Production, Optimization and Partial Characterization of Bacterial Cellulose from Gluconacetobacter xylinus TJU-D2

2018 ◽  
Vol 11 (5) ◽  
pp. 1681-1690 ◽  
Author(s):  
Renpeng Du ◽  
Yu Wang ◽  
Fangkun Zhao ◽  
Xiaoxiao Qiao ◽  
Qiaozhi Song ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Production Optimization ◽  
Partial Characterization ◽  
Gluconacetobacter Xylinus

Production and characterization of Gluconacetobacter xylinus bacterial cellulose using cashew apple juice and soybean molasses

2020 ◽  
Vol 146 ◽  
pp. 285-289 ◽  
Author(s):  
Erika F. Souza ◽  
Maraysa R. Furtado ◽  
Carlos W.P. Carvalho ◽  
Otniel Freitas-Silva ◽  
Leda M.F. Gottschalk
Keyword(s):  
Bacterial Cellulose ◽  
Apple Juice ◽  
Gluconacetobacter Xylinus ◽  
Cashew Apple Juice ◽  
Cashew Apple

Synthesis, Preparation and Characterization of Amine-Induced Bacterial Cellulose-Poly(Vinyl Alcohol) Semi-Interpenetrating Network Hydrogel

2020 ◽  
Vol 841 ◽  
pp. 238-242
Author(s):  
Joachim Emeka Arikibe ◽  
Roselyn Lata ◽  
David Rohindra
Keyword(s):  
Electron Microscopy ◽  
Bacterial Cellulose ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Gluconacetobacter Xylinus ◽  
Interpenetrating Network ◽  
Equilibrium Water Content ◽  
Network Chain ◽  
Scanning Electron

Bacterial cellulose (BC) was synthesized using Gluconacetobacter xylinus (BCRC 14182). Synthesized BC was powdered and dissolved in Bis(ethylenediamine) copper (II) hydroxide (Cuen) solution to introduce the amine (NH2) group onto the BC network to yield modified BC (mBC) which was then blended with poly (vinyl alcohol) (PVA) and subsequently crosslinked with genipin (Gp). Pristine, modified and crosslinked hydrogels were studied using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and swelling behavior in water. FTIR revealed a distortion on the BC network chain via a reduction in the absorption of OH peak of mBC and the emergence of peaks at 1587 and 1560 cm-1 attributed to N-H stretching of the induced NH2 group. SEM confirmed the 3-D fibril and porous structure of BC which became distorted after modification and crosslinking. The hydrogels showed equilibrium water content of 86.5%, 67.5%, 66.7% and 33.0 % for BC, PVA, mBC-PVA and mBC-PVA-Gp, respectively. The decreased swelling in mBC-PVA-Gp indicated that genipin was able to crosslink the modified BC.

scholarly journals Optimization of production and partial characterization of xylanase from a newly isolated Bacillus amyloliquefaciens

2020 ◽  
Vol 42 ◽  
pp. e9
Author(s):  
Bruno Las-Casas Chaves ◽  
Ana Paula Martinazzo ◽  
Brisabella Coca ◽  
Adriane Nunes De Souza ◽  
Carlos Eduardo Teodoro
Keyword(s):  
Carbon Sources ◽  
Xylanase Activity ◽  
Residual Activity ◽  
Inoculum Size ◽  
Production Optimization ◽  
Partial Characterization ◽  
Birchwood Xylan ◽  
Xylanase Production ◽  
Local Soil

This paper reports the process of production optimization and partial characterization of xylanase from a newly isolated Bacillus amyloliquefacies VR002, isolated from local soil. The microorganism exhibited maximum xylanase production when 1.0% (v/v) of inoculum size was added to culture medium with initial pH 6, 1.0% (w/v) birchwood xylan, at 35 °C after 48h of incubation. Xylanase production in different carbon sources apart from birchwood xylan and xylose did not show high production levels. Optimum pH for xylanase activity was 6.0. The enzyme was alkali-stable and retained 100% of residual activity over the pH range from 6.0 to 10.0 for 24 h at 25°C. Optimum temperature for enzyme activity was 55°C. Xylanase was 100% stable at 4°C and 25°C even after 24h of incubation, a desirable characteristic for enzyme storage. Moreover, best crude extract volume and time reaction were found to be 10 µL and 5 min, respectively. After optimization of production and activity parameters, an increase of nearly 60-fold in xylanase activity (44.12 ± 4.36 U/mL) was achieved. Characteristics of B. amyloliquefaciens VR002 xylanase are particularly desirable for biotechnological applications

scholarly journals Production, Optimization and Partial Characterization of Thermostable and Alkaline Amylase from Bacillus licheniformis KSU-6

2016 ◽  
Vol 18 (06) ◽  
pp. 1188-1194 ◽  
Author(s):  
Krishnasamy Nithya ◽  
Chinnasamy Muthukumar ◽  
Dharumadurai Dhanasekaran ◽  
Shine Kadaikunnan ◽  
Naiyf S. Alharbi ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
Production Optimization ◽  
Partial Characterization ◽  
Alkaline Amylase
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