Tetrodotoxin distribution in natural hybrids between the pufferfish species Takifugu rubripes and Takifugu porphyreus

2018 ◽  
Vol 85 (1) ◽  
pp. 237-245
Author(s):  
Ryohei Tatsuno ◽  
Yumi Miyata ◽  
Hiroyuki Yoshikawa ◽  
Yasuko Ino ◽  
Tsubasa Fukuda ◽  
...  
Keyword(s):  
Takifugu Rubripes ◽  
Natural Hybrids

Studies on "Kuchijiro-sho" of cultured tiger puffer Takifugu rubripes. 1. Histopathological findings of cultured tiger puffer Takifugu rubripes naturally infected with "Kuchijiro-sho".

1985 ◽  
Vol 20 (4) ◽  
pp. 495-500 ◽  
Author(s):  
Shinpei WADA ◽  
Yukio FUJIMAKI ◽  
Kishio HATAI ◽  
Saburoh S.KUBOTA ◽  
Masae ISODA
Keyword(s):  
Takifugu Rubripes ◽  
Histopathological Findings ◽  
Tiger Puffer

Voltammetric Detection of Tetrodotoxin Real-Time In Vivo of Mouse Organs using DNA-Immobilized Carbon Nanotube Sensors

2019 ◽  
Vol 15 (5) ◽  
pp. 567-574
Author(s):  
Huck Jun Hong ◽  
Suw Young Ly
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Anticancer Agents ◽  
Cancer Metastasis ◽  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Takifugu Rubripes ◽  
In Vivo Detection ◽  
Voltammetric Detection

Background: Tetrodotoxin (TTX) is a biosynthesized neurotoxin that exhibits powerful anticancer and analgesic abilities by inhibiting voltage-gated sodium channels that are crucial for cancer metastasis and pain delivery. However, for the toxin’s future medical applications to come true, accurate, inexpensive, and real-time in vivo detection of TTX remains as a fundamental step. Methods: In this study, highly purified TTX extracted from organs of Takifugu rubripes was injected and detected in vivo of mouse organs (liver, heart, and intestines) using Cyclic Voltammetry (CV) and Square Wave Anodic Stripping Voltammetry (SWASV) for the first time. In vivo detection of TTX was performed with auxiliary, reference, and working herring sperm DNA-immobilized carbon nanotube sensor systems. Results: DNA-immobilization and optimization of amplitude (V), stripping time (sec), increment (mV), and frequency (Hz) parameters for utilized sensors amplified detected peak currents, while highly sensitive in vivo detection limits, 3.43 µg L-1 for CV and 1.21 µg L-1 for SWASV, were attained. Developed sensors herein were confirmed to be more sensitive and selective than conventional graphite rodelectrodes modified likewise. A linear relationship was observed between injected TTX concentration and anodic spike peak height. Microscopic examination displayed coagulation and abnormalities in mouse organs, confirming the powerful neurotoxicity of extracted TTX. Conclusion: These results established the diagnostic measures for TTX detection regarding in vivo application of neurotoxin-deviated anticancer agents and analgesics, as well as TTX from food poisoning and environmental contamination.

scholarly journals Correction to: Precocious maturation in male tiger pufferfish Takifugu rubripes: genetics and endocrinology

2021 ◽  
Author(s):  
Sota Yoshikawa ◽  
Hisashi Chuda ◽  
Masaomi Hamasaki ◽  
Kazushi Kadomura ◽  
Toshiyuki Yamada ◽  
...  
Keyword(s):  
Takifugu Rubripes ◽  
Tiger Pufferfish

A correction to this paper has been published: https://doi.org/10.1007/s12562-021-01505-w

scholarly journals Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hongwei Yan ◽  
Qi Liu ◽  
Jieming Jiang ◽  
Xufang Shen ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Sex Determination ◽  
High Throughput Sequencing ◽  
Sex Differentiation ◽  
Mature Mirnas ◽  
Takifugu Rubripes ◽  
Sequencing Technology ◽  
Non Coding Rna ◽  
Differentially Expressed Mirnas ◽  
Tiger Pufferfish ◽  
Long Non Coding Rna

AbstractAlthough sex determination and differentiation are key developmental processes in animals, the involvement of non-coding RNA in the regulation of this process is still not clarified. The tiger pufferfish (Takifugu rubripes) is one of the most economically important marine cultured species in Asia, but analyses of miRNA and long non-coding RNA (lncRNA) at early sex differentiation stages have not been conducted yet. In our study, high-throughput sequencing technology was used to sequence transcriptome libraries from undifferentiated gonads of T. rubripes. In total, 231 (107 conserved, and 124 novel) miRNAs were obtained, while 2774 (523 conserved, and 2251 novel) lncRNAs were identified. Of these, several miRNAs and lncRNAs were predicted to be the regulators of the expression of sex-related genes (including fru-miR-15b/foxl2, novel-167, novel-318, and novel-538/dmrt1, novel-548/amh, lnc_000338, lnc_000690, lnc_000370, XLOC_021951, and XR_965485.1/gsdf). Analysis of differentially expressed miRNAs and lncRNAs showed that three mature miRNAs up-regulated and five mature miRNAs were down-regulated in male gonads compared to female gonads, while 79 lncRNAs were up-regulated and 51 were down-regulated. These findings could highlight a group of interesting miRNAs and lncRNAs for future studies and may reveal new insights into the function of miRNAs and lncRNAs in sex determination and differentiation.

scholarly journals Human Homologs of the Putative G Protein-Coupled Membrane Progestin Receptors (mPRα, β, and γ) Localize to the Endoplasmic Reticulum and Are Not Activated by Progesterone

2006 ◽  
Vol 20 (12) ◽  
pp. 3146-3164 ◽  
Author(s):  
Tom Krietsch ◽  
Maria Sofia Fernandes ◽  
Jukka Kero ◽  
Ralf Lösel ◽  
Maria Heyens ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
G Protein ◽  
Cell Lines ◽  
Spotted Seatrout ◽  
Membrane Receptors ◽  
Takifugu Rubripes ◽  
Camp Production ◽  
Intact Cells ◽  
Progestin Receptors ◽  
G Protein Coupled

Abstract The steroid hormone progesterone exerts pleiotrophic functions in many cell types. Although progesterone controls transcriptional activation through binding to its nuclear receptors, it also initiates rapid nongenomic signaling events. Recently, three putative membrane progestin receptors (mPRα, β, and γ) with structural similarity to G protein-coupled receptors have been identified. These mPR isoforms are expressed in a tissue-specific manner and belong to the larger, highly conserved family of progestin and adiponectin receptors found in plants, eubacteria, and eukaryotes. The fish mPRα has been reported to mediate progesterone-dependent MAPK activation and inhibition of cAMP production through coupling to an inhibitory G protein. To functionally characterize the human homologs, we established human embryonic kidney 293 and MDA-MB-231 cell lines that stably express human mPRα, β, or γ. For comparison, we also established cell lines expressing the mPRα cloned from the spotted seatrout (Cynoscion nebulosus) and Japanese pufferfish (Takifugu rubripes). Surprisingly, we found no evidence that human or fish mPRs regulate cAMP production or MAPK (ERK1/2 or p38) activation upon progesterone stimulation. Furthermore, the mPRs did not couple to a highly promiscuous G protein subunit, Gαq5i, in transfection studies or provoke Ca2+ mobilization in response to progesterone. Finally, we demonstrate that transfected mPRs, as well as endogenous human mPRα, localize to the endoplasmic reticulum, and that their expression does not lead to increased progestin binding either in membrane preparations or in intact cells. Our results therefore do not support the concept that mPRs are plasma membrane receptors involved in transducing nongenomic progesterone actions.

Novel mannose-specific lectins found in torafugu, Takifugu rubripes: A review

2006 ◽  
Vol 1 (1) ◽  
pp. 122-127 ◽  
Author(s):  
Shigeyuki Tsutsui ◽  
Masaki Okamoto ◽  
Satoshi Tasumi ◽  
Hiroaki Suetake ◽  
Kiyoshi Kikuchi ◽  
...  
Keyword(s):  
Takifugu Rubripes

scholarly journals Functional characterization of recombinant interleukin (IL)-17A/F1 in the Japanese pufferfish (Takifugu rubripes)

2016 ◽  
Vol 53 ◽  
pp. 79-80 ◽  
Author(s):  
Jun-ichi Hikima ◽  
Koshin Mihara ◽  
Shun Maekawa ◽  
Han-Ching Wang ◽  
Takashi Aoki ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Takifugu Rubripes

INVESTIGATIONS ON RUBBER-BEARING PLANTS: IV. CYTOGENETIC STUDIES IN ASCLEPIAS (TOURN.) L.

1946 ◽  
Vol 24c (3) ◽  
pp. 66-73 ◽  
Author(s):  
Raymond J. Moore
Keyword(s):  
Chromosome Number ◽  
Natural Hybrids ◽  
Rubber Bearing ◽  
Cytogenetic Studies

The chromosome number of eight species and varieties of Asclepias (A. syriaca, A. incarnata and variety pulchra and forma albiflora, A. Sullivantii, A. tuberosa, A. curassavica, A. speciosa) was found to be 2n = 22.Various interspecific pollinations were attempted without success. A cross of A. incarnata with forma albiflora was successful.Possible natural hybrids between A. syriaca and A. speciosa and between A. incarnata and var. pulchra and forma albiflora are described. Two unusual chimaeral plants of A. incarnata that produced several stems of forma albiflora are described.

CYTOLOGY OF POPLAR SPECIES AND NATURAL HYBRIDS

1938 ◽  
Vol 16c (11) ◽  
pp. 445-455 ◽  
Author(s):  
F. H. Peto
Keyword(s):  
Size Distribution ◽  
Genetic Factors ◽  
Chromosome Complement ◽  
Pollen Grains ◽  
Pollen Size ◽  
Cell Nuclei ◽  
Good Pollen ◽  
Natural Hybrids ◽  
Pollen Diameter ◽  
High Degree

Complete analyses of pollen-mother-cell nuclei at first metaphase, percentage good pollen, pollen diameter and pollen-size distribution were determired for the following poplar species and natural hybrids: Populus grandidentata Michx., P. tremuloides Michx., P. eugenei Simon Louis, P. alba L., P. canescens Sm., natural hybrids of P. alba × P. grandidentata and of P. alba × P. tremuloides.Both of the P. alba and two of the four P. canescens trees examined were triploids (2n = 57) while all other trees examined were diploids (2n = 38). Meiotic observations on the natural hybrids indicated a high degree of homology between the chromosomes of P. alba and the native aspens (P. grandidentata and P. tremuloides), since 17 to 19 bivalents were usually found at first metaphase. In collections from one triploid P. canescens and two diploid alba-grandidentata hybrid trees, failure of a high proportion of the chromosomes to pair was attributed to genetic factors limiting pairing, rather than to non-homology.Pollen characters such as percentage good pollen, pollen diameter, and pollen size distribution were, in most cases, not indicative of the chromosome number or pairing relations at first metaphase. Consequently, triploids could not be detected by pollen observations under the conditions of this experiment. In spite of the lack of correlation between first metaphase and pollen observations, abnormally large pollen grains were observed in collections from several of the trees, and these were considered to contain the diploid or unreduced chromosome complement. The tendency for the poplars to produce unreduced pollen grains probably accounts for the number of triploid trees discovered in Canada and Sweden.

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