Laccase Production by a Novel White-Rot Fungus Pseudolagarobasidium acaciicola LA 1 Through Solid-State Fermentation of Parthenium Biomass and Its Application in Dyes Decolorization

2016 ◽  
Vol 7 (6) ◽  
pp. 1427-1435 ◽  
Author(s):  
Anurup Adak ◽  
Rameshwar Tiwari ◽  
Surender Singh ◽  
Satyawati Sharma ◽  
Lata Nain
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Dyes Decolorization

scholarly journals Decolorization of textile industry wastewater in solid state fermentation with Peach-Palm (Bactris gasipaes) residue

2018 ◽  
Vol 78 (4) ◽  
pp. 718-727 ◽  
Author(s):  
J. A. Chicatto ◽  
K. T. Rainert ◽  
M. J. Gonçalves ◽  
C. V. Helm ◽  
D. Altmajer-Vaz ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Industrial Effluent ◽  
Color Removal ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Bactris Gasipaes ◽  
Experimental Conditions ◽  
Peach Palm

Abstract In this work we have assessed the decolorization of textile effluents throughout their treatment in a solid-state fermentation (SSF) system. SSF assays were conducted with peach-palm (Bactris gasipaes) residue using the white rot fungus Ganoderma lucidum EF 31. The influence of the dye concentration and of the amounts of peach-palm residue and liquid phase on both the discoloration efficiency and enzyme production was studied. According to our results, independently of experimental conditions employed, laccase was the main ligninolytic enzyme produced by G. lucidum. The highest laccase activity was obtained at very low effluent concentrations, suggesting the existence of an inhibitory effect of higher concentrations on fungal metabolism. The highest percentage of color removal was reached when 10 grams of peach palm residue was moistened with 60 mL of the final effluent. In control tests carried out with the synthetic dye Remazol Brilliant Blue R (RBBR) decolorization efficiencies about 20% higher than that achieved with the industrial effluent were achieved. The adsorption of RBBR on peach-palm residue was also investigated. Equilibrium tests showed that the adsorption of this dye followed both Langmuir and Freundlich isotherms. Hence, our experimental results indicate that peach-palm residue is suitable substrate for both laccase production and color removal in industrial effluents.

scholarly journals Pengaruh Sumber Karbon pada Produksi Lakase dari Jamur Pelapuk Putih Marasmius sp. dalam Fermentasi Kultur Padat

2018 ◽  
Vol 8 (02) ◽  
pp. 77
Author(s):  
Hendro Risdianto ◽  
Elis Sofianti ◽  
Suraya Suraya ◽  
Sri Harjati Suhardi ◽  
Tjandra Setiadi
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Laccase Activity ◽  
Low Cost ◽  
Carbon Sources ◽  
Ligninolytic Enzymes ◽  
Solid Support ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production

Lakase merupakan salah satu enzim ligninolitik yang memiliki kemampuan mendegradasi lignin. Lakase telah diproduksi menggunakan jamur pelapuk putih Marasmius sp. dalam Fermentasi Kultur Padat (FKP) menggunakan jerami padi sebagai media pertumbuhan. Pengaruh sumber karbon yaitu glukosa, gliserol, dan molase dalam medium produksi lakase digunakan dalam penelitian ini. Konsentrasi 0,5%; 1,0%; dan 2,0% digunakan untuk tiap jenis sumber karbon. Hasil menunjukkan bahwa aktivitas tertinggi lakase diperoleh pada kultivasi hari ke 6-10  dengan masing-masing aktivitas (872,0 U/L (hari ke-6), 1516,67 U/L (hari ke-9) dan 1270,69 U/L (hari ke-10). Aktivitas lakase tertinggi diperoleh pada penggunaan medium gliserol dan molase masing-masing adalah 1422,36 U/L (pada konsentrasi 1%, hari ke-7) dan 113,19 U/L (pada konsentrasi 2%, hari ke-8). Aktivitas tertinggi tersebut sebanding dengan penggunaan medium glukosa. Oleh karena itu, gliserol dan molase dapat digunakan sebagai alternatif sumber karbon untuk produksi lakase dengan fermentasi kultur padat.Kata kunci: glukosa, gliserol, lakase, molase, Marasmius sp., fermentasi kultur padat Influence of Carbon Sources on Laccase Production by White Rot Fungus Marasmius sp. in Solid State FermentationAbstractLaccase is an one of the ligninolytic enzymes that capable to degrade lignin in biomass. Laccase has been produced by white rot fungus Marasmius sp. in Solid State Fermentation (SSF) using rice straw as the solid support media. The influence of carbon sources, i.e. glucose, glycerol and molasses in medium of laccase production were studied in this paper. The concentration of 0.5%, 1.0% and 2.0% were used for each carbon sources. The results showed that the highest lacase activity was obtained within 6-10 days of cultivation. Glucose concentration of 0.5%, 1.0% and 2.0% gave the highest laccase activity were 872.0 U/L (day 6), 1516.67 U/L (day 9) and 1270.69 U/L (day 10) respectively. The highest laccase activity on using glycerol and molasses was 1422.36 U/L (at concentration of 1 % on day 7th) and 1113.19 U/L (at concentration of 2% on day 8th), respectively. This activity was comparable to that of glucose substrate. Therefore, glycerol and molasses gave a potential chance as carbon sources for the strategy on low cost laccase production in solid state fermentation.Keywords: glucose, glycerol, laccase, molasses, Marasmius sp., solid state fermentation. 

Lignocellulolytic activity of Pleurotus ostreatus under solid state fermentation using silage, stover, and cobs of maize

BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 3797-3807
Author(s):  
Magdah Ganash ◽  
Tarek M. Abdel Ghany ◽  
Mohamed A. Al Abboud ◽  
Mohamed M. Alawlaqi ◽  
Husam Qanash ◽  
...  
Keyword(s):  
Solid State ◽  
Incubation Period ◽  
Solid State Fermentation ◽  
Pleurotus Ostreatus ◽  
White Rot Fungi ◽  
Value Added ◽  
Extracellular Protein ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignocellulosic Substrate

Lignocellulolytic white-rot fungi allow the bioconversion of agricultural wastes into value-added products that are used in a myriad of applications. The aim of this work was to use corn residues (Zea mays L.) to produce valuable products under solid-state fermentation (SSF) with Pleurotus ostreatus. White-rot fungus P. ostreatus was isolated from maize silage (MS) and thereafter it was inoculated on MS as substrate and compared with maize stover (MSt) and maize cobs (MC) to determine the best lignocellulosic substrate for the production of lignocellulolytic enzymes and extracellular protein. The MS gave the highest productivity of CMCase (368.2 U/mL), FPase (170.5 U/mL), laccase (11.4 U/mL), and MnPase (6.6 U/mL). This is compared to productivity on MSt of 222 U/mL, 50.2 U/mL, 4.55 U/mL, and 2.57 U/mL, respectively; and productivity on MC at the same incubation period as 150.5 U/mL, 48.2 U/mL, 3.58 U/mL, and 2.5 U/mL, respectively. The levels of enzyme production declined with increasing incubation period after 15 and 20 days using MS and MC, respectively, as substrates. Maximum liberated extracellular protein content (754 to 878 µg/mL) was recorded using MS, while a low amount (343 to 408 µg/mL) was liberated with using MSt and MC.

Solid-state fermentation of lignocellulotic materials for the production of enzymes by the white-rot fungus Trametes hirsuta in a modular bioreactor

2011 ◽  
Vol 11 (4) ◽  
pp. 395-401 ◽  
Author(s):  
Ulrike Böhmer ◽  
Susanne Frömmel ◽  
Thomas Bley ◽  
Matthias Müller ◽  
Katrin Frankenfeld ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
White Rot ◽  
White Rot Fungus ◽  
Trametes Hirsuta

scholarly journals Evaluation of laccase production by two white-rot fungi using solid-state fermentation with different agricultural and forestry residues

BioResources ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 5287-5300
Author(s):  
Qi An ◽  
Cong-Sheng Li ◽  
Jing Yang ◽  
Si-Yu Chen ◽  
Kai-Yue Ma ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Low Cost ◽  
White Rot Fungi ◽  
White Rot ◽  
Laccase Production ◽  
Sophora Japonica ◽  
Significant Difference ◽  
Cottonseed Hull ◽  
Forestry Residues

Pleurotus ostreatus and a newly isolated Ganoderma lingzhi strain were evaluated for their laccase secretion capacity by solid-state fermentation with different agricultural and forestry residues. There was a significant difference among fungi for biosynthetic potential. In principle, the laccase secretion capacity of P. ostreatus CY 568 was stronger than that from G. lingzhi Han 500. Different species of fungi had a preference for agricultural and forestry residues. The presence of cottonseed hull and Populus beijingensis were helpful for accelerating the rate of laccase enzyme production of P. ostreatus CY 568. Cottonseed hull and corncob were useful for improving the production of laccase from G. lingzhi Han 500. Continuous and stable laccase production was found on cottonseed hull by P. ostreatus CY 568 and G. lingzhi Han 500. Maximum laccase activity obtained from P. ostreatus CY 568 on Toona sinensis, Sophora japonica, Salix babylonica, Populus beijingensis, corncob, cottonseed hull, and straw of Oryza sativa was higher than that from G. lingzhi Han 500, and was nearly 1.16-fold, 1.59-fold, 3.32-fold, 1.39-fold, 1.08-fold, 1.08-fold, and 1.36-fold, respectively. These findings will be helpful for developing new productive strains and expanding more species for industrial application to obtain efficient and low-cost laccase.

Sign in / Sign up

Export Citation Format

Share Document