scholarly journals Optimization of production and partial characterization of xylanase from a newly isolated Bacillus amyloliquefaciens

2020 ◽  
Vol 42 ◽  
pp. e9
Author(s):  
Bruno Las-Casas Chaves ◽  
Ana Paula Martinazzo ◽  
Brisabella Coca ◽  
Adriane Nunes De Souza ◽  
Carlos Eduardo Teodoro
Keyword(s):  
Carbon Sources ◽  
Xylanase Activity ◽  
Residual Activity ◽  
Inoculum Size ◽  
Production Optimization ◽  
Partial Characterization ◽  
Birchwood Xylan ◽  
Xylanase Production ◽  
Local Soil

This paper reports the process of production optimization and partial characterization of xylanase from a newly isolated Bacillus amyloliquefacies VR002, isolated from local soil. The microorganism exhibited maximum xylanase production when 1.0% (v/v) of inoculum size was added to culture medium with initial pH 6, 1.0% (w/v) birchwood xylan, at 35 °C after 48h of incubation. Xylanase production in different carbon sources apart from birchwood xylan and xylose did not show high production levels. Optimum pH for xylanase activity was 6.0. The enzyme was alkali-stable and retained 100% of residual activity over the pH range from 6.0 to 10.0 for 24 h at 25°C. Optimum temperature for enzyme activity was 55°C. Xylanase was 100% stable at 4°C and 25°C even after 24h of incubation, a desirable characteristic for enzyme storage. Moreover, best crude extract volume and time reaction were found to be 10 µL and 5 min, respectively. After optimization of production and activity parameters, an increase of nearly 60-fold in xylanase activity (44.12 ± 4.36 U/mL) was achieved. Characteristics of B. amyloliquefaciens VR002 xylanase are particularly desirable for biotechnological applications

Characterization of cellulase-free xylanases from the newly isolated Bacillus sp. strain BP-23

1993 ◽  
Vol 39 (12) ◽  
pp. 1162-1166 ◽  
Author(s):  
A. Blanco ◽  
F. I. J. Pastor
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Xylanase Activity ◽  
Hydrolytic Activity ◽  
Bacillus Sp ◽  
Birchwood Xylan ◽  
Xylanase Production ◽  
Ph Values ◽  
Wide Range ◽  
Molecular Masses

A Bacillus strain with xylanase activity has been isolated. Maximum xylanase production was obtained when the strain was cultured in media supplemented with birchwood xylan or rice straw; production was repressed by glucose and xylose. The optimal temperature and pH for xylanase activity were 45–50 °C and 5.5–7.5, respectively. Crude xylanase was highly stable at a wide range of pH values, retaining 100% of the activity after 24 h of incubation at 37 °C in buffer at pH 10.0. Analysis by polyacrylamide gel electrophoresis and zymogram techniques showed four xylanase activity bands with apparent molecular masses of 32, 48, 61, and 66 kDa. The most active of them (molecular mass 32 kDa) apparently corresponded to a xylanase with an isoelectric point (pI) of 9.3 in isoelectrofocusing gels developed as zymograms. Four other bands with xylanase activity were detected at pIs of 7.7, 5.6, 5.0, and 4.5. Analysis for carboxymethylcellulase activity revealed that only the band of 48 kDa and the band with a pI of 7.7 showed hydrolytic activity against the cellulosic substrate.Key words: Bacillus sp., xylanase, isolation.

scholarly journals Production and Partial Characterization of an Alkaline Xylanase from a Novel FungusCladosporium oxysporum

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Guo-Qiang Guan ◽  
Peng-Xiang Zhao ◽  
Jin Zhao ◽  
Mei-Juan Wang ◽  
Shu-Hao Huo ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Wheat Bran ◽  
Internal Transcribed Spacer ◽  
Gene Sequence ◽  
Agricultural Waste ◽  
Xylanase Activity ◽  
Maximum Activity ◽  
Alkaline Proteases ◽  
Xylanase Production

A new fungusCladosporium oxysporumGQ-3 producing extracellular xylanase was isolated from decaying agricultural waste and identified based on the morphology and comparison of internal transcribed spacer (ITS) rDNA gene sequence.C. oxysporumproduced maximum xylanase activity of 55.92 U/mL with wheat bran as a substrate and NH4Cl as a nitrogen source. Mg2+improvedC. oxysporumxylanase production.Partially purified xylanase exhibited maximum activity at 50°C and pH 8.0, respectively, and showed the stable activity after 2-h treatment in pH 7.0–8.5 or below 55°C. Mg2+enhanced the xylanase activity by 2% while Cu2+had the highest inhibition ratio of 57.9%. Furthermore,C. oxysporumxylanase was resistant to most of tested neutral and alkaline proteases. Our findings indicated thatCladosporium oxysporumGQ-3 was a novel xylanase producer, which could be used in the textile processes or paper/feed industries.

Purification and Partial Characterization of Cellulase Produced by Aspergillus niger Cultured on Vitellaria paradoxa shells

2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Abdulhakeem Olarewaju Sulyman ◽  
Yusuf A Iyanda ◽  
Afolabi Olaniyi Opasola ◽  
OtunOla Adedayo ◽  
Raliat Abimbola Aladodo
Keyword(s):  
Aspergillus Niger ◽  
Specific Activity ◽  
Gel Filtration ◽  
Inoculum Size ◽  
Partial Characterization ◽  
Effect Of Temperature ◽  
Vitellaria Paradoxa ◽  
Endoglucanase Activity ◽  
Ammonium Sulphate Precipitation

This research investigated the purification and partial characterization of cellulase produced by Aspergillus niger cultured on Vitellaria paradoxa shells. Cellulase (endoglucanase) from A. niger was produced under optimum fermentation conditions at 35 °C, pH 4.7, V. paradoxa, 4 g/L, inoculum size of 10 mm and the fermentation media incubated for 120 hours. The crude endoglucanase obtained were partially purified by subjecting to ammonium sulphate precipitation, dialysis and gel filtration chromatography for further purification. The effect of temperature and pH on the activity of purified endoglucanase was determined. Cellulase was purified to 734.33 folds by Sephadex G-100 column chromatography with a specific activity and yield of 4.406 U/mg and 63.03% respectively. Fractions 4 and 7 contained the highest endoglucanase activity out of 18 fractions collected and the two fractions were pooled for further analysis. The activity of purified endoglucanase was optimum at a temperature of 40 °C and pH 5. Therefore, the purified endoglucanase produced may be explored in detergent industry.

Production, Optimization and Partial Characterization of Bacterial Cellulose from Gluconacetobacter xylinus TJU-D2

2018 ◽  
Vol 11 (5) ◽  
pp. 1681-1690 ◽  
Author(s):  
Renpeng Du ◽  
Yu Wang ◽  
Fangkun Zhao ◽  
Xiaoxiao Qiao ◽  
Qiaozhi Song ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Production Optimization ◽  
Partial Characterization ◽  
Gluconacetobacter Xylinus

scholarly journals Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Abbas Akhavan Sepahy ◽  
Shokoofeh Ghazi ◽  
Maryam Akhavan Sepahy
Keyword(s):  
Agricultural Waste ◽  
Xylanase Activity ◽  
Nitrogen Sources ◽  
Cost Effective ◽  
Inoculum Size ◽  
Alkaline Condition ◽  
Xylanase Production ◽  
Bacillus Mojavensis ◽  
Oat Bran ◽  
Higher Temperature

A xylanase producer Bacillus mojavensis strain, called AG137, isolated from cotton farm (Kashan-Iran). The optimal xylanase activity reached at 55∘C & pH 9.0. Enzyme yield was studied using a medium with different agricultural wastes as inducers. Xylanase production of about 249.308 IU/mL was achieved at pH 8 and 37∘C, within 48 h submerged fermentation in enzyme production medium supplemented with 2% (w/v) oat bran as an optimum carbon source. A mixture of 1% (w/v) yeast extract and 1% (w/v) tryptone as optimum nitrogen sources, agitation speed 200 rpm, and inoculum size 2% (v/v) were the optimums for maximum production. Accordingly, xylanase yield from 194.68 IU/mL under non-optimized fermentation condition enhanced to 302.466 IU/mL in optimized condition. Screened xylanase is thermostable, presenting 70% stability at 60∘C during 30 min. Further enzyme incubation in higher temperature caused a decrease in the residual enzyme activity, yet it retained 68%–50% of its activity after 1 hour from 45∘C to 55∘C. Besides, it is stable in pH 9 and 10, maintaining over 70% of its activity for 2 h. The enzyme also could preserve 71% and 63% of its initial activity after 3 hours of pre-incubation in the same alkaline condition. Produced xylanase therefore was introduced as an alkaline-active and stable one, displaying suitable thermostability feature, confirmed by HPLC analysis. Hence, all xylanase properties highlight its promising uses in industrial scale.

Screening for xylanase and β-xylosidase production from wood-inhabiting Penicillium strains for potential use in biotechnological applications

Holzforschung ◽  
2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Jaejung Lee ◽  
Yeongseon Jang ◽  
Hanbyul Lee ◽  
Sangjoon Lee ◽  
Gyu-Hyeok Kim ◽  
...  
Keyword(s):  
Paper Industry ◽  
Carbon Sources ◽  
Xylanase Activity ◽  
Cellulase Activity ◽  
Pulp And Paper Industry ◽  
Biotechnological Applications ◽  
Birchwood Xylan ◽  
Beechwood Xylan ◽  
Filter Paper Assay ◽  
Potential Use

Abstract Experiments were performed to find potential sources for enzyme production for the pulp and paper industry and for biological ethanol production by screening the cellulase, xylanase and β-xylosidase activities of 36 species of Penicillium isolated from various wood materials in Korea. Rice straw powder (RiceP), birchwood xylan (BirchX), and beechwood xylan (BeechX) were supplied as individual carbon sources for the Penicillium species. All Penicillium species tested in this study showed little cellulase activity, but some species exhibited remarkably high xylanase and β-xylosidase activities, as determined by a filter paper assay. P. oxalicum showed the greatest xylanase activity on RiceP (158.70 U ml-1). On the other hand, P. brevicompactum produced the highest active β-xylosidase on BirchX (6.25 U ml-1).

scholarly journals Influence of some sugars on xylanase production by Aspergillus awamori in solid state fermentation

2002 ◽  
Vol 45 (4) ◽  
pp. 431-437 ◽  
Author(s):  
Judith Liliana Solórzano Lemos ◽  
Nei Pereira Junior
Keyword(s):  
Solid State ◽  
Carbon Source ◽  
Sugar Cane ◽  
Sugar Cane Bagasse ◽  
Aspergillus Awamori ◽  
Birchwood Xylan ◽  
Xylanase Production ◽  
Low Levels ◽  
Xylosidase Activity

Aspergillus awamori showed high extracellular endoxylanase (100 U/ml) and beta-xylosidase activities (3.5 U/ml) when grown on milled sugar cane bagasse as the principal carbon source without treatment. Partial characterization of xylanases showed that the apparent values of Km were 3.12 ± 0.05 mg/ml for endoxylanase (in birchwood xylan) and 0.45 ± 0.05 mM for beta-xylosidase (in p -nitrophenyl beta-D-xylanopiranoside). Corresponding values of Vmax were 6.63 ± 0.02 and 0.078 ± 0.02 mumol/min. Gradual repression of endoxylanase activity was observed when increasing concentrations of glucose and xylose (1, 2, 4, 6 and 8 g of carbohydrate / 4 g of sugar cane bagasse) were added to production media. In contrast, beta-xylosidase activity was stimulated using low levels of carbohydrates (1 g xylose or glucose/ 4 g of sugar cane bagasse).

Effect of Carbon and Nitrogen Ratio, Mineral Solution & Inoculum Size in the Production of Xylanase Using Oil Palm Leaf

2015 ◽  
Vol 1113 ◽  
pp. 273-278
Author(s):  
I. Norazlina ◽  
K.H. Ku Halim ◽  
Shareena Fairuz Abd Manaf ◽  
Muhammad Afiquddin Abu Bakar
Keyword(s):  
Oil Palm ◽  
Xylanase Activity ◽  
Inoculum Size ◽  
Optimum Size ◽  
Xylanase Production ◽  
Carbon And Nitrogen ◽  
Mineral Solution ◽  
Nitrogen Ratio ◽  
Fermentation Parameters ◽  
Palm Leaf

The production of xylanase by Aspergillusniger ATCC 16404 via solid state fermentation (SSF) system using oil palm leaves (OPL) as substrate was investigated. Fermentation parameters studied using one factor at a time (OFAT) technique, were carbon-nitrogen (C/N) ratio, mineral solution size and inoculums size. It was found that the optimum C/N ratio was at 0.4 with xylanase activity at 16.046 U/min. Meanwhile, the optimum size for both mineral solution size and inoculum size were at 1 ml with the xylanase activity recorded at 14.500 U/min and 19.057 U/min respectively. This shows that that the utilization of OPL as substrates in xylanase production using Aspergillusniger ATCC 16404 was a successful.

scholarly journals Production, Purification, and Characterization of a MajorPenicillium glabrumXylanase Using Brewer's Spent Grain as Substrate

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Adriana Knob ◽  
Susan Michelz Beitel ◽  
Diana Fortkamp ◽  
César Rafael Fanchini Terrasan ◽  
Alex Fernando de Almeida
Keyword(s):  
Xylanase Activity ◽  
Stationary Condition ◽  
Purification And Characterization ◽  
Xylanase Production ◽  
Brewer’S Spent Grain ◽  
Agroindustrial Waste ◽  
Sds Page ◽  
Exclusion Chromatography ◽  
Spent Grain

In recent decades, xylanases have been used in many processing industries. This study describes the xylanase production byPenicillium glabrumusing brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained whenP. glabrumwas grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase fromP. glabrumwas purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn2+and the reducing agentsβ-mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg2+, Zn2+, and Cu2+as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost.

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