Effectiveness of Mushroom (Pleurotus Pulmonarius) Waste as Natural Coagulant for Kaolin Synthetic Water via Coagulation-Flocculation Process

Coagulation-flocculation process is a widely used method in water treatment. In this study, mushroom waste was selected as natural coagulant to treat kaolin synthetic water via coagulation-flocculation process. Rotten mushroom and mushroom spent were evaluated separately. Functional group of mushroom wastes were analyzed using Fourier Transform Infrared Spectroscopy. Optimum dosage and pH of mushroom wastes and aluminium sulphate in kaolin synthetic water treatment were examined through jar testing. A comparative study on the effectiveness of mushroom wastes and aluminium sulphate in kaolin synthetic water treatment was evaluated in terms of turbidity removal percentage and sludge volume index. Results obtained from jar testing show rotten mushroom and mushroom spent achieved turbidity removal efficiency of 99.73% and 99.25% respectively at optimal pH of 4 and optimal dosage of 5 mg/L whereas 99.91% of turbidity was eliminated by alum under optimum condition of pH 9 and dosage 25 mg/L. Under optimized condition, sludge volume index value obtained by rotten mushroom, mushroom spent and alum were 37.38 mL/g, 40.34 mL/g and 51.58 mL/g respectively. Overall, the finding indicated that mushroom waste could be a potential alternative to chemical coagulant since they are environmentally friendly, effective in turbidity removal, producing less sludge and pH independent.

Cultivation of oyster mushrooms (Pleurotus sp.) using organic waste: an example with Pleurotus pulmonarius (Fr.) Quel.

Pleurotus pulmonarius (Fr.) Quel. is a mushroom species that occurs widely in nature on all continents except Antarctica. It is most common in North America. Its fruiting bodies are characterised by a mild taste and a slight anise aroma. These mushrooms are valued as a source of nutrients and substances with a healing effect. The anticancer, anti-inflammatory and antioxidant properties of P. pulmonarius have been scientifically proven, as well as its strong antihyperglycemic activity. P. pulmonarius is easy to grow because it has a very aggressive mycelium towards cellulosecontaining materials. In Poland, it can be grown on substrates based on cereal straw and various types of organic waste, including agricultural, horticultural, textile and forestry. In intensive crops, the substrates are also enriched with protein and carbohydrates. On an industrial scale, P. pulmonarius is grown primarily in Asia and North America on locally available organic materials.

Deciphering the Amendment-Induced Secretion of Ligninolytic Enzymes by Pleurotus pulmonarius

Pleurotus pulmonarius belonging to the white-rot fungal basidiomycetes group secretes extracellular ligninolytic enzymes for the degradation of agroresidues. The present study was carried out to evaluate the efficacy of different agro-residues for the enhanced production of ligninolytic enzymes and to authenticate their ability by protein analysis. The morphological and molecular sequences of white-rot fungi were characterised. Besides, the efficacy of organic and inorganic amendments in the secretion of ligninolytic enzymes by P. pulmonarius was characterised using SDS-PAGE and native PAGE analysis. The characterised strain of P. pulmonarius secreted enhanced laccase enzyme levels in the liquid medium through supplementation with organic and inorganic amendments. Wheat bran and groundnut cake each @5% enhanced secretions of Laccase, LiP and MnP. Copper sulphate at 150 μM enhanced the laccase enzyme and at 100 μM enhanced the LiP enzyme level by P. pulmonarius. Similarly, supplementation with manganese sulphate at 150 μM enhanced laccase, LiP and MnP enzyme levels compared to control. SDS-PAGE results showed protein banding patterns in the range of 50–85 kDa for the Lac enzyme in samples drawn from wheat bran and groundnut cake-supplemented substrates. Native PAGE results of laccase enzymes also showed that wheat bran (5%) + groundnut cake (5%) + CuSO4 (150 M) + MnSO4 (150 M) induced four laccase isozymes. Supplementing organic and inorganic amendments to the substrates would enhance the secretion of laccase enzyme that would aid in better breakdown of lignin.

Evaluation of the capacity of laccase secretion of four novel isolated white-rot fungal strains in submerged fermentation with lignocellulosic biomass

The capacity of novel isolated white-rot fungi secreting laccase was evaluated for various kinds of lignocellulosic biomass in submerged fermentation. The laccase secreted by Neofomitella fumosipora Han 386 and Pleurotus pulmonarius Han 527 was significantly faster than that by Coriolopsis trogii Han 751 and Coriolopsis sanguinaria An 282. Maximum laccase from N. fumosipora Han 386 on the four kinds of lignocellulosic biomass tested appeared on the first day. This phenomenon indicated that N. fumosipora Han 386 secreted laccase rapidly compared with other tested strains in this study and showed the superiority in the rate of secreting laccase. Based on the maximum laccase activity, the ability of secreting laccase of C. sanguinaria An 282 was superior to other tested novel isolated strains. On the whole, N. fumosipora Han 386 and P. pulmonarius Han 527 preferred Toona sinensis to produce laccase, C. trogii Han 751 preferred to produce laccase on Populus beijingensis, and C. sanguinaria An 282 grown on Sorghum straw was more suitable for secreting laccase. The results will be helpful for developing bioprocesses using various kinds of lignocellulosic biomass for lignocellulolytic enzyme production and enlarging the number of laccase producing strains for industrial application.

A Review on Internet of Things Based Humidifier for Mushroom Cultivation

In this study, grey oyster mushroom (Pleurotus pulmonarius) was cultivated in indoor controlled environment to seeking out the possible Risks of contamination and ways of treatment to avoid the contamination. For this, mushroom was cultivated in providing artificial humidifying and Ventilation system to ensure optimum humidity (80-90%) and fresh air Recirculation in different ways of treatment. The ways of treatment were Included as in position of humidifier, frequency of humidifying, plastic Cork of bags opening part and cleaning of humidifier water container. Maximum percentages of bag contamination (2.5-25.30%), cap Contamination (5.6-30.75%), stalk contamination (4.75-23.25%) and root Contamination (2.6-18.45%) were found in front to front humidifier Position, long humidifying with long interval frequency, without plastic Cork, without cleaning and bi- monthly cleaning of humidifier water Container treatment but no diseases and pest infection was found. Whereas, Very low percentages of contamination (0.1-0.5%) were found in Surrounding humidifying position, short humidifying duration with short Interval frequency, with plastic cork and weekly cleaning of humidifier Water container treatment.