scholarly journals Optimisation of rice bran hydolysation using xylanase for xylooligosacchride production

2018 ◽  
Vol 40 (2) ◽  
pp. 259-265
Author(s):  
Nguyen Thi Mai Phuong ◽  
Nguyen Hoa Anh ◽  
Pham Thi Ngoc ◽  
Quach Thi Lien ◽  
Nguyen Thi Bich

Rice bran is a subsidised product of rice processing. It is rich in carbohydrate, especially xylan therefore, has being used for production of soluble fiber oligosaccharide including xylooligosaccharides (XOS). XOS is an oligomer of 2-7 xylose residues and has been proven to be fermented by beneficial bacteria Bifidobacterium and Lactobacillus in colon. The market for XOS is increasing rapidly due to its advantages in biological and technological properties, compared to other common oligosaccharides, such as fructooligosaccharide or galactooligosaccharide. XOS can be produced from rice bran using either chemical or enzymatic hydrolysation technologies. The hydrolyzation using β-1,4-xylanase is commonly used to produce XOS from rice bran. However, an appropriate technology for XOS production from rice bran with high purity and food safety in Vietnam is not available yet. This paper presents new data on optimisation of rice bran hydrolyzation by xylanase to produce XOS using the quadratic model of Box-Behnken. The theoretically optimized conditions for the hydrolyzation are 0.25% enzyme at pH 5.5, temperature of 50°C for 18 hours. Experimental data confirmed the selected condition. The XOS product contains mainly xylobiose, xylotriose and xylotetraose.

2021 ◽  
Vol 45 (4) ◽  
pp. 273-280
Author(s):  
Raju Kalakuntala ◽  
Srinath Surnani

The performance of heteropoly acid i.e., Tungstan phosphoric acid for the synthesis of butyl propionate at optimized conditions. Effect on conversion and yield of propionic acids using the Response Surface Methodology (RSM) were evaluated by different process parameters including catalyst loading, alcohol/acid molar ratio. There were no external and internal mass transmission limits. A quadratic model acquired by the variance study (ANOVA) has been shown to view experimental data successfully with the regression (R2 = 0.94 and R2 = 0.942) coefficients approaching to unity. The pseudo homogeneous kinetic model (PH) validated with experimental data to determine kinetic parameters i.e., activation energy (45.97 kJ/mol) and frequent factor (91319 L/mol-min).


2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Kajal Gautam ◽  
Rishi K. Verma ◽  
Suantak Kamsonlian ◽  
Sushil Kumar

AbstractThe present study is aimed to model and optimize the electrocoagulation (EC) process with five important parameters for the decolorization of Reactive Black B (RBB) from simulated wastewater. A multivariate approach, response surface methodology (RSM) together with central composite design (CCD) is used to optimize process parameters such as pH (5–9), electrode gap (0.5–2.5 cm), current density (2.08–10.41 mA/cm2), process time (10–30 min), and initial dye concentration (100–500 mg/l). The predicted percentage decolorization of dye is obtained as 97.21% at optimized conditions: pH (6.8), gapping (1.3 cm), current density (8.32 mA/cm2), time (23 min), and initial dye concentration (200 mg/L), which is very close to experimental percent decolorization (98.41%). The statistical analysis of variance (ANOVA) is performed to evaluate the quadratic model (RSM), and shows good fit of experimental data with coefficient of determination R2 >0.93. An Artificial Neural Network (ANN) is also used to predict the percentage decolorization and gives overall 94.96% which shows performance accuracy between the predicted and actual value of decolorization. The additional considerations of operating cost and current efficiency are also taken care to show the efficacy of EC process with mathematical tool. The sludge characteristics are determined by FE-SEM/EDX.


Inventions ◽  
2021 ◽  
Vol 6 (3) ◽  
pp. 50
Author(s):  
Silvia Lazăr (Mistrianu) ◽  
Oana Emilia Constantin ◽  
Nicoleta Stănciuc ◽  
Iuliana Aprodu ◽  
Constantin Croitoru ◽  
...  

(1) Background: This study is designed to extract the bioactive compounds from beetroot peel for future use in the food industry. (2) Methods: Spectrophotometry techniques analyzed the effect of conventional solvent extraction on betalains and polyphenolic compounds from beetroot peels. Several treatments by varying for factors (ethanol and citric acid concentration, temperature, and time) were applied to the beetroot peel samples. A Central Composite Design (CCD) has been used to investigate the effect of the extraction parameters on the extraction steps and optimize the betalains and total polyphenols extraction from beetroot. A quadratic model was suggested for all the parameters analyzed and used. (3) Results: The maximum and minimum variables investigated in the experimental plan in the coded form are citric acid concentration (0.10–1.5%), ethanol concentration (10–50%), operating temperature (20–60 °C), and extraction time (15–50 min). The experimental design revealed variation in betalain content ranging from 0.29 to 1.44 mg/g DW, and the yield of polyphenolic varied from 1.64 to 2.74 mg/g DW. The optimized conditions for the maximum recovery of betalains and phenols were citric acid concentration 1.5%, ethanol concentration 50%, temperature 52.52 °C, and extraction time 49.9 min. (4) Conclusions: Overall, it can be noted that the extraction process can be improved by adjusting operating variables in order to maximize the model responses.


2021 ◽  
Vol 98 ◽  
pp. 14-18
Author(s):  
Thao Nguyen Thi ◽  
◽  
Nam Pham Ky ◽  
Ngoc Tran Vu Diem

Brass melting slag (20.38 wt.% Zn) was leached in sulfuric acid with concentration of (50 + 80) g/l H2SO4, leaching temperature of (30 + 60) °C for (30 + 120) min. The optimized conditions for 94.16% Zn extraction from brass melting slag were found as 70 g/l H2SO4, room temperature and 90 min. The leaching solution was purified by removal of Fe through Fe(OH)3 precipitation when adding ZnO to adjust pH value of 5. The solution was continuously cemented by Zn metal at 60 °C for 60 min to obtain Cu metal with high purity of 99 wt.% Cu. The purified solution with 37.64 g/l Zn was modified by Na2C03 to have pH value of about 6 and precipitation of ZnC03 (94.14 %).


2014 ◽  
Vol 50 (2) ◽  
pp. 290-297 ◽  
Author(s):  
Chiemela E. Chinma ◽  
Muna Ilowefah ◽  
Balakrishnan Shammugasamy ◽  
Makeri Mohammed ◽  
Kharidah Muhammad

2020 ◽  
Vol 87 (2) ◽  
pp. 259-262
Author(s):  
Monique Colombo ◽  
Svetoslav D. Todorov ◽  
Antonio F. Carvalho ◽  
Luís A. Nero

AbstractIn this research paper we describe the technological properties of beneficial lactic acid bacteria (LAB) obtained from a dairy production chain and the development of a fermented milk produced with Lactobacillus casei MRUV6. Fifteen LAB isolates (Lactobacillus sp., Pediococcus sp. and Weissela sp.) presented acidifying abilities (pH ranges from 0.73 to 2.11), were able to produce diacetyl (except by 5 isolates) and exopolysaccharides, and two were proteolytic. L. casei MRUV6 was selected for producing a fermented milk, stored up to 35 d at 4 and 10°C. Counts on MRS agar with added vancomycin (10 mg/l) and MRS agar with added bile salts (1.5% w/v) ranged from 9.7 to 9.9 log CFU/g, independently of the tested conditions, indicating stability and intestinal resistance of L. casei MRUV6, despite some significant differences (P < 0.05). The study demonstrated the technological potential of a potential probiotic candidate strain, L. casei MRUV6, to be used as a starter culture in the dairy industry.


2018 ◽  
Vol 54 (4) ◽  
pp. 1321-1329 ◽  
Author(s):  
Maria Alexandri ◽  
Anna‐Katrin Neu ◽  
Roland Schneider ◽  
José Pablo López‐Gómez ◽  
Joachim Venus

ENERGYO ◽  
2018 ◽  
Author(s):  
Anuj Kumar Chandel ◽  
Mangamoori Lakshmi Narasu ◽  
Ravinder Rudravaram ◽  
Ravindra Pogaku ◽  
Linga Venkateswar Rao

2002 ◽  
Vol 124 (3) ◽  
pp. 290-296 ◽  
Author(s):  
David H. Lassila ◽  
Mary M. LeBlanc ◽  
Gregory J. Kay

An apparatus has been developed for performing compression deformation experiments on oriented metallic single crystals to provide data for validation of 3-D dislocation dynamics simulations. The experiment is performed under conditions that allow unconstrained motion of the upper and lower compression platen, and thus a relatively uniform state of axial stress is maintained during the deformation. Experiments have been performed on high-purity Mo single crystal and polycrystalline Cu. Various aspects of the experimental procedures and results are presented. Possible usages of the experimental data for the validation of 3-D dislocation dynamics simulations are discussed.


Author(s):  
Anuj Kumar Chandel ◽  
Mangamoori Lakshmi Narasu ◽  
Ravinder Rudravaram ◽  
Ravindra Pogaku ◽  
Linga Venkateswar Rao

In the present paper, parametric optimization studies were performed to determine the most influential range of process parameters for maximum ethanol production from Pichia stipitis NCIM3499 by one-at-a-time method under submerged fermentation conditions. Various parameters, such as agitation, pH of medium, temperature and different carbon sources, etc., were taken into consideration. The optimum values of these process parameters were as follows: agitation (200 rpm), pH (5.5), temperature (30°C), and semi-aerobic conditions (150 ml fermentation media in 250 ml Erlenmeyer flask). Among the different carbon substrates tested, glucose and xylose (20±0.21 g/l) showed same ethanol production (8.8±0.35 g/l) with a yield of 0.44±0.11g/g sugar utilized. These process parameters were applied for the ethanol production from de-oiled rice bran (DORB), the left over residue after extraction of oil. It was thermochemically saccharified using sulfuric acid (0.5-5.5% v/v) at 120°C for a fixed residence time of 1h. A maximum of 38.50±0.45 g/l total reducing sugars was obtained at 3.5% v/v H2SO4. This sugar syrup was detoxified by overliming with calcium hydroxide and subsequently fermented with P. stipitis NCIM3499 under the optimized conditions. After 72 h of incubation, DORB hydrolysate (33.50±0.44g/l total reducing sugars) showed maximum ethanol production (12.47±0.26g/l) with the yield 0.42±0.021g/g and fermentation efficiency of 81.74±0.55%. To the best of our knowledge, this is the first report on ethanol production from de-oiled rice bran using P. stipitis NCIM3499.


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