Development of a polymerase chain reaction (PCR)-based genetic sex identification method in the chub mackerel Scomber japonicus and blue mackerel S. australasicus

2021 ◽  
Author(s):  
Reoto Tani ◽  
Wataru Kawamura ◽  
Tetsuro Morita ◽  
Christophe Klopp ◽  
Marine Milhes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Identification ◽  
Scomber Japonicus ◽  
Chub Mackerel ◽  
Chain Reaction ◽  
Identification Method ◽  
Genetic Sex Identification ◽  
Polymerase Chain

Polymerase Chain Reaction-Based Sex Identification in the Greater Roadrunner

2010 ◽  
Vol 74 (6) ◽  
pp. 1395-1399
Author(s):  
CARLOS A. SANTAMARIA ◽  
SAMUEL KELLEY ◽  
GERRAL G. SCHULZ ◽  
DEAN RANSOM ◽  
LUIS A. HURTADO
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Identification ◽  
Chain Reaction ◽  
Polymerase Chain

Sex identification of pigeons using polymerase chain reaction analysis with simple DNA extraction

2019 ◽  
Vol 12 (2) ◽  
pp. 45-48
Author(s):  
Shao-jie Liang ◽  
Ming-xia Chen ◽  
Chun-qi Gao ◽  
Hui-chao Yan ◽  
Guo-long Zhang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Polymerase Chain Reaction Analysis ◽  
Sex Identification ◽  
Z Chromosome ◽  
W Chromosome ◽  
Chain Reaction ◽  
Assay Time ◽  
Polymerase Chain ◽  
The Cost

Sex identification plays an important role in avian production. Hitherto, it is difficult to distinguish the sexes of monomorphic birds based on their external features. The chromo-helicase-DNA-binding genes contain CHD-W gene and CHD-Z gene, which are located on the W chromosome and Z chromosome, respectively. Since CHD-W gene is unique to females, the polymerase chain reaction can be used for sex identification. However, extracting DNA procedures for verifying the sex is tedious and expensive. To address these disadvantages, the objective of this study was to develop a simple DNA extraction assay to efficiently process blood, liver, and feather samples. The results showed that 2% dimethylsulfoxide was suitable for processing blood, and phosphate-buffered saline was suitable for processing liver and feather samples. The specific primers were designed, and the length of the targets is 474 bp on Z chromosome and 319 bp on W chromosome. The pigeons were identified as females based on the presence of two bands on the gel, and as males based on the presence of one band. Taken together, our results suggested that feather samples were more appropriate than blood or liver for sex identification of pigeons. Compared to the traditional DNA extraction, this method shortened the assay time and reduced the cost.

scholarly journals Sex identification by polymerase chain reaction using a Y-autosome homologous primer set

1993 ◽  
Vol 38 (4) ◽  
pp. 429-431
Author(s):  
Taku Iida ◽  
Yutaka Nakahori ◽  
Karo Tanaka ◽  
Osamu Tsutsumi ◽  
Yuji Taketani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Identification ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Evaluation of the applicability of polymerase chain reaction (PCR) to sex identification in Eurasian blackbirds (Turdus merula)

2009 ◽  
Vol 46 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Andrzej Dybus ◽  
Anna Siemierz ◽  
Dariusz Wysocki ◽  
Iwona Szatkowska ◽  
Magdalena Muszyńska ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Polymorphism ◽  
Bird Species ◽  
Sex Identification ◽  
Chain Reaction ◽  
Turdus Merula ◽  
Polymerase Chain

Evaluation of the applicability of polymerase chain reaction (PCR) to sex identification in Eurasian blackbirds (Turdus merula)Turdus merulais one of most common bird species in Europe. This study verified a method for its sex identification by PCR with P2/P8 primers, based on theCHD1gene polymorphism, proposed in earlier studies as a universal tool for sex identification in most bird species. Although there are some reports that PCR cannot determine sex in Eurasian blackbirds due to a lack of differences in intron lengths ofCHD1-ZandCHD1-Wgenes, our study showed that such a possibility does exist, so it can contribute to an increased understanding of the biology of this species.

Sex identification of forensic specimens by polymerase chain reaction (PCR): Two alternative methods

1991 ◽  
Vol 49 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Atsushi Akane ◽  
Hiroshi Shiono ◽  
Kazuo Matsubara ◽  
Yutaka Nakahori ◽  
Satoko Seki ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Identification ◽  
Alternative Methods ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Sequence of the S10 cDNA from 'McIntosh' Apple and a PCR-digestion Identification Method

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 187-190 ◽  
Author(s):  
Kentaro Kitahara ◽  
Shogo Matsumoto
Keyword(s):  
Polymerase Chain Reaction ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Restriction Enzyme ◽  
Chain Reaction ◽  
Specific Primers ◽  
Amino Acid Sequence Level ◽  
Identification Method ◽  
Polymerase Chain

An S-allele cDNA was cloned from pistils of 'McIntosh' apple (Malus ×domestica Borkh.). The allele, designated Si in Japan and S10 in Europe, is an S-RNase that is very similar (94%) to the S3-RNase at the deduced amino acid sequence level. This allele can be detected by amplification using the polymerase chain reaction (PCR) and specific primers, followed by digestion with restriction enzyme EheI. The S10 allele was discovered in 'Empire', 'Maypole', 'Shinano Red', 'Spencer', and 'Vista Bella'. The S-allele cDNAs sequenced to date are listed with their Japanese and European designations.

Polymerase Chain Reaction–Based Sex Identification in the Greater Roadrunner

2010 ◽  
Vol 74 (6) ◽  
pp. 1395-1399 ◽  
Author(s):  
Carlos A. Santamaria ◽  
Samuel Kelley ◽  
Gerral G. Schulz ◽  
Dean Ransom ◽  
Luis A. Hurtado
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Identification ◽  
Chain Reaction ◽  
Polymerase Chain
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