  
Vol 18 (6) ◽  
pp. 1192-1200 ◽  
Author(s):  
Yang Liu ◽  
Somasundar Ashok ◽  
Eunhee Seol ◽  
Jie Bao ◽  
Sunghoon Park
2010 ◽  
Vol 101 (3) ◽  
pp. 1036-1043 ◽  
Author(s):  
Pattana Laopaiboon ◽  
Arthit Thani ◽  
Vichean Leelavatcharamas ◽  
Lakkana Laopaiboon

2021 ◽  
pp. 125880
Author(s):  
Kevin Tian Xiang Tong ◽  
Inn Shi Tan ◽  
Henry Chee Yew Foo ◽  
Adrian Chiong Yuh Tiong ◽  
Man Kee Lam ◽  
...  

Bionatura ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 1803-1811
Author(s):  
Shirley Inguillay ◽  
Felipe Jadán ◽  
Pedro Maldonado-Alvarado

Ecuador is an agricultural country, which has an actual production of starch obtained from cassava. Tuber processing residues do not have an economic impact; for example, the cassava bagasse, only used for plant fertilization and animal feeding. This project aimed to study the influence of the fermentation variables (pH and agitation), on the lactic acid production. Enzymatic hydrolysis of cassava bagasse starch for the varieties INIAP 650 and INIAP 651 was performed using α-amylase and glucoamylase. Then, glucose was fermented by Lactobacillus leichmannii ATCC 7830 strains, varying conditions such as agitation (150 rpm and absence) and pH (4.5, 5.0, and 5.5). Finally, the determination of lactic acid was performed by potentiometric and FTIR analysis. Conversions of cassava bagasse to reduced sugars were 71.66 and 85.05 % for INIAP 650 and INIAP 651 varieties. The best lactic acid concentrations were 27.62 and 33.48 g/L, obtained at pH 5.5 and agitation, for INIAP 650 and INIAP 651 varieties. Qualitative analysis conducted by FTIR spectrophotometry confirmed the presence of lactic acid in the reacted products. Lactic acid production from cassava bagasse starch could contribute to the Manabí and Esmeraldas provinces of Ecuador's economic development.


BioResources ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 1379-1389
Author(s):  
Yingnan Cao ◽  
Juan Wang ◽  
Qunhui Wang ◽  
Jianguo Liu ◽  
Tingxi Liu ◽  
...  

Efficient pretreatment and enzymatic hydrolysis is critical to achieve effective utilization of lignocellulosic biomass. In this study, the cellulase composition for lignocellulosic biomass hydrolysis was strategically optimized to improve the efficiency of vinasse saccharification and thus enhance L-lactic acid production. The results showed that the supplementation of β-glycosidase (BG) increased sugar production, and the glucose concentration exceeded cellobiose concentration after 48 h of hydrolysis. These results suggested that the addition of BG aided the hydrolysis of cellobiose and reduced the inhibitory effects caused by sugar accumulation. After 72 h to 96 h of hydrolysis, the BG supplementation improved cellobiose and glucose production by 25.7% and 27.4%, respectively. The effect of BG supplementation on L-lactic acid production during the fermentation of microwave-alkali pretreated vinasse was also investigated. Here, the L-lactic acid production from simultaneous saccharification and fermentation (SSF) with the addition of BG was 20.8% higher than that without BG addition, and was also 37.0% higher than production from separate hydrolysis and fermentation with BG addition. These results indicated the utilization efficiency of lignocellulosic biomass for L-lactic acid production could be enhanced by supplementation of BG in SSF.


2021 ◽  
Author(s):  
Kedong Ma ◽  
Yubo Cui ◽  
Ke Zhao ◽  
Yuxuang Yang ◽  
Yidan Wang ◽  
...  

Abstract Background: D-lactic acid played an important role in the establishment of PLA as a substitute for petrochemical plastics. But, so far, the D-lactic acid production was limited in only pilot scale, which was definitely unable to meet the fast growing market demand. To achieve industrial scale D-lactic acid production, the cost-associated problems such as high-cost feedstock, expensive nutrient sources and fermentation technology need to be resolved to establish an economical fermentation process.Results: In the present study, the combined effect of B vitamin supplementation and membrane integrated continuous fermentation on D-lactic acid production from agricultural lignocellulosic biomass by Lactobacillus delbrueckii was investigated. The results indicated the specific addition of vitamins B1, B2, B3 and B5 could reduce the yeast extract (YE) addition from 10 g/l to 3 g/l without obvious influence on fermentation efficiency. By employing cell recycling system in 350 h continuous fermentation with B vitamin supplementation, YE addition was further reduced to 0.5 g/l, which resulted in nutrient source cost reduction of 86%. A maximum D-lactate productivity of 18.56 g/l/h and optical purity of 99.5% were achieved and higher than most recent reports. Conclusion: These findings suggested the novel fermentation strategy proposed could effectively reduce the production cost and improve fermentation efficiency, thus exhibiting great potential in promoting industrial scale D-lactic acid production from lignocellulosic biomass.


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