Utilization for lactic acid production with a new acid hydrolysis of ram horn waste

Ecuador is an agricultural country, which has an actual production of starch obtained from cassava. Tuber processing residues do not have an economic impact; for example, the cassava bagasse, only used for plant fertilization and animal feeding. This project aimed to study the influence of the fermentation variables (pH and agitation), on the lactic acid production. Enzymatic hydrolysis of cassava bagasse starch for the varieties INIAP 650 and INIAP 651 was performed using α-amylase and glucoamylase. Then, glucose was fermented by Lactobacillus leichmannii ATCC 7830 strains, varying conditions such as agitation (150 rpm and absence) and pH (4.5, 5.0, and 5.5). Finally, the determination of lactic acid was performed by potentiometric and FTIR analysis. Conversions of cassava bagasse to reduced sugars were 71.66 and 85.05 % for INIAP 650 and INIAP 651 varieties. The best lactic acid concentrations were 27.62 and 33.48 g/L, obtained at pH 5.5 and agitation, for INIAP 650 and INIAP 651 varieties. Qualitative analysis conducted by FTIR spectrophotometry confirmed the presence of lactic acid in the reacted products. Lactic acid production from cassava bagasse starch could contribute to the Manabí and Esmeraldas provinces of Ecuador's economic development.

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Microwave Assisted Enzymatic Hydrolysis of Larch for L-Lactic Acid Production

2010 4th International Conference on Bioinformatics and Biomedical Engineering ◽

10.1109/icbbe.2010.5516665 ◽

2010 ◽

Author(s):

Xue-xin Yang ◽

Gui-zhen Fang

Keyword(s):

Lactic Acid ◽

Enzymatic Hydrolysis ◽

Acid Production ◽

Lactic Acid Production ◽

Microwave Assisted ◽

Hydrolysis Of

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CO-PRODUCTION OF LACTIC ACID AND ETHANOL USING RHIZOPUS SP. FROM HYDROLYZED INEDIBLE CASSAVA STARCH AND LEAVES

IIUM Engineering Journal ◽

10.31436/iiumej.v17i2.610 ◽

2016 ◽

Vol 17 (2) ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Azlin Suhaida Azmi ◽

Nurlisa Yusuf ◽

Dzun Noraini Jimat ◽

Noor Illi Mohamad Puad

Keyword(s):

Lactic Acid ◽

Acid Production ◽

Lactic Acid Production ◽

Cassava Starch ◽

Optimum Conditions ◽

Glucose Yield ◽

Cassava Leaves ◽

Ethanol Yields ◽

Rhizopus Sp ◽

Hydrolysis Of

Production of lactic acid and ethanol from inedible cassava starch and leaves was investigated. Prior to fermentation, hydrolysis of the starch and leaves was conducted. Hydrolysis was optimized by manipulating at three levels each four particular factors, namely acid concentration, starch concentration, temperature, and reaction time. Maximum glucose yield of 0.96 g/g was obtained when 2.5% (w/v) of cassava leaves with 2.5% (w/v) of starch was hydrolyzed using 0.20 M of nitric acid at 160oC of temperature for 10 min. The potential of hydrolyzed cassava starch and leaves was then investigated for lactic acid production using fungal Rhizopus sp. The fermentation process was then conducted in shake flask by varying four factors at three levels each. Maximum lactic acid and ethanol yields of 0.95 g/g and 0.52 g/g, respectively, were achieved at different optimum conditions. Â Lactic acid production was found to be linked to a decrease in ethanol production. Design Expert v6.0.8 was used to aid in the design of the experiment using Taguchiâ€™s methodology.

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Effect of Pretreatment Methods on L-Lactic Acid Production from Vinasse Fermentation

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.113-116.1302 ◽

2010 ◽

Vol 113-116 ◽

pp. 1302-1305 ◽

Cited By ~ 5

Author(s):

Jian Guo Liu ◽

Qun Hui Wang ◽

Hong Zhi Ma ◽

Shuang Wang

Keyword(s):

Lactic Acid ◽

Acid Production ◽

Lactic Acid Production ◽

Value Added ◽

Raw Material ◽

Alkali Concentration ◽

Utilization Rate ◽

Fermentation Time ◽

Optimum Parameters ◽

Hydrolysis Of

To achieve high value-added utilization, vinasse was chosen as raw material for l-lactic acid production. Alkali methods, microwave were used to treat vinasse and the optimum parameters during the process were investigated. The results showed that the best pretreatment way was the combination of alkali concentration of 8% and microwave power 700W, corresponding maximum lactic acid concentration was 17.5g/L, higher than those of the independent method utilized, the fermentation time could be one day earlier than other methods. The conversion rate of preprocessed vinasse to L-lactic acid and utilization rate of cellulose and hemi-cellulose could reach 23.8% and 71%, respectively. The optimized pretreatment could effectively realize hydrolysis of vinasse and make it an ideal sources for further utilization.

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