Using environmental DNA to detect Hypophthalmichthys molitrix during the spawning period in the Yangtze River

2018 ◽  
Vol 12 (1) ◽  
pp. 37-39
Author(s):  
Rui Ruan ◽  
Dengqiang Wang ◽  
Huamei Yue ◽  
Chuangju Li ◽  
Daqing Chen ◽  
...  
Fishes ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Hui Jia ◽  
Hui Zhang ◽  
Weiwei Xian

Surveys and assessments based on environmental DNA are not only efficient and time-saving, but also cause less harm to monitoring targets. Environmental DNA has become a common tool for the assessment and monitoring of aquatic organisms. In this study, we investigated fish resources in the Yangtze River mainstream using environmental DNA, and the variations in fish during two seasons (spring and autumn) were compared. The results showed that 13 species were identified in spring, and nine species of fish were identified in autumn. The fish with higher eDNA detection were Sinibotia superciliaris, Tachysurus fulvidraco, Cyprinus carpio, Ctenopharyngodon Idella, Monopterus albus, Acanthogobius hasta, Saurogobio dabryi, Oncorhynchus mykiss, Mugil cephalus, Odontamblyopus rubicundus. Seasonal variation between spring and autumn was not significant, and the environmental factors had different effects on fish assemblages during the two seasons. Our study used the eDNA technique to monitor the composition of fish in the spring and autumn in the Yangtze River mainstream, providing a new technology for the long-term management and protection of fishery resources in the region. Of course, problems such as pollution and insufficient databases are the current shortcomings of environmental DNA, which will be the focus of our future research and study.


PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0221120
Author(s):  
Yongkai Tang ◽  
Yunsheng Wu ◽  
Kai Liu ◽  
Jianlin Li ◽  
Hongxia Li ◽  
...  

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dan Yu ◽  
Zhongyuan Shen ◽  
Tao Chang ◽  
Sha Li ◽  
Huanzhang Liu

Abstract Background To determine the presence and abundance of an aquatic species in large waterbodies, especially when populations are at low densities, is highly challenging for conservation biologists. Environmental DNA (eDNA) has the potential to offer a noninvasive and cost-effective method to complement traditional population monitoring, however, eDNA has not been extensively applied to study large migratory species. Chinese sturgeon (Acipenser sinensis), is the largest anadromous migratory fish in the Yangtze River, China, and in recent years its population has dramatically declined and spawning has failed, bringing this species to the brink of extinction. In this study, we aim to test the detectability of eDNA methods to determine the presence and relative abundance of reproductive stock of the species and whether eDNA can be used as a tool to reflect behavioral patterns. Chinese sturgeon eDNA was collected from four sites along the spawning ground across an eight month period, to investigate the temporal and spatial distribution using droplet digital PCR (ddPCR). Results We designed a pair of specific primers for Chinese sturgeon and demonstrated the high sensitivity of ddPCR to detect and quantify the Chinese sturgeon eDNA concentration with the limit of detection 0.17 copies/μl, with Chinese sturgeon eDNA been intermittently detected at all sampling sites. There was a consistent temporal pattern among four of the sampling sites that could reflect the movement characteristics of the Chinese sturgeon in the spawning ground, but without a spatial pattern. The eDNA concentration declined by approximately 2–3 × between December 2018 and December 2019. Conclusions The results prove the efficacy of eDNA for monitoring reproductive stock of the Chinese sturgeon and the e decreased eDNA concentration reflect that Chinese sturgeon may survive with an extremely small number of reproductive stock in the Yangtze River. Accordingly, we suggest future conservation measures should focus on both habitat restoration and matured fish restocking to ensure successful spawning. Overall, this study provides encouraging support for the application of eDNA methods to monitor endangered aquatic species.


2004 ◽  
Vol 88 (8) ◽  
pp. 59-64
Author(s):  
Changyu Shao ◽  
Qinger Deng

2014 ◽  
Vol 21 (6) ◽  
pp. 688-698
Author(s):  
Sun Shasha ◽  
Tang Wenqiao ◽  
Guo Hongyi ◽  
Li Huihua ◽  
Liu Dong ◽  
...  

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