scholarly journals Morphological changes of cholinergic nerve fibers in the urinary bladder after establishment of artificial somatic-autonomic reflex arc in rats

2007 ◽  
Vol 23 (5) ◽  
pp. 277-281 ◽  
Author(s):  
Han-Zhi Wang ◽  
Shu-Rong Li ◽  
Can Wen ◽  
Chuan-Guo Xiao ◽  
Bing-Yin Su
Keyword(s):  
Urinary Bladder ◽  
Morphological Changes ◽  
Nerve Fibers ◽  
Cholinergic Nerve ◽  
Reflex Arc ◽  
Autonomic Reflex ◽  
Cholinergic Nerve Fibers

scholarly journals Identification of the origin of adrenergic and cholinergic nerve fibers within the superior hypogastric plexus of the human fetus

2013 ◽  
Vol 223 (1) ◽  
pp. 14-21 ◽  
Author(s):  
Mazen Zaitouna ◽  
Bayan Alsaid ◽  
Djibril Diallo ◽  
Gérard Benoit ◽  
Thomas Bessede
Keyword(s):  
Human Fetus ◽  
Nerve Fibers ◽  
Cholinergic Nerve ◽  
Hypogastric Plexus ◽  
Superior Hypogastric Plexus ◽  
Cholinergic Nerve Fibers

Aminergic and cholinergic innervations of the spinal cord blood vessels of cats

1983 ◽  
Vol 58 (6) ◽  
pp. 900-905 ◽  
Author(s):  
Toru Itakura
Keyword(s):  
Spinal Cord ◽  
Cord Blood ◽  
Blood Vessels ◽  
Nerve Fibers ◽  
Anterior Spinal Artery ◽  
Thoracic Spinal Cord ◽  
Cholinergic Nerve ◽  
Ischemic Change ◽  
Thoracic Spinal ◽  
Cholinergic Nerve Fibers

✓ The distribution patterns of aminergic and cholinergic nerve fibers in the feline spinal cord blood vessels were studied by means of amine histofluorescence and acetylcholinesterase (AChE) staining. These patterns were compared with those of the cerebral blood vessels. The anterior spinal artery had a dense network of aminergic and AChE-positive (probably cholinergic) nerve fibers. The posterior spinal vein, in contrast, exhibited only aminergic nerve fibers. Small intraparenchymal blood vessels in the spinal cord also had strongly fluorescent (probably peripheral) aminergic nerve fibers, as well as pial spinal blood vessels. This was a characteristic feature in the spinal cord. The distribution pattern and the density of these two sorts of nerve fibers in the anterior spinal artery varied widely with the individual segment of the spinal cord. The thoracic spinal cord had the lowest number of these nerve fibers of any part of the spinal cord. This fact may explain why the thoracic spinal cord is most susceptible to ischemic change.

The influence of botulinum toxin type A (BTX) on the immunohistochemical characteristics of noradrenergic and cholinergic nerve fibers supplying the porcine urinary bladder wall

2011 ◽  
Vol 14 (2) ◽  
pp. 181-189 ◽  
Author(s):  
E. Lepiarczyk ◽  
A. Bossowska ◽  
J. Kaleczyc ◽  
M. Majewski
Keyword(s):  
Botulinum Toxin ◽  
Urinary Bladder ◽  
Botulinum Toxin Type A ◽  
Bladder Wall ◽  
Botulinum Toxin Type ◽  
Nerve Fibers ◽  
Chemical Coding ◽  
Urinary Bladder Wall ◽  
Muscle Coat ◽  
Cholinergic Nerve Fibers

The influence of botulinum toxin type A (BTX) on the immunohistochemical characteristics of noradrenergic and cholinergic nerve fibers supplying the porcine urinary bladder wall Botulinum toxin (BTX) belongs to a family of neurotoxins which strongly influence the function of autonomic neurons supplying the urinary bladder. Accordingly, BTX has been used as an effective drug in experimental therapies of a range of neurogenic bladder disorders. However, there is no detailed information dealing with the influence of BTX on the morphological and chemical properties of nerve fibres supplying the urinary bladder wall. Therefore, the present study investigated, using double-labeling immunohistochemistry, the distribution, relative frequency and chemical coding of cholinergic and noradrenergic nerve fibers supplying the wall of the urinary bladder in normal female pigs (n=6) and in the pigs (n=6) after intravesical BTX injections. In the pigs injected with BTX, the number of adrenergic (DβH-positive) nerve fibers distributed in the bladder wall (urothelium, submucosa and muscle coat) was distinctly higher while the number of cholinergic (VAChT-positive) nerve terminals was lower than that found in the control animals. Moreover, the injections of BTX resulted in some changes dealing with the chemical coding of the adrenergic nerve fibers. In contrast to the normal pigs, in BTX injected animals the number of DβH/NPY- or DβH/CGRP-positive axons was higher in the muscle coat, and some fibres distributed in the urothelium and submucosa expressed immunoreactivity to CGRP. The results obtained suggest that the therapeutic effects of BTX on the urinary bladder might be dependent on changes in the distribution and chemical coding of nerve fibers supplying this organ.

scholarly journals Lack of mucosal cholinergic innervation is associated with increased risk of enterocolitis in Hirschsprung’s disease

2020 ◽  
Author(s):  
Simone Keck ◽  
Virginie Galati-Fournier ◽  
Urs Kym ◽  
Michèle Moesch ◽  
Jakob Usemann ◽  
...  
Keyword(s):  
Immune Cells ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Nerve Fibers ◽  
Cholinergic Innervation ◽  
Cholinergic Nerve ◽  
Link Type ◽  
Microbial Dysbiosis ◽  
The Impact ◽  
Cholinergic Nerve Fibers

Background & AimsHirschsprung’s disease (HSCR) is a congenital intestinal motility disorder defined by the absence of enteric nervous cells (ganglia). The development of HSCR-associated enterocolitis remains a life-threatening complication. Absence of enteric ganglia implicates extramural innervation of acetylcholine-secreting (cholinergic) nerve fibers. Cholinergic signals have been reported to control excessive inflammation, but the impact on HSCR-associated enterocolitis is unknown.MethodsWe enrolled 44 HSCR patients in a prospective multicenter study and grouped them according to their degree of colonic mucosal cholinergic innervation using immunohistochemistry. The fiber phenotype was correlated with the tissue cytokine profile as well as immune cell frequencies using quantitative reverse-transcribed real-time polymerase chain reaction (qRT-PCR) of whole colonic tissue and fluorescence-activated cell sorting (FACS) analysis of isolated colonic immune cells. Fiber-associated immune cells were identified using confocal immunofluorescence microscopy and characterized by RNA-seq analysis. Microbial dysbiosis was analyzed in colonic patient tissue using 16S rDNA gene sequencing. Finally, the fiber phenotype was correlated with postoperative enterocolitis manifestation.ResultsWe provided evidence that extrinsic mucosal innervation correlated with reduced interleukin (IL)-17 cytokine levels and T-helper-17 (Th17) cell frequencies. Bipolar CD14high macrophages colocalized with neurons and expressed significantly less interleukin-23, a Th17-promoting cytokine. HSCR patients lacking mucosal cholinergic nerve fibers showed microbial dysbiosis and had a higher incidence of postoperative enterocolitis.ConclusionThe mucosal fiber phenotype might serve as a new prognostic marker for enterocolitis development in HSCR patients and may offer an approach to personalized patient care and new future therapeutic options. (www.clinicaltrials.gov accessing number NCT03617640)

scholarly journals Distribution within the choroid of cholinergic nerve fibers from the ciliary ganglion in pigeons

1996 ◽  
Vol 36 (6) ◽  
pp. 775-786 ◽  
Author(s):  
Sherry Cuthbertson ◽  
Jennifer White ◽  
Malinda E.C. Fitzgerald ◽  
Yung-Feng Shih ◽  
Anton Reiner
Keyword(s):  
Nerve Fibers ◽  
Ciliary Ganglion ◽  
Cholinergic Nerve ◽  
Cholinergic Nerve Fibers
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