Development of quantitative PCR assays for the detection and quantification of lake sturgeon (Acipenser fulvescens) environmental DNA

2018 ◽  
Vol 12 (1) ◽  
pp. 17-19 ◽  
Author(s):  
Michael E. Yusishen ◽  
Frances-Claire Eichorn ◽  
W. Gary Anderson ◽  
Margaret F. Docker
Keyword(s):  
Quantitative Pcr ◽  
Environmental Dna ◽  
Lake Sturgeon ◽  
Acipenser Fulvescens ◽  
Pcr Assays ◽  
Detection And Quantification

scholarly journals Quantitative PCR assays for detection of five Arctic fish species: Lota lota, Cottus cognatus, Salvelinus alpinus, Salvelinus malma, and Thymallus arcticus from environmental DNA

2017 ◽  
Author(s):  
Torrey W. Rodgers ◽  
John. R. Olson ◽  
Stephen L. Klobucar ◽  
Karen. E. Mock
Keyword(s):  
Quantitative Pcr ◽  
Salvelinus Alpinus ◽  
Environmental Dna ◽  
North Slope ◽  
Cottus Cognatus ◽  
The North ◽  
North Slope Of Alaska ◽  
Arctic Fish ◽  
Thymallus Arcticus ◽  
Pcr Assays

AbstractThe North Slope of Alaska contains arctic fish populations that are important for subsistence of local human populations, and are under threat from natural resource extraction and climate change. We designed and evaluated four quantitative PCR assays for the detection of environmental DNA from five Alaskan fish species present on the North Slope of Alaska: burbot (Lota lota), arctic char (Salvelinus alpinus), Dolly Varden (Salvelinus malma), arctic grayling (Thymallus arcticus), and slimy sculpin (Cottus cognatus). All assays were designed and tested for species specificity and sensitivity, and all assays detected target species from filtered water samples collected from the field. These assays will enable efficient and economical detection and monitoring of these species in lakes and rivers. This in turn will provide managers with improved knowledge of current distributions and future range shifts associated with climate and development threats, enabling more timely management.

scholarly journals Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

10.3791/61825 ◽  
2020 ◽  
Author(s):  
Katy E. Klymus ◽  
Dannise V. Ruiz Ramos ◽  
Nathan L. Thompson ◽  
Catherine A. Richter
Keyword(s):  
Quantitative Pcr ◽  
Environmental Dna ◽  
Pcr Assays ◽  
Species Specific

Developing quantitative PCR assays to detect threatened and invasive freshwater turtles in Hong Kong using environmental DNA

2019 ◽  
Vol 12 (2) ◽  
pp. 293-300
Author(s):  
Ivan P. Y. Lam ◽  
Yik-Hei Sung ◽  
Liu Lin ◽  
Jonathan J. Fong
Keyword(s):  
Hong Kong ◽  
Quantitative Pcr ◽  
Environmental Dna ◽  
Freshwater Turtles ◽  
Pcr Assays

scholarly journals Application of Quantitative-PCR to Monitor Netpen Sites in British Columbia (Canada) for Tenacibaculum Species

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 414
Author(s):  
Joseph P. Nowlan ◽  
Scott R. Britney ◽  
John S. Lumsden ◽  
Spencer Russell
Keyword(s):  
Atlantic Salmon ◽  
Quantitative Pcr ◽  
Salmo Salar ◽  
Bacterial Load ◽  
Temporal Distribution ◽  
Quality Parameters ◽  
Antimicrobial Use ◽  
Live Fish ◽  
Dead Fish ◽  
Pcr Assays

Tenacibaculum are frequently detected from fish with tenacibaculosis at aquaculture sites; however, information on the ecology of these bacteria is sparse. Quantitative-PCR assays were used to detect T. maritimum and T. dicentrarchi at commercial Atlantic salmon (Salmo salar) netpen sites throughout several tenacibaculosis outbreaks. T. dicentrarchi and T. maritimum were identified in live fish, dead fish, other organisms associated with netpens, water samples and on inanimate substrates, which indicates a ubiquitous distribution around stocked netpen sites. Before an outbreak, T. dicentrarchi was found throughout the environment and from fish, and T. maritimum was infrequently identified. During an outbreak, increases in the bacterial load in were recorded and no differences were recorded after an outbreak supporting the observed recrudescence of mouthrot. More bacteria were recorded in the summer months, with more mortality events and antibiotic treatments, indicating that seasonality may influence tenacibaculosis; however, outbreaks occurred in both seasons. Relationships were identified between fish mortalities and antimicrobial use to water quality parameters (temperature, salinity, dissolved oxygen) (p < 0.05), but with low R2 values (<0.25), other variables are also involved. Furthermore, Tenacibaculum species appear to have a ubiquitous spatial and temporal distribution around stocked netpen sites, and with the potential to induce disease in Atlantic salmon, continued research is needed.

scholarly journals Response in Growth, Scute Development, and Whole-Body Ion Composition of Acipenser fulvescens Reared in Water of Differing Chemistries

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1419
Author(s):  
Janet Genz ◽  
Rachael Hicks
Keyword(s):  
River Water ◽  
Growth And Development ◽  
Lake Sturgeon ◽  
Whole Body ◽  
Acipenser Fulvescens ◽  
Water Type ◽  
Ion Composition ◽  
Ion Concentrations ◽  
Before And After ◽  
Yolk Absorption

In fishes, environmental ion availability can have substantial effects on growth and development. This study examined the development of Lake Sturgeon in response to the varying environmental ion availability that they experience as part of a conservation stocking program. We reared sturgeon in natural water from the Coosa River, which had higher concentrations of Mg2+, Na+, and Zn2+ than standard hatchery conditions, while [Ca2+] at the Warm Springs National Fish Hatchery was 2× higher than in the Coosa River. Eggs were hatched in each water type and the larvae were sampled at time points before and after yolk absorption during the first 8 weeks of development. Total length and weight in WSNFH larvae were significantly higher than larvae in Coosa River water starting at 8 dph, indicating that growth was dependent on the different environmental ion levels. Concentrations of the ions of interest were also determined for whole-body acid digests of the exposed Lake Sturgeon. We found that Lake Sturgeon reared in Coosa River water had significantly higher magnesium and zinc than Lake Sturgeon reared in WSNFH water (p < 0.05), while calcium was significantly higher in WSNFH than Coosa River water. This difference shows that different environmental ion concentrations also impact the overall development of larval Lake Sturgeon.

Sign in / Sign up

Export Citation Format

Share Document