Enhanced production of cellobiase by marine bacterium Cellulophaga lytica LBH-14 from rice bran under optimized conditions involved in dissolved oxygen

In the present paper, parametric optimization studies were performed to determine the most influential range of process parameters for maximum ethanol production from Pichia stipitis NCIM3499 by one-at-a-time method under submerged fermentation conditions. Various parameters, such as agitation, pH of medium, temperature and different carbon sources, etc., were taken into consideration. The optimum values of these process parameters were as follows: agitation (200 rpm), pH (5.5), temperature (30°C), and semi-aerobic conditions (150 ml fermentation media in 250 ml Erlenmeyer flask). Among the different carbon substrates tested, glucose and xylose (20±0.21 g/l) showed same ethanol production (8.8±0.35 g/l) with a yield of 0.44±0.11g/g sugar utilized. These process parameters were applied for the ethanol production from de-oiled rice bran (DORB), the left over residue after extraction of oil. It was thermochemically saccharified using sulfuric acid (0.5-5.5% v/v) at 120°C for a fixed residence time of 1h. A maximum of 38.50±0.45 g/l total reducing sugars was obtained at 3.5% v/v H2SO4. This sugar syrup was detoxified by overliming with calcium hydroxide and subsequently fermented with P. stipitis NCIM3499 under the optimized conditions. After 72 h of incubation, DORB hydrolysate (33.50±0.44g/l total reducing sugars) showed maximum ethanol production (12.47±0.26g/l) with the yield 0.42±0.021g/g and fermentation efficiency of 81.74±0.55%. To the best of our knowledge, this is the first report on ethanol production from de-oiled rice bran using P. stipitis NCIM3499.

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Enhanced production of carboxymethylcellulase of a marine microorganism, Bacillus subtilis subsp. subtilis A-53 in a pilot-scaled bioreactor by a recombinant Escherichia coli JM109/A-53 from rice bran

Molecular Biology Reports ◽

10.1007/s11033-012-2435-9 ◽

2013 ◽

Vol 40 (5) ◽

pp. 3609-3621 ◽

Cited By ~ 14

Author(s):

Eun-Jung Lee ◽

Bo-Hwa Lee ◽

Bo-Kyung Kim ◽

Jin-Woo Lee

Keyword(s):

Escherichia Coli ◽

Bacillus Subtilis ◽

Rice Bran ◽

Recombinant Escherichia Coli ◽

Marine Microorganism ◽

Enhanced Production ◽

Escherichia Coli Jm109 ◽

Subtilis Subsp

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Optimisation of rice bran hydolysation using xylanase for xylooligosacchride production

TAP CHI SINH HOC ◽

10.15625/0866-7160/v40n2.9931 ◽

2018 ◽

Vol 40 (2) ◽

pp. 259-265

Author(s):

Nguyen Thi Mai Phuong ◽

Nguyen Hoa Anh ◽

Pham Thi Ngoc ◽

Quach Thi Lien ◽

Nguyen Thi Bich

Keyword(s):

Experimental Data ◽

Food Safety ◽

Rice Bran ◽

High Purity ◽

Appropriate Technology ◽

Quadratic Model ◽

Technological Properties ◽

Soluble Fiber ◽

Beneficial Bacteria ◽

Optimized Conditions

Rice bran is a subsidised product of rice processing. It is rich in carbohydrate, especially xylan therefore, has being used for production of soluble fiber oligosaccharide including xylooligosaccharides (XOS). XOS is an oligomer of 2-7 xylose residues and has been proven to be fermented by beneficial bacteria Bifidobacterium and Lactobacillus in colon. The market for XOS is increasing rapidly due to its advantages in biological and technological properties, compared to other common oligosaccharides, such as fructooligosaccharide or galactooligosaccharide. XOS can be produced from rice bran using either chemical or enzymatic hydrolysation technologies. The hydrolyzation using β-1,4-xylanase is commonly used to produce XOS from rice bran. However, an appropriate technology for XOS production from rice bran with high purity and food safety in Vietnam is not available yet. This paper presents new data on optimisation of rice bran hydrolyzation by xylanase to produce XOS using the quadratic model of Box-Behnken. The theoretically optimized conditions for the hydrolyzation are 0.25% enzyme at pH 5.5, temperature of 50°C for 18 hours. Experimental data confirmed the selected condition. The XOS product contains mainly xylobiose, xylotriose and xylotetraose.

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