Characterization of water-soluble exopolysaccharides from Gluconacetobacter xylinus and their impacts on bacterial cellulose crystallization and ribbon assembly

Cellulose ◽  
2014 ◽  
Vol 21 (6) ◽  
pp. 3965-3978 ◽  
Author(s):  
Lin Fang ◽  
Jeffrey M. Catchmark
2020 ◽  
Vol 146 ◽  
pp. 285-289 ◽  
Author(s):  
Erika F. Souza ◽  
Maraysa R. Furtado ◽  
Carlos W.P. Carvalho ◽  
Otniel Freitas-Silva ◽  
Leda M.F. Gottschalk

2018 ◽  
Vol 11 (5) ◽  
pp. 1681-1690 ◽  
Author(s):  
Renpeng Du ◽  
Yu Wang ◽  
Fangkun Zhao ◽  
Xiaoxiao Qiao ◽  
Qiaozhi Song ◽  
...  

2020 ◽  
Vol 841 ◽  
pp. 238-242
Author(s):  
Joachim Emeka Arikibe ◽  
Roselyn Lata ◽  
David Rohindra

Bacterial cellulose (BC) was synthesized using Gluconacetobacter xylinus (BCRC 14182). Synthesized BC was powdered and dissolved in Bis(ethylenediamine) copper (II) hydroxide (Cuen) solution to introduce the amine (NH2) group onto the BC network to yield modified BC (mBC) which was then blended with poly (vinyl alcohol) (PVA) and subsequently crosslinked with genipin (Gp). Pristine, modified and crosslinked hydrogels were studied using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and swelling behavior in water. FTIR revealed a distortion on the BC network chain via a reduction in the absorption of OH peak of mBC and the emergence of peaks at 1587 and 1560 cm-1 attributed to N-H stretching of the induced NH2 group. SEM confirmed the 3-D fibril and porous structure of BC which became distorted after modification and crosslinking. The hydrogels showed equilibrium water content of 86.5%, 67.5%, 66.7% and 33.0 % for BC, PVA, mBC-PVA and mBC-PVA-Gp, respectively. The decreased swelling in mBC-PVA-Gp indicated that genipin was able to crosslink the modified BC.


Food Research ◽  
2021 ◽  
Vol 5 (6) ◽  
pp. 204-210
Author(s):  
S. Agustin ◽  
E.T. Wahyuni ◽  
Suparmo ◽  
Supriyadi ◽  
M.N. Cahyanto

Biocomposite of bacterial cellulose-alginate has been developed for use as food packaging material. This study aims to understand the physical and mechanical properties of the biocomposite produced from static fermentation of Gluconacetobacter xylinus InaCC B404 in media supplemented with alginate. The strain was grown in a medium containing alginate at a concentration of 0.4, 0.8, and 1.2% w/v at 30oC for 7 days. The SEM images showed that bacterial cellulose produced in a medium supplemented with alginate had a denser structure of fibril network and a smaller pore size than the control one. The structure change was due to interactions through hydrogen bonds between bacterial cellulose and alginate proven by FTIR spectra, resulting in a decrease in crystallinity and crystallite size of bacterial cellulose. It led to the decrease in tensile and tear strength of the resulting biocomposite. Alginate also causes biocomposite to have higher water vapour permeability values.


Author(s):  
B. J. Grenon ◽  
A. J. Tousimis

Ever since the introduction of glutaraldehyde as a fixative in electron microscopy of biological specimens, the identification of impurities and consequently their effects on biologic ultrastructure have been under investigation. Several reports postulate that the impurities of glutaraldehyde, used as a fixative, are glutaric acid, glutaraldehyde polymer, acrolein and glutaraldoxime.Analysis of commercially available biological or technical grade glutaraldehyde revealed two major impurity components, none of which has been reported. The first compound is a colorless, water-soluble liquid with a boiling point of 42°C at 16 mm. Utilizing Nuclear Magnetic Resonance (NMR) spectroscopic analysis, this compound has been identified to be — dihydro-2-ethoxy 2H-pyran. This impurity component of the glutaraldehyde biological or technical grades has an UV absorption peak at 235nm. The second compound is a white amorphous solid which is insoluble in water and has a melting point of 80-82°C. Initial chemical analysis indicates that this compound is an aldol condensation product(s) of glutaraldehyde.


Author(s):  
Aline Krindges ◽  
Vanusca Dalosto Jahno ◽  
Fernando Morisso

Incorporation studies of particles in different substrates with herbal assets growing. The objective of this work was the preparation and characterization of micro/nanoparticles containing cymbopogon nardus essential oil; and the incorporation of them on bacterial cellulose. For the development of the membranes was used the static culture medium and for the preparation of micro/nanoparticles was used the nanoprecipitation methodology. The incorporation of micro/nanoparticles was performed on samples of bacterial cellulose in wet and dry form. For the characterization of micro/nanoparticles were carried out analysis of SEM, zeta potential and particle size. For the verification of the incorporation of particulate matter in cellulose, analyses were conducted of SEM and FTIR. The results showed that it is possible the production and incorporation of micro/nanoparticles containing essential oil in bacterial cellulose membranes in wet form with ethanol.


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