Update on the Evolving Role of MALDI-TOF MS for Laboratory Diagnosis of Fungal Infections

Abstract Biotyper analysis of Nannizziopsis guarroi, a fatal fungal pathogen in lizards, was described recently. Hypocrealean fungal infections in captive reptiles appear with an increasing frequency during the last decade. Therefore, the aim of this study was to proof Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) as diagnostic tool for the identification of reptile pathogenic hypocrealean fungi. Ten fungal isolates obtained from nine reptiles with fungal glossitis, disseminated visceral mycosis, pneumomycosis, and fungal keratitis were analyzed. Phylogeny consisted of fragments of the large subunit of nuclear encoded ribosomal DNA (D1/D2, LSU) and the internal transcribed spacer region 1 of nuclear encoded ribosomal DNA (ITS1) as well as the protein coding gene translation elongation factor 1 alpha (TEF). Results revealed unanimously two Metarhizium granulomatis genotypes in a total of three isolates, various M. viride genotypes (n = 3), two different Purpureocillium lilacinum isolates as well as one isolate of each P. lavendulum and Beauveria bassiana. Purpureocillium lilacinum and B. bassiana are likewise frequently employed as a mycoinsecticide and mycoacaricide in agriculture on a worldwide scale and have occasionally been reported in man, causing fungal keratitis, sclerokeratitis, nosocomial infections in immunosuppressed patients, as well as cavitary pulmonary disease and cutaneous hyalohyphomycosis in immunocompetent patients. According to the results establishment of Biotyper analysis for faster differentiation of reptile-associated fungal pathogens is entirely justified.

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MALDI-TOF MS: the proteomic approach, the future of fungal identification in India

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20202898 ◽

2020 ◽

Vol 8 (7) ◽

pp. 2575

Author(s):

Mallika Fonseca ◽

Sarala Menon ◽

Praveen Rahi ◽

Prachi Patekar ◽

Abhay S. Chowdhary

Keyword(s):

Yeast Species ◽

Fungal Infections ◽

Tertiary Care ◽

Turnaround Time ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Proteomic Approach ◽

Conventional Methods ◽

Tof Ms

Background: India, being a country where fungal infections are rampant, is urgently in need of effective tools for early and accurate diagnosis of fungal infections. Matrix-assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) is a recent method which has shown potential in identifying clinically important bacterial pathogens as well as clinically important fungi. The main objective of this study was to compare the utility of MALDI-TOF MS for the identification of fungi against that of conventional methods.Methods: The project was carried out in a tertiary care government hospital in India. Fifty clinical isolates comprising mainly various yeast species were subjected to conventional identification (Phenotypic) as well as MALDI-TOF-MS. Their results were further compared.Results: MALDI-TOF MS showed a high concordance with conventional methods while identifying species like C. albicans, C. tropicalis, C. parapsilosis and C. neoformans, although the concordance for species such as Rhodotorula and Trichosporon could only be matched up to genus level.Conclusions: MALDI-TOF MS-based identification is both a rapid and a viable tool for identification of clinically relevant yeast species with good correlation to conventional methods and a quick turnaround time.

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Implementation of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase-Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis

Journal of Clinical Microbiology ◽

10.1128/jcm.00177-15 ◽

2015 ◽

Vol 53 (7) ◽

pp. 2030-2036 ◽

Cited By ~ 35

Author(s):

Xavier Argemi ◽

Philippe Riegel ◽

Thierry Lavigne ◽

Nicolas Lefebvre ◽

Nicolas Grandpré ◽

...

Keyword(s):

Laser Desorption Ionization ◽

Coagulase Negative Staphylococci ◽

Ionization Time ◽

Content Type ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Phenotypic Methods ◽

Tof Ms

The use of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) for staphylococcal identification is now considered routine in laboratories compared with the conventional phenotypical methods previously used. We verified its microbiological relevance for identifying the main species of coagulase-negative staphylococci (CoNS) by randomly selecting 50 isolates. From 1 January 2007 to 31 August 2008, 12,479 staphylococci were isolated with phenotypic methods, of which 4,594 were identified asStaphylococcus aureusand 7,885 were coagulase negative staphylococci. Using MALDI-TOF MS from 1 January 2011 to 31 August 2012, 14,913 staphylococci were identified, with 5,066 asS. aureusand 9,847 as CoNS. MALDI-TOF MS allowed the identification of approximately 85% of the CoNS strains, whereas only 14% of the CoNS strains were identified to the species level with phenotypic methods because they were often considered contaminants. Furthermore, the use of MALDI-TOF MS revealed the occurrence of recently characterizedStaphylococcusspecies, such asS. pettenkoferi,S. condimenti, andS. piscifermentans. Microbiological relevance analysis further revealed that some species displayed a high rate of microbiological significance, i.e., 40% of theS. lugdunensisstrains included in the analysis were associated with infection risk. This retrospective microbiological study confirms the role of MALDI-TOF MS in clinical settings for the identification of staphylococci with clinical consequences. The species distribution reveals the occurrence of the recently identified speciesS. pettenkoferiand putative virulent species, includingS. lugdunensis.

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Identification of Fungi Isolated from Oral Cavity of Patients with HIV Using MALDI–TOF MS

Journal of Clinical Medicine ◽

10.3390/jcm10081570 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1570

Author(s):

Zuzanna Pawlak ◽

Szymon Andrusiów ◽

Magdalena Pajączkowska ◽

Adriana Janczura

Keyword(s):

Fungal Infections ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Common Mistake ◽

Microbiological Methods ◽

Study Results ◽

Identification Of Fungi ◽

Group A ◽

Tof Ms

Background: A growing incidence of invasive fungal infections, especially among immunocompromised patients, has given increased significance to microbiological diagnostics of yeast-like fungi. More accurate and faster fungi identification methods that can compete with classical methods are being searched for. In this paper, classical microbiological methods are compared to MALDI–TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry). Methods: The diagnostic material was collected from buccal mucosa from 98 adults, including 69 with HIV. Only positive cultures were included in the study. Results: Matching results were obtained in 45 samples, and there were nonmatching results in 35 samples, with the majority of these in the study group, constituting 50% of identifications within this group. A particularly common mistake resulting from the use of classical methods is the false identification of C. dubliniensis as C. albicans. Additionally, C. tropicalis proves to be difficult to identify. Conclusions: Our results and literature data suggest that MALDI–TOF MS should be considered an effective alternative to classical methods in terms of fungi identification, especially among HIV-positive patients, due to the different morphology of fungal colonies.

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Influence of storage conditions on MALDI-TOF MS profiling of gingival crevicular fluid: Implications on the role of S100A8 and S100A9 for clinical and proteomic based diagnostic investigations

PROTEOMICS ◽

10.1002/pmic.201500328 ◽

2016 ◽

Vol 16 (6) ◽

pp. 1033-1045 ◽

Cited By ~ 13

Author(s):

Mariaimmacolata Preianò ◽

Giuseppina Maggisano ◽

Nicola Lombardo ◽

Tiziana Montalcini ◽

Sergio Paduano ◽

...

Keyword(s):

Gingival Crevicular Fluid ◽

Storage Conditions ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Crevicular Fluid ◽

Tof Ms ◽

Diagnostic Investigations

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Comparison of MALDI-TOF MS and VITEK 2 system for laboratory diagnosis of Granulicatella and Abiotrophia species causing invasive infections

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2013.07.008 ◽

2013 ◽

Vol 77 (3) ◽

pp. 216-219 ◽

Cited By ~ 16

Author(s):

Paul Ratcliffe ◽

Hong Fang ◽

Ellinor Thidholm ◽

Stina Boräng ◽

Katarina Westling ◽

...

Keyword(s):

Laboratory Diagnosis ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Invasive Infections ◽

Vitek 2 ◽

Tof Ms

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MALDI-TOF/MS biomarkers discovery for pancreatic cancer diagnosis: emerging role of Apolipoprotein A1 and complement C3

Pancreatology ◽

10.1016/j.pan.2012.11.012 ◽

2012 ◽

Vol 12 (6) ◽

pp. 505

Author(s):

A. Padoan ◽

R. Seraglia ◽

P. Fogar ◽

C. Sperti ◽

S. Moz ◽

...

Keyword(s):

Pancreatic Cancer ◽

Cancer Diagnosis ◽

Apolipoprotein A1 ◽

Complement C3 ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

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Inside front cover: Influence of Storage Conditions on MALDI-TOF MS Profiling of Gingival Crevicular Fluid: Implications on the Role of S100A8 and S100A9 for Clinical and Proteomic based Diagnostic Investigations

PROTEOMICS ◽

10.1002/pmic.201670064 ◽

2016 ◽

Vol 16 (6) ◽

pp. NA-NA

Author(s):

Mariaimmacolata Preianò ◽

Giuseppina Maggisano ◽

Nicola Lombardo ◽

Tiziana Montalcini ◽

Sergio Paduano ◽

...

Keyword(s):

Gingival Crevicular Fluid ◽

Storage Conditions ◽

Front Cover ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Crevicular Fluid ◽

Tof Ms ◽

Diagnostic Investigations

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Use of MALDI TOF-MS Method After Lysis Filtration for Rapid Identification of Yeast

Türk Mikrobiyoloji Cemiyeti Dergisi ◽

10.5222/tmcd.2021.04909 ◽

2021 ◽

Author(s):

Sami Eren ◽

Dilek Yeşim Metin ◽

Süheyla Hilmioğlu Polat

Keyword(s):

Fungal Infections ◽

Rapid Identification ◽

Candida Guilliermondii ◽

Candida Spp ◽

Filtration Method ◽

Maldi Tof Ms ◽

Maldi Tof ◽

School Of Medicine ◽

Reliable Methods ◽

Tof Ms

Objective: The early diagnosis of candidosis is very important in fungal infections and to reduce mortality rates, short-term and reliable methods are needed. In this study, the appropriateness of MALDI-TOF MS method after lysis filtration was investigated for the faster identification of Candida spp. Method: 100 Candida isolated from positive blood cultures sent to Ege University, School of Medicine, Medical Microbiology laboratory, were studied with Dalmau plaque, MALDI-TOF MS and after lysis filtration methods. The results were compared with those obtained from the classical MALDI-TOF MS method. Results: According to conventional and MALDI-TOF MS identification methods; 37 Candida albicans, 23 Candida parapsilosis, 17 Candida tropicalis, nine Candida glabrata, six Candida kefyr, three Candida guilliermondii, three Candida dubliniensis and three Candida krusei were identified. After lysis filtration method; 26 C. albicans, nine C. parapsilosis, 13 C. tropicalis, nine C. glabrata, two C. kefyr, three C. dubliniensis, two C. krusei. MALDI-TOF MS method and Dalmau plaque and after lysis filtration method were found to be compatible in 64 strains. Incompatibility was not due to incorrect identification, but spectrum deficiency. Conclusion: The strains, which are not identified as “spectrum deficiency” consist of the species in the database. Sixty-four Candida species were found to be 100% compatible with the standard methods and this method was found to be advantageous for at least 48 hours compared to MALDI-TOF MS method which required subculture and for at least 72 hours compared to Dalmau plaque method.

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